Browse by DISSEMINATION: Published

SASDV64 – Serine hydrolase FphH with short chain esterase activity (Apo FphH)

Alpha/beta fold hydrolase experimental SAS data
DAMFILT model
Sample: Alpha/beta fold hydrolase monomer, 28 kDa Staphylococcus aureus protein
Buffer: 100 mM NaCl, 10 mM HEPES, pH: 7.6
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2024 Apr 11
Similar but Distinct—Biochemical Characterization of the Staphylococcus aureus Serine Hydrolases FphH  and FphI Proteins: Structure, Function, and Bioinformatics (2024)
Fellner M, Randall G, Bitac I, Warrender A, Sethi A, Jelinek R, Kass I
RgGuinier 2.1 nm
Dmax 6.5 nm
VolumePorod 41 nm3

SASDSZ5 – DNA binding domain of PALB2 (partner and localizer of BRCA2) in 500 mM NaCl

Partner and localizer of BRCA2 experimental SAS data
DNA binding domain of PALB2 (partner and localizer of BRCA2) in 500 mM NaCl Rg histogram
Sample: Partner and localizer of BRCA2 dimer, 46 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 500 mM NaCl, 0.5 mM TCEP, pH: 7.4
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Nov 11
The strand exchange domain of tumor suppressor PALB2 is intrinsically disordered and promotes oligomerization-dependent DNA compaction. iScience 27(12):111259 (2024)
Kyriukha Y, Watkins MB, Redington JM, Chintalapati N, Ganti A, Dastvan R, Uversky VN, Hopkins JB, Pozzi N, Korolev S
RgGuinier 5.5 nm
Dmax 25.4 nm
VolumePorod 171 nm3

SASDS26 – DNA binding domain of PALB2 (partner and localizer of BRCA2) in 160 mM NaCl

Partner and localizer of BRCA2 experimental SAS data
DNA binding domain of PALB2 (partner and localizer of BRCA2) in 160 mM NaCl Rg histogram
Sample: Partner and localizer of BRCA2 dimer, 46 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 160 mM NaCl, 0.5 mM TCEP, pH: 7.4
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Nov 11
The strand exchange domain of tumor suppressor PALB2 is intrinsically disordered and promotes oligomerization-dependent DNA compaction. iScience 27(12):111259 (2024)
Kyriukha Y, Watkins MB, Redington JM, Chintalapati N, Ganti A, Dastvan R, Uversky VN, Hopkins JB, Pozzi N, Korolev S
RgGuinier 4.7 nm
Dmax 21.3 nm
VolumePorod 197 nm3

SASDVQ7 – PABL2 (Partner and localizer of BRCA2) L24A mutant

Partner and localizer of BRCA2 (L24A) experimental SAS data
PABL2 (Partner and localizer of BRCA2) L24A mutant Rg histogram
Sample: Partner and localizer of BRCA2 (L24A) monomer, 23 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 160 mM NaCl, 0.5 mM TCEP pH 7.5, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Dec 9
The strand exchange domain of tumor suppressor PALB2 is intrinsically disordered and promotes oligomerization-dependent DNA compaction. iScience 27(12):111259 (2024)
Kyriukha Y, Watkins MB, Redington JM, Chintalapati N, Ganti A, Dastvan R, Uversky VN, Hopkins JB, Pozzi N, Korolev S
RgGuinier 5.2 nm
Dmax 21.6 nm
VolumePorod 127 nm3

SASDVR7 – PABL2 (Partner and localizer of BRCA2) incubated with dT50 (DNA)

Partner and localizer of BRCA2 experimental SAS data
Partner and localizer of BRCA2 Kratky plot
Sample: Partner and localizer of BRCA2 dimer, 46 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 160 mM NaCl, 0.5 mM TCEP pH 7.5, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Dec 9
The strand exchange domain of tumor suppressor PALB2 is intrinsically disordered and promotes oligomerization-dependent DNA compaction. iScience 27(12):111259 (2024)
Kyriukha Y, Watkins MB, Redington JM, Chintalapati N, Ganti A, Dastvan R, Uversky VN, Hopkins JB, Pozzi N, Korolev S
RgGuinier 4.6 nm
Dmax 17.6 nm
VolumePorod 236 nm3

SASDMV7 – The tetramer of splicing factor, proline- and glutamine-rich and Non-POU domain-containing octamer-binding protein (SFPQ214-598(R542C)/NONO53-312)

