Browse by MODEL: Ensemble

SASDP63 – Accumulation-association protein (Aap) Brpt5.5 2xH85A monomer

Accumulation associated protein (mutant) experimental SAS data
Accumulation-association protein (Aap) Brpt5.5 2xH85A monomer Rg histogram
Sample: Accumulation associated protein (mutant) monomer, 78 kDa Staphylococcus epidermidis (strain … protein
Buffer: 50 mM MOPS, 50 mM NaCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Mar 1
Solution structural studies of pre-amyloid oligomer states of the biofilm protein Aap. J Mol Biol :167708 (2022)
Yarawsky AE, Hopkins JB, Chatzimagas L, Hub JS, Herr AB
RgGuinier 14.4 nm
Dmax 56.6 nm

SASDMS5 – Glutathione S-transferase/RNA recognition motif (RRM)-containing protein 4 fusion

RNA recognition motif (RRM)-containing protein 4 experimental SAS data
Glutathione S-transferase/RNA recognition motif (RRM)-containing protein 4 fusion Rg histogram
Sample: RNA recognition motif (RRM)-containing protein 4 monomer, 111 kDa Ustilago maydis protein
Buffer: 20 mM Hepes, 200 mM NaCl, 1 mM βME, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2021 Apr 30
A MademoiseLLE domain binding platform links the key RNA transporter to endosomes PLOS Genetics 18(6):e1010269 (2022)
Devan S, Schott-Verdugo S, Müntjes K, Bismar L, Reiners J, Hachani E, Schmitt L, Höppner A, Smits S, Gohlke H, Feldbrügge M, Mitchell A
RgGuinier 8.8 nm
Dmax 30.7 nm
VolumePorod 587 nm3

SASDMT5 – N-terminal histidine tagged RNA recognition motif (RRM)-containing protein 4 NT4

RNA recognition motif (RRM)-containing protein 4 NT4 experimental SAS data
N-terminal histidine tagged RNA recognition motif (RRM)-containing protein 4 NT4 Rg histogram
Sample: RNA recognition motif (RRM)-containing protein 4 NT4 monomer, 40 kDa Ustilago maydis protein
Buffer: 20 mM Hepes, 200 mM NaCl, 1 mM βME, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2021 Apr 30
A MademoiseLLE domain binding platform links the key RNA transporter to endosomes PLOS Genetics 18(6):e1010269 (2022)
Devan S, Schott-Verdugo S, Müntjes K, Bismar L, Reiners J, Hachani E, Schmitt L, Höppner A, Smits S, Gohlke H, Feldbrügge M, Mitchell A
RgGuinier 5.6 nm
Dmax 18.5 nm
VolumePorod 123 nm3

SASDL32 – Transcription elongation factor SPT6

Transcription elongation factor SPT6 experimental SAS data
Transcription elongation factor SPT6 Rg histogram
Sample: Transcription elongation factor SPT6 monomer, 145 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 25 mM NaPi; 150mM NaCl; 0.5 mM EDTA; 5% glycerol; 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Oct 22
Cooperation between intrinsically disordered and ordered regions of Spt6 regulates nucleosome and Pol II CTD binding, and nucleosome assembly. Nucleic Acids Res (2022)
Kasiliauskaite A, Kubicek K, Klumpler T, Zanova M, Zapletal D, Koutna E, Novacek J, Stefl R
RgGuinier 4.5 nm
Dmax 12.0 nm
VolumePorod 249 nm3

SASDL22 – Transcription elongation factor SPT6 - ΔtSH2 variant

Transcription elongation factor SPT6 - ΔtSH2 variant experimental SAS data
Transcription elongation factor SPT6 - ΔtSH2 variant Rg histogram
Sample: Transcription elongation factor SPT6 - ΔtSH2 variant monomer, 122 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 25 mM NaPi; 150mM NaCl; 0.5 mM EDTA; 5% glycerol; 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Oct 22
Cooperation between intrinsically disordered and ordered regions of Spt6 regulates nucleosome and Pol II CTD binding, and nucleosome assembly. Nucleic Acids Res (2022)
Kasiliauskaite A, Kubicek K, Klumpler T, Zanova M, Zapletal D, Koutna E, Novacek J, Stefl R
RgGuinier 4.1 nm
Dmax 13.9 nm
VolumePorod 245 nm3

