Small Angle Scattering Biological Data Bank SASBDB

Browse by MODEL: Ab initio only

SASDEV9 – Cytohesin-2; ARF nucleotide-binding site opener, ARNO truncation mutant

Cytohesin-2; ARNO truncation mutant experimental SAS data

Sample:	Cytohesin-2; ARNO truncation mutant monomer, 40 kDa Homo sapiens protein
Buffer:	300 mM NaCl, 2 mM 2-mercaptoethanol and 30 mM Tris-HCl, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2015 Nov 25

Structural Organization and Dynamics of Homodimeric Cytohesin Family Arf GTPase Exchange Factors in Solution and on Membranes. Structure (2019)
Das S, Malaby AW, Nawrotek A, Zhang W, Zeghouf M, Maslen S, Skehel M, Chakravarthy S, Irving TC, Bilsel O, Cherfils J, Lambright DG

R_g^Guinier	2.7	nm
D_max	9.9	nm
Volume^Porod	63	nm³

SASDEW9 – Cytohesin-2; ARF nucleotide-binding site opener, ARNO

Cytohesin-2 ARF nucleotide-binding site opener experimental SAS data

Sample:	Cytohesin-2 ARF nucleotide-binding site opener dimer, 93 kDa Homo sapiens protein
Buffer:	300 mM NaCl, 2 mM 2-mercaptoethanol and 30 mM Tris-HCl, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2016 Jun 23

R_g^Guinier	4.8	nm
D_max	19.7	nm
Volume^Porod	145	nm³

SASDG64 – Autoinhibited dimer of truncated 6xHis Cytohesin-3 (Grp1, amino acids 14-399) with Inositol 1,3,4,5-tetrakis phosphate (DAMMIF and GASBOR models)

Sample:	Cytohesin-3 dimer, 93 kDa Mus musculus protein
Buffer:	20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 15

R_g^Guinier	5.5	nm
D_max	26.0	nm
Volume^Porod	194	nm³

SASDG74 – Active dimer of truncated 6xHis Cytohesin-3 (Grp1, amino acids 14-390) with Inositol 1,3,4,5-tetrakis phosphate (DAMMIF and GASBOR models)

Sample:	Cytohesin-3 dimer, 90 kDa Mus musculus protein
Buffer:	20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 15

R_g^Guinier	5.3	nm
D_max	25.7	nm
Volume^Porod	168	nm³

SASDG26 – Wild type 4-hydroxy-tetrahydrodipicolinate synthase

4-hydroxy-tetrahydrodipicolinate synthase experimental SAS data

Sample:	4-hydroxy-tetrahydrodipicolinate synthase tetramer, 131 kDa Campylobacter jejuni protein
Buffer:	20 mM Tris-HCl, 150 mM NaCl, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2017 Aug 2

Asparagine-84, a regulatory allosteric site residue, helps maintain the quaternary structure of Campylobacter jejuni dihydrodipicolinate synthase. J Struct Biol :107409 (2019)
Majdi Yazdi M, Saran S, Mrozowich T, Lehnert C, Patel TR, Sanders DAR, Palmer DRJ

R_g^Guinier	3.4	nm
D_max	9.0	nm
Volume^Porod	188	nm³

SASDG36 – 4-hydroxy-tetrahydrodipicolinate synthase (N84D mutant)

4-hydroxy-tetrahydrodipicolinate synthase (N84D mutant) experimental SAS data

Sample:	4-hydroxy-tetrahydrodipicolinate synthase (N84D mutant) dimer, 65 kDa Campylobacter jejuni protein
Buffer:	20 mM Tris-HCl, 150 mM NaCl, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2017 Aug 2

R_g^Guinier	3.1	nm
D_max	9.5	nm
Volume^Porod	108	nm³

SASDG46 – 4-hydroxy-tetrahydrodipicolinate synthase (N84A mutant)

4-hydroxy-tetrahydrodipicolinate synthase (N84A mutant) experimental SAS data

Sample:	4-hydroxy-tetrahydrodipicolinate synthase (N84A mutant), 33 kDa Campylobacter jejuni protein
Buffer:	20 mM Tris-HCl, 150 mM NaCl, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2017 Aug 2

R_g^Guinier	3.3	nm
D_max	8.9	nm
Volume^Porod	163	nm³

SASDFC5 – Enhanced disease susceptibility 1 (EDS1)

Enhanced disease susceptibility experimental SAS data

Sample:	Enhanced disease susceptibility monomer, 72 kDa Arabidopsis thaliana protein
Buffer:	50 mM NaCl, 50 mM HEPES, 1% glyercol, 1 mM DTT, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2015 May 19

Arabidopsis immunity regulator EDS1 in a PAD4/SAG101-unbound form is a monomer with an inherently inactive conformation. J Struct Biol :107390 (2019)
Voss M, Toelzer C, Bhandari DD, Parker JE, Niefind K

R_g^Guinier	3.1	nm
D_max	10.5	nm
Volume^Porod	92	nm³

SASDFL5 – Plasmodium falciparum Hsp70/Hsp90 organizing protein, Hop

Sample:	STI1-like protein dimer, 136 kDa Plasmodium falciparum protein
Buffer:	25 mM Tris-HCl, 100 mM NaCl, 1 mM EDTA, 1 mM β-mercaptoethanol, pH: 8
Experiment:	SAXS data collected at SAXS1 Beamline, Brazilian Synchrotron Light Laboratory on 2016 Aug 3

Structural studies of the Hsp70/Hsp90 organizing protein of Plasmodium falciparum and its modulation of Hsp70 and Hsp90 ATPase activities. Biochim Biophys Acta Proteins Proteom :140282 (2019)
Silva NSM, Bertolino-Reis DE, Dores-Silva PR, Anneta FB, Seraphim TV, Barbosa LRS, Borges JC

R_g^Guinier	6.3	nm
D_max	24.0	nm
Volume^Porod	557	nm³

SASDF27 – Brain and Muscle ARNT-Like 1 from Mus musculus (D530-L625), monomer, trans-conformation locking mutation P624A

Aryl hydrocarbon receptor nuclear translocator-like protein 1 experimental SAS data

Sample:	Aryl hydrocarbon receptor nuclear translocator-like protein 1 monomer, 10 kDa Mus musculus protein
Buffer:	25 mM Hepes, 150 NaCl, 1 mM DTT, 5% Glycerol, pH: 7.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 May 27

Structural and mechanistic insights into the interaction of the circadian transcription factor BMAL1 with the KIX domain of the CREB-binding protein. J Biol Chem (2019)
Garg A, Orru R, Ye W, Distler U, Chojnacki JE, Köhn M, Tenzer S, Sönnichsen C, Wolf E

R_g^Guinier	2.8	nm
D_max	11.0	nm

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