Browse by MODEL: Ensemble

SASDJE6 – Metallothionein (MT) 2 protein from the plant Cicer arietinum

Metallothionein-like protein 2 experimental SAS data
Metallothionein (MT) 2 protein from the plant Cicer arietinum Rg histogram
Sample: Metallothionein-like protein 2 monomer, 8 kDa Cicer arietinum protein
Buffer: 10 mM Tris, 50 mM NaCl, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Aug 8
Structural Characterization of Plant Metallothionein
Dima Molodenskiy
RgGuinier 1.9 nm
Dmax 7.3 nm
VolumePorod 5 nm3

SASDJF6 – Apo-full-length protein titrated with Cd(II) ions

Metallothionein-like protein 2 experimental SAS data
Apo-full-length protein titrated with Cd(II) ions Rg histogram
Sample: Metallothionein-like protein 2 monomer, 8 kDa Cicer arietinum protein
Buffer: 10 mM Tris, 50 mM NaCl + 1mM TCEP (apoMT), pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Aug 8
Structural Characterization of Plant Metallothionein
Dima Molodenskiy
RgGuinier 2.6 nm
Dmax 10.0 nm
VolumePorod 13 nm3

SASDJG6 – The separated apo-linker region peptide

The separated apo-linker region peptide from the plant Cicer arietinum experimental SAS data
The separated apo-linker region peptide Rg histogram
Sample: The separated apo-linker region peptide from the plant Cicer arietinum monomer, 4 kDa Cicer arietinum protein
Buffer: 10 mM Tris, 50 mM NaCl, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Aug 8
Structural Characterization of Plant Metallothionein
Dima Molodenskiy
RgGuinier 1.4 nm
Dmax 6.5 nm
VolumePorod 1 nm3

SASDJH6 – The CxxC-linker-CxxC construct binding 1 Cd(II) ion in a CdCys4 site

A CxxC-linker-CxxC construct binding 1 Cd(II) ion in a CdCys4 site experimental SAS data
The CxxC-linker-CxxC construct binding 1 Cd(II) ion in a CdCys4 site Rg histogram
Sample: A CxxC-linker-CxxC construct binding 1 Cd(II) ion in a CdCys4 site monomer, 5 kDa Cicer arietinum protein
Buffer: 10 mM Tris, 50 mM NaCl, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Aug 8
Structural Characterization of Plant Metallothionein
Dima Molodenskiy
RgGuinier 1.9 nm
Dmax 6.3 nm

SASDKT4 – Teneurin-4 dimer wildtype in SEC buffer with calcium (20 mM HEPES, 150 mM NaCl, 2 mM CaCl2)

Teneurin-4 experimental SAS data
Teneurin-4 dimer wildtype in SEC buffer with calcium (20 mM HEPES, 150 mM NaCl, 2 mM CaCl2) Rg histogram
Sample: Teneurin-4 dimer, 537 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH: 7.8
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
Teneurin4 dimer structures reveal a calcium‐stabilized compact conformation supporting homomeric trans‐interactions The EMBO Journal (2022)
Meijer D, Frias C, Beugelink J, Deurloo Y, Janssen B
RgGuinier 7.2 nm
Dmax 29.5 nm
VolumePorod 1270 nm3

SASDKU4 – Teneurin-4 dimer wildtype in SEC buffer with EDTA (20 mM HEPES, 150 mM NaCl, 2 mM EDTA)

Teneurin-4 experimental SAS data
Teneurin-4 dimer wildtype in SEC buffer with EDTA (20 mM HEPES, 150 mM NaCl, 2 mM EDTA) Rg histogram
Sample: Teneurin-4 dimer, 537 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM EDTA, pH: 7.8
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
Teneurin4 dimer structures reveal a calcium‐stabilized compact conformation supporting homomeric trans‐interactions The EMBO Journal (2022)
Meijer D, Frias C, Beugelink J, Deurloo Y, Janssen B
RgGuinier 9.5 nm
Dmax 40.0 nm
VolumePorod 1370 nm3

SASDMG4 – Arrestin-3 ERK2 fusion, monomer (Arrestin-3 fused to extracellular signal-regulated kinase 2)

Arrestin-3 fused to extracellular signal-regulated kinase 2 experimental SAS data
Arrestin-3 ERK2 fusion, monomer (Arrestin-3 fused to extracellular signal-regulated kinase 2) Rg histogram
Sample: Arrestin-3 fused to extracellular signal-regulated kinase 2 monomer, 89 kDa Bos taurus/Rattus norvegicus protein
Buffer: 20 mM MOPS pH 7.5, 150 mM NaCl, 1 mM TCEP, and 5% glycerol, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Mar 23
The two non-visual arrestins engage ERK2 differently. J Mol Biol :167465 (2022)
Perry-Hauser NA, Bennett Hopkins J, Zhuo Y, Zheng C, Perez I, Schultz KM, Vishnivetskiy SA, Kaya AI, Sharma P, Dalby KN, Young Chung K, Klug CS, Gurevich VV, Iverson TM
RgGuinier 3.6 nm
Dmax 14.0 nm

SASDLL9 – Nipah henipavirus W protein in 1 M urea, 5 mM DTT

Protein W experimental SAS data
Nipah henipavirus W protein in 1 M urea, 5 mM DTT Rg histogram
Sample: Protein W monomer, 53 kDa Nipah henipavirus protein
Buffer: 20 mM HEPES, 150 mM NaCl, 1 M urea, 5 mM DTT, pH: 7
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Jun 12
Experimental Evidence of Intrinsic Disorder and Amyloid Formation by the Henipavirus W Proteins International Journal of Molecular Sciences 23(2):923 (2022)
Pesce G, Gondelaud F, Ptchelkine D, Nilsson J, Bignon C, Cartalas J, Fourquet P, Longhi S
RgGuinier 7.1 nm
Dmax 24.5 nm
VolumePorod 327 nm3

SASDLK9 – Hendra virus W protein in 1 M urea, 5 mM DTT

Protein W experimental SAS data
Hendra virus W protein in 1 M urea, 5 mM DTT Rg histogram
Sample: Protein W monomer, 53 kDa Hendra virus (isolate … protein
Buffer: 20 mM HEPES, 150 mM NaCl, 1 M urea, 5 mM DTT, pH: 7
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Jun 12
Experimental Evidence of Intrinsic Disorder and Amyloid Formation by the Henipavirus W Proteins International Journal of Molecular Sciences 23(2):923 (2022)
Pesce G, Gondelaud F, Ptchelkine D, Nilsson J, Bignon C, Cartalas J, Fourquet P, Longhi S
RgGuinier 7.2 nm
Dmax 24.0 nm
VolumePorod 338 nm3

SASDMX3 – Anti-CD20 IgG antibody

Anti-CD20 IgG antibody (Rituximab heavy chain chimeric)Anti-CD20 IgG antibody (Rituximab light chain chimeric) experimental SAS data
Sample: Anti-CD20 IgG antibody (Rituximab heavy chain chimeric) dimer, 98 kDa Homo sapiens protein
Anti-CD20 IgG antibody (Rituximab light chain chimeric) dimer, 46 kDa Homo sapiens protein
Buffer: 100 mM HEPES, pH: 7.7
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Sep 20
Structural Characterization of the Full-Length Anti-CD20 Antibody Rituximab. Front Mol Biosci 9:823174 (2022)
Belviso BD, Mangiatordi GF, Alberga D, Mangini V, Carrozzini B, Caliandro R
RgGuinier 5.2 nm
Dmax 19.7 nm