Splicing factor, proline- and glutamine-richNon-POU domain-containing octamer-binding protein experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Splicing factor, proline- and glutamine-rich dimer, 153 kDa Homo sapiens protein
Non-POU domain-containing octamer-binding protein dimer, 60 kDa Homo sapiens protein
Buffer: 20 mM Tris pH 7.5, 250 mM NaCl, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Jun 18
Structural plasticity of the coiled-coil interactions in human SFPQ. Nucleic Acids Res (2024)
Koning HJ, Lai JY, Marshall AC, Stroeher E, Monahan G, Pullakhandam A, Knott GJ, Ryan TM, Fox AH, Whitten A, Lee M, Bond CS
RgGuinier 5.5 nm
Dmax 20.4 nm
VolumePorod 304 nm3

SASDMW7 – Concentration dependent dimer-tetramer transition of splicing factor, proline- and glutamine-rich with Non-POU domain-containing octamer-binding protein (SFPQ276-565/NONO53-312)

Splicing factor, proline- and glutamine-rich (276-565)Non-POU domain-containing octamer-binding protein (53-312) experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Splicing factor, proline- and glutamine-rich (276-565) monomer, 34 kDa Homo sapiens protein
Non-POU domain-containing octamer-binding protein (53-312) monomer, 30 kDa Homo sapiens protein
Buffer: 20 mM Tris pH 7.5, 250 mM NaCl, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2015 Apr 28
Structural plasticity of the coiled-coil interactions in human SFPQ. Nucleic Acids Res (2024)
Koning HJ, Lai JY, Marshall AC, Stroeher E, Monahan G, Pullakhandam A, Knott GJ, Ryan TM, Fox AH, Whitten A, Lee M, Bond CS
RgGuinier 3.0 nm
Dmax 12.7 nm
VolumePorod 102 nm3

SASDMW8 – The disulphide-linked tetramer of splicing factor, proline- and glutamine-rich and Non-POU domain-containing octamer-binding protein (SFPQ276-598(R542C)/NONO53-312) at 0.78mg/ml

Non-POU domain-containing octamer-binding proteinSplicing factor, proline- and glutamine-rich SFPQ276-598(R542C)/NONO53-312 dimer) experimental SAS data
DAMMIF model
Sample: Non-POU domain-containing octamer-binding protein dimer, 60 kDa Homo sapiens protein
Splicing factor, proline- and glutamine-rich SFPQ276-598(R542C)/NONO53-312 dimer) dimer, 76 kDa Homo sapiens protein
Buffer: 20 mM Tris pH 7.5, 250 mM NaCl, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2015 Apr 28
Structural plasticity of the coiled-coil interactions in human SFPQ. Nucleic Acids Res (2024)
Koning HJ, Lai JY, Marshall AC, Stroeher E, Monahan G, Pullakhandam A, Knott GJ, Ryan TM, Fox AH, Whitten A, Lee M, Bond CS
RgGuinier 5.2 nm
Dmax 21.0 nm
VolumePorod 257 nm3

SASDVG6 – 2-nitroimidazole nitrohydrolase

2-nitroimidazole nitrohydrolase (T2I, G14D, K73R) experimental SAS data
REFMAC model
Sample: 2-nitroimidazole nitrohydrolase (T2I, G14D, K73R) hexamer, 258 kDa Mycobacterium sp. (strain … protein
Buffer: 25 mM Tris-HCl, 150 mM NaCl, 5% (v/v) glycerol, pH: 7.6
Experiment: SAXS data collected at BioSAXS, Australian Synchrotron on 2024 Aug 6
Structural insights into the enzymatic breakdown of azomycin-derived antibiotics by 2-nitroimdazole hydrolase (NnhA). Commun Biol 7(1):1676 (2024)
Ahmed FH, Liu JW, Royan S, Warden AC, Esquirol L, Pandey G, Newman J, Scott C, Peat TS
RgGuinier 4.6 nm
Dmax 12.6 nm
VolumePorod 363 nm3

SASDTA7 – RNase P RNA in the presence of 0.1 mM MgCl2

RNase P RNA experimental SAS data
RNase P RNA Kratky plot
Sample: RNase P RNA monomer, 135 kDa Geobacillus stearothermophilus RNA
Buffer: 100 mM NaCl, 0.1 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at Rigaku BIOSAXS-2000 [duplicate], Center for Cancer Research,National Cancer Institute on 2023 Nov 3
The conformational space of RNase P RNA in solution Nature (2024)
Lee Y, Degenhardt M, Skeparnias I, Degenhardt H, Bhandari Y, Yu P, Stagno J, Fan L, Zhang J, Wang Y
RgGuinier 6.1 nm
Dmax 21.8 nm
VolumePorod 813 nm3