Browse by MODEL: Ensemble

SASDBD6 – Single stranded poly-deoxythymidine DNA (30mer, dT30)

Poly-deoxythymidine (30mer) experimental SAS data
Single stranded poly-deoxythymidine DNA (30mer, dT30) Rg histogram
Sample: Poly-deoxythymidine (30mer) monomer, 9 kDa DNA
Buffer: 1 mM Na-MOPS, 20 mM NaCl, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Apr 1
Visualizing single-stranded nucleic acids in solution. Nucleic Acids Res 45(9):e66 (2017)
Plumridge A, Meisburger SP, Pollack L
RgGuinier 3.0 nm
Dmax 10.7 nm

SASDBE6 – Single stranded poly-deoxyadenosine DNA (30mer, dA30)

Poly-deoxyadenosine (30mer) experimental SAS data
Single stranded poly-deoxyadenosine DNA (30mer, dA30) Rg histogram
Sample: Poly-deoxyadenosine (30mer) monomer, 9 kDa DNA
Buffer: 1 mM Na-MOPS, 20 mM NaCl, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Apr 1
Visualizing single-stranded nucleic acids in solution. Nucleic Acids Res 45(9):e66 (2017)
Plumridge A, Meisburger SP, Pollack L
RgGuinier 2.7 nm
Dmax 9.5 nm

SASDBE7 – ESX-5 type VII secretion system protein EccC5

ESX-5 type VII secretion system protein EccC5 experimental SAS data
ESX-5 type VII secretion system protein EccC5 Rg histogram
Sample: ESX-5 type VII secretion system protein EccC5 monomer, 142 kDa Mycobacterium xenopi RIVM700367 protein
Buffer: 20 mM Tris 200 mM NaCl 5%(v/v) Glycerol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Jul 15
Structure of the mycobacterial ESX-5 type VII secretion system membrane complex by single-particle analysis. Nat Microbiol 2:17047 (2017)
Beckham KS, Ciccarelli L, Bunduc CM, Mertens HD, Ummels R, Lugmayr W, Mayr J, Rettel M, Savitski MM, Svergun DI, Bitter W, Wilmanns M, Marlovits TC, Parret AH, Houben EN
RgGuinier 6.2 nm
Dmax 23.0 nm
VolumePorod 255 nm3

SASDBS6 – Apo form of full length ObgE from E.coli (ObgE_FL)

GTPase ObgE/CgtA experimental SAS data
Apo form of full length ObgE from E.coli (ObgE_FL) Rg histogram
Sample: GTPase ObgE/CgtA monomer, 44 kDa Escherichia coli protein
Buffer: 20 mM Hepes , 300 mM NaCl, 250 mM imidazole, 5 mM MgCl2, 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2015 Jun 17
Structural and biochemical analysis of Escherichia coli ObgE, a central regulator of bacterial persistence. J Biol Chem 292(14):5871-5883 (2017)
Gkekas S, Singh RK, Shkumatov AV, Messens J, Fauvart M, Verstraeten N, Michiels J, Versées W
RgGuinier 3.7 nm
Dmax 18.1 nm
VolumePorod 102 nm3

SASDBU6 – Apo form of the C-terminal deletion mutant of ObgE from E.coli (ObgE_340)

GTPase ObgE/CgtA experimental SAS data
Apo form of the C-terminal deletion mutant of ObgE from E.coli (ObgE_340) Rg histogram
Sample: GTPase ObgE/CgtA monomer, 39 kDa Escherichia coli protein
Buffer: 20 mM Hepes , 300 mM NaCl, 250 mM imidazole, 5 mM MgCl2, 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2015 Mar 12
Structural and biochemical analysis of Escherichia coli ObgE, a central regulator of bacterial persistence. J Biol Chem 292(14):5871-5883 (2017)
Gkekas S, Singh RK, Shkumatov AV, Messens J, Fauvart M, Verstraeten N, Michiels J, Versées W
RgGuinier 3.1 nm
Dmax 11.6 nm
VolumePorod 70 nm3