SASDKZ9 – Transcription elongation factor SPT6 - ΔN Spt6

Transcription elongation factor SPT6 - ΔN Spt6 variant experimental SAS data
Transcription elongation factor SPT6 - ΔN Spt6 Rg histogram
Sample: Transcription elongation factor SPT6 - ΔN Spt6 variant monomer, 132 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 25 mM Hepes; 150 NaCl; 0.5 mM EDTA; 5% glycerol; 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3
Cooperation between intrinsically disordered and ordered regions of Spt6 regulates nucleosome and Pol II CTD binding, and nucleosome assembly. Nucleic Acids Res (2022)
Kasiliauskaite A, Kubicek K, Klumpler T, Zanova M, Zapletal D, Koutna E, Novacek J, Stefl R
RgGuinier 4.7 nm
Dmax 14.4 nm
VolumePorod 288 nm3

SASDLM8 – Human apo Nocturnin - Extended

Nocturnin experimental SAS data
Human apo Nocturnin - Extended Rg histogram
Sample: Nocturnin monomer, 41 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM KCl, 10% glycerol, 5 mM MgCl2, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Feb 7
The Disordered Amino Terminus of the Circadian Enzyme Nocturnin Modulates Its NADP(H) Phosphatase Activity by Changing Protein Dynamics. Biochemistry (2022)
Wickramaratne AC, Li L, Hopkins JB, Joachimiak LA, Green CB
RgGuinier 2.9 nm
Dmax 12.3 nm
VolumePorod 85 nm3

SASDLN8 – Human apo Nocturnin - Compact

Nocturnin experimental SAS data
Human apo Nocturnin - Compact Rg histogram
Sample: Nocturnin monomer, 41 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM KCl, 10% glycerol, 5 mM MgCl2, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Feb 7
The Disordered Amino Terminus of the Circadian Enzyme Nocturnin Modulates Its NADP(H) Phosphatase Activity by Changing Protein Dynamics. Biochemistry (2022)
Wickramaratne AC, Li L, Hopkins JB, Joachimiak LA, Green CB
RgGuinier 2.4 nm
Dmax 9.6 nm
VolumePorod 76 nm3

SASDLQ8 – Human apo Nocturnin - Deletion construct (Δ107-120) - Compact

Nocturnin - Deletion construct - Δ107-120 experimental SAS data
Human apo Nocturnin - Deletion construct (Δ107-120) - Compact Rg histogram
Sample: Nocturnin - Deletion construct - Δ107-120 monomer, 40 kDa protein
Buffer: 50 mM HEPES, 150 mM KCl, 10% glycerol, 5 mM MgCl2, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Feb 7
The Disordered Amino Terminus of the Circadian Enzyme Nocturnin Modulates Its NADP(H) Phosphatase Activity by Changing Protein Dynamics. Biochemistry (2022)
Wickramaratne AC, Li L, Hopkins JB, Joachimiak LA, Green CB
RgGuinier 2.5 nm
Dmax 10.0 nm
VolumePorod 80 nm3

SASDLE9 – Suppressor of Copper Sensitivity C protein from Caulobacter crescentus

Thioredoxin domain-containing protein experimental SAS data
Suppressor of Copper Sensitivity C protein from Caulobacter crescentus Rg histogram
Sample: Thioredoxin domain-containing protein trimer, 73 kDa Caulobacter vibrioides (strain … protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Aug 21
The suppressor of copper sensitivity protein C from Caulobacter crescentus is a trimeric disulfide isomerase that binds copper(I) with subpicomolar affinity Acta Crystallographica Section D Structural Biology 78(3):337-352 (2022)
Petit G, Hong Y, Djoko K, Whitten A, Furlong E, McCoy A, Gulbis J, Totsika M, Martin J, Halili M
RgGuinier 3.9 nm
Dmax 12.0 nm
VolumePorod 97 nm3