Browse by DISSEMINATION: Published

SASDAM5 – Clostridium difficile bacteriophage 27 endolysin dimer C238R mutant, CD27L-C238R

Clostridium difficile bacteriophage 27 endolysin C238R mutant experimental SAS data
DAMMIF model
Sample: Clostridium difficile bacteriophage 27 endolysin C238R mutant dimer, 64 kDa Clostridioides difficile protein
Buffer: 20 mM HEPES 500 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Jun 21
The CD27L and CTP1L endolysins targeting Clostridia contain a built-in trigger and release factor. PLoS Pathog 10(7):e1004228 (2014)
Dunne M, Mertens HD, Garefalaki V, Jeffries CM, Thompson A, Lemke EA, Svergun DI, Mayer MJ, Narbad A, Meijers R
RgGuinier 4.2 nm
Dmax 14.7 nm
VolumePorod 92 nm3

SASDLH5 – Aggregation state of Ataxin-3 protein

Ataxin-3 experimental SAS data
DAMMIN model
Sample: Ataxin-3 monomer, 41 kDa Homo sapiens protein
Buffer: phosphate buffered saline (PBS), pH: 7.4
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Dec 3
Interactions of ataxin-3 with its molecular partners in the protein machinery that sorts protein aggregates to the aggresome. Int J Biochem Cell Biol 51:58-64 (2014)
Bonanomi M, Mazzucchelli S, D'Urzo A, Nardini M, Konarev PV, Invernizzi G, Svergun DI, Vanoni M, Regonesi ME, Tortora P
RgGuinier 6.9 nm
Dmax 25.0 nm
VolumePorod 425 nm3

SASDLJ5 – Aggregation state of tubulin protein

Tubulin alpha-1A chain experimental SAS data
DAMMIN model
Sample: Tubulin alpha-1A chain monomer, 50 kDa Homo sapiens protein
Buffer: phosphate buffered saline (PBS), pH: 7.4
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Dec 3
Interactions of ataxin-3 with its molecular partners in the protein machinery that sorts protein aggregates to the aggresome. Int J Biochem Cell Biol 51:58-64 (2014)
Bonanomi M, Mazzucchelli S, D'Urzo A, Nardini M, Konarev PV, Invernizzi G, Svergun DI, Vanoni M, Regonesi ME, Tortora P
RgGuinier 7.0 nm
Dmax 25.0 nm
VolumePorod 340 nm3

SASDLK5 – Aggregation state of ataxin-3-tubulin complex

Ataxin-3Tubulin alpha-1A chain experimental SAS data
DAMMIN model
Sample: Ataxin-3 monomer, 41 kDa Homo sapiens protein
Tubulin alpha-1A chain monomer, 50 kDa Homo sapiens protein
Buffer: phosphate buffered saline (PBS), pH: 7.4
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Dec 3
Interactions of ataxin-3 with its molecular partners in the protein machinery that sorts protein aggregates to the aggresome. Int J Biochem Cell Biol 51:58-64 (2014)
Bonanomi M, Mazzucchelli S, D'Urzo A, Nardini M, Konarev PV, Invernizzi G, Svergun DI, Vanoni M, Regonesi ME, Tortora P
RgGuinier 8.4 nm
Dmax 30.0 nm
VolumePorod 900 nm3

SASDLW4 – Human Protein S100-A4

Protein S100-A4 experimental SAS data
EOM/RANCH model
Sample: Protein S100-A4 dimer, 23 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 20 mM NaCl, 0.1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2013 Mar 2
The C-Terminal Random Coil Region Tunes the Ca2+-Binding Affinity of S100A4 through Conformational Activation PLoS ONE 9(5):e97654 (2014)
Duelli A, Kiss B, Lundholm I, Bodor A, Petoukhov M, Svergun D, Nyitray L, Katona G, Csernoch L
RgGuinier 2.1 nm
Dmax 7.0 nm
VolumePorod 48 nm3

SASDDF6 – Myelin basic protein

Myelin basic protein experimental SAS data
Myelin basic protein Rg histogram
Sample: Myelin basic protein monomer, 18 kDa Bos taurus protein
Buffer: 20 mM NaH2PO4/ Na2HPO4, 99.9% D2O, pH: 4.8
Experiment: SAXS data collected at BM29, ESRF on 2013 Feb 21
Internal nanosecond dynamics in the intrinsically disordered myelin basic protein. J Am Chem Soc 136(19):6987-94 (2014)
Stadler AM, Stingaciu L, Radulescu A, Holderer O, Monkenbusch M, Biehl R, Richter D
RgGuinier 3.3 nm
Dmax 11.1 nm

SASDAP4 – Chitinase 60 from Moritella marina

Chitinase 60 experimental SAS data
Chitinase 60 from Moritella marina Rg histogram
Sample: Chitinase 60 monomer, 61 kDa Moritella marina protein
Buffer: 20 mM Tris 200 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Mar 18
Crystal structures of substrate-bound chitinase from the psychrophilic bacterium Moritella marina and its structure in solution. Acta Crystallogr D Biol Crystallogr 70(Pt 3):676-84 (2014)
Malecki PH, Vorgias CE, Petoukhov MV, Svergun DI, Rypniewski W
RgGuinier 3.2 nm
Dmax 11.3 nm
VolumePorod 75 nm3

SASDBF4 – Wild-type LytA (N-His6) with choline

Lytic Amidase with choline experimental SAS data
SASREF model
Sample: Lytic Amidase with choline dimer, 75 kDa Streptococcus pneumoniae protein
Buffer: 20 mM Tris 150 mM NaCl 5 mM choline chloride 1 µM ZnCl2, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Jul 2
Structural and functional insights into peptidoglycan access for the lytic amidase LytA of Streptococcus pneumoniae. MBio 5(1):e01120-13 (2014)
Mellroth P, Sandalova T, Kikhney A, Vilaplana F, Hesek D, Lee M, Mobashery S, Normark S, Svergun D, Henriques-Normark B, Achour A
RgGuinier 4.9 nm
Dmax 15.0 nm
VolumePorod 115 nm3

SASDBG4 – Prophage LytA with choline

Prophage Lytic Amidase with choline experimental SAS data
SASREF model
Sample: Prophage Lytic Amidase with choline dimer, 73 kDa Streptococcus pneumoniae protein
Buffer: 20 mM Tris 150 mM NaCl 5 mM choline chloride 1 µM ZnCl2, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Jul 2
Structural and functional insights into peptidoglycan access for the lytic amidase LytA of Streptococcus pneumoniae. MBio 5(1):e01120-13 (2014)
Mellroth P, Sandalova T, Kikhney A, Vilaplana F, Hesek D, Lee M, Mobashery S, Normark S, Svergun D, Henriques-Normark B, Achour A
RgGuinier 5.9 nm
Dmax 17.0 nm
VolumePorod 130 nm3

SASDBH4 – Wild-type LytA (N-His6) without choline

Lytic Amidase without choline experimental SAS data
SASREF model
Sample: Lytic Amidase without choline monomer, 37 kDa Streptococcus pneumoniae protein
Buffer: 20 mM Tris 150 mM NaCl 1 µM ZnCl2, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Jul 3
Structural and functional insights into peptidoglycan access for the lytic amidase LytA of Streptococcus pneumoniae. MBio 5(1):e01120-13 (2014)
Mellroth P, Sandalova T, Kikhney A, Vilaplana F, Hesek D, Lee M, Mobashery S, Normark S, Svergun D, Henriques-Normark B, Achour A
RgGuinier 3.4 nm
Dmax 12.5 nm
VolumePorod 56 nm3