SASDB47 – Full length tetrameric FKBP39 protein from Drosophila melanogaster

39 kDa FK506-binding nuclear protein experimental SAS data
Full length tetrameric FKBP39 protein from Drosophila melanogaster Rg histogram
Sample: 39 kDa FK506-binding nuclear protein tetramer, 163 kDa Drosophila melanogaster protein
Buffer: 50 mM Na2HPO4 150 mM NaCl, pH: 7
Experiment: SAXS data collected at B21, Diamond Light Source on 2014 Nov 14
Nucleoplasmin-like domain of FKBP39 from Drosophila melanogaster forms a tetramer with partly disordered tentacle-like C-terminal segments. Sci Rep 7:40405 (2017)
Kozłowska M, Tarczewska A, Jakób M, Bystranowska D, Taube M, Kozak M, Czarnocki-Cieciura M, Dziembowski A, Orłowski M, Tkocz K, Ożyhar A
RgGuinier 5.7 nm
Dmax 22.0 nm
VolumePorod 275 nm3

SASDDD9 – Cardiac myosin binding protein C: tri-helix bundle from the motif with C2 domain

cardiac myosin binding protein C: tri-helix bundle-C2 experimental SAS data
Cardiac myosin binding protein C: tri-helix bundle from the motif with C2 domain Rg histogram
Sample: Cardiac myosin binding protein C: tri-helix bundle-C2 monomer, 15 kDa human sequence obtained … protein
Buffer: 150 mM NaCl, 10 mM MES, 2 mM TCEP, 1 mM NaN3 at 4°C, pH: 6.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2015 Apr 18
A Highly Conserved Yet Flexible Linker Is Part of a Polymorphic Protein-Binding Domain in Myosin-Binding Protein C. Structure 24(11):2000-2007 (2016)
Michie KA, Kwan AH, Tung CS, Guss JM, Trewhella J
RgGuinier 1.9 nm
Dmax 8.0 nm
VolumePorod 20 nm3

SASDBE4 – Plakin domain of Human desmoplakin

Plakin domain of Human Desmoplakin experimental SAS data
Plakin domain of Human desmoplakin Rg histogram
Sample: Plakin domain of Human Desmoplakin monomer, 99 kDa Homo sapiens protein
Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Sep 25
The Structure of the Plakin Domain of Plectin Reveals an Extended Rod-like Shape. J Biol Chem 291(36):18643-62 (2016)
Ortega E, Manso JA, Buey RM, Carballido AM, Carabias A, Sonnenberg A, de Pereda JM
RgGuinier 7.5 nm
Dmax 35.0 nm
VolumePorod 136 nm3

SASDAC6 – PsrP functional binding region

Functional binding region (187-385) of the pneumococcal serine-rich repeat protein experimental SAS data
PsrP functional binding region Rg histogram
Sample: Functional binding region (187-385) of the pneumococcal serine-rich repeat protein monomer, 22 kDa Streptococcus pneumoniae protein
Buffer: PBS 5 % Glycerol, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2013 Feb 27
The BR domain of PsrP interacts with extracellular DNA to promote bacterial aggregation; structural insights into pneumococcal biofilm formation. Sci Rep 6:32371 (2016)
Schulte T, Mikaelsson C, Beaussart A, Kikhney A, Deshmukh M, Wolniak S, Pathak A, Ebel C, Löfling J, Fogolari F, Henriques-Normark B, Dufrêne YF, Svergun D, Nygren PÅ, Achour A
RgGuinier 2.0 nm
Dmax 7.8 nm
VolumePorod 38 nm3

SASDAE6 – PsrP functional binding region

Functional binding region (120-395) of the pneumococcal serine-rich repeat protein experimental SAS data
PsrP functional binding region Rg histogram
Sample: Functional binding region (120-395) of the pneumococcal serine-rich repeat protein monomer, 30 kDa Streptococcus pneumoniae protein
Buffer: PBS 5 % Glycerol, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2013 Feb 27
The BR domain of PsrP interacts with extracellular DNA to promote bacterial aggregation; structural insights into pneumococcal biofilm formation. Sci Rep 6:32371 (2016)
Schulte T, Mikaelsson C, Beaussart A, Kikhney A, Deshmukh M, Wolniak S, Pathak A, Ebel C, Löfling J, Fogolari F, Henriques-Normark B, Dufrêne YF, Svergun D, Nygren PÅ, Achour A
RgGuinier 2.9 nm
Dmax 12.5 nm
VolumePorod 41 nm3