Small Angle Scattering Biological Data Bank SASBDB

Browse by MODEL: Hybrid

SASDFK9 – Poly-uridine rU30 in 100 mM NaCl

Sample:	Poly-uridine monomer, 9 kDa RNA
Buffer:	1 mM Na-MOPS, 100 mM NaCl, 20 µM EDTA, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25

Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
Plumridge A, Andresen K, Pollack L

R_g^Guinier	2.7	nm
D_max	12.7	nm
Volume^Porod	22	nm³

SASDFL9 – Poly-uridine rU30 in 200 mM NaCl

Sample:	Poly-uridine monomer, 9 kDa RNA
Buffer:	1 mM Na-MOPS, 200 mM NaCl, 20 µM EDTA, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25

Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
Plumridge A, Andresen K, Pollack L

R_g^Guinier	2.5	nm
D_max	11.0	nm
Volume^Porod	15	nm³

SASDFP9 – Poly-uridine rU30 in 1 mM MgCl2

Sample:	Poly-uridine monomer, 9 kDa RNA
Buffer:	1 mM Na-MOPS, 20 mM NaCl, 1 mM MgCl2, 20 µM EDTA, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 24

Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
Plumridge A, Andresen K, Pollack L

R_g^Guinier	2.7	nm
D_max	11.0	nm
Volume^Porod	17	nm³

SASDFQ9 – Poly-uridine rU30 in 2 mM MgCl2

Sample:	Poly-uridine monomer, 9 kDa RNA
Buffer:	1 mM Na-MOPS, 20 mM NaCl, 2 mM MgCl2, 20 µM EDTA, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 24

Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
Plumridge A, Andresen K, Pollack L

R_g^Guinier	2.6	nm
D_max	10.5	nm
Volume^Porod	16	nm³

SASDD43 – Mycobacterial suicide toxin (MbsT)

Mycobacterial cidal toxin experimental SAS data

Sample:	Mycobacterial cidal toxin monomer, 20 kDa Mycobacterium tuberculosis H37Rv protein
Buffer:	30 mM Tris-HCl, 200 mM NaCl, 10% glycerol, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 May 2

MbsTA
Diana Freire

R_g^Guinier	1.8	nm
D_max	5.6	nm
Volume^Porod	30	nm³

SASDFL8 – GON7, the fifth subunit of human KEOPS, bound to LAGE3-OSGEP (EKC/KEOPS complex subunit:Probable tRNA N6-adenosine threonylcarbamoyltransferase)

EKC/KEOPS complex subunit GON7EKC/KEOPS complex subunit LAGE3Probable tRNA N6-adenosine threonylcarbamoyltransferase experimental SAS data

Sample:	EKC/KEOPS complex subunit GON7 monomer, 12 kDa Homo sapiens protein EKC/KEOPS complex subunit LAGE3 monomer, 15 kDa Homo sapiens protein Probable tRNA N6-adenosine threonylcarbamoyltransferase monomer, 36 kDa Homo sapiens protein
Buffer:	20 mM MES, 200 mM NaCl, 5 mM β-mercaptoethanol, pH: 6.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2017 May 12

Defects in t⁶A tRNA modification due to GON7 and YRDC mutations lead to Galloway-Mowat syndrome. Nat Commun 10(1):3967 (2019)
Arrondel C, Missoury S, Snoek R, Patat J, Menara G, Collinet B, Liger D, Durand D, Gribouval O, Boyer O, Buscara L, Martin G, Machuca E, Nevo F, Lescop E, Braun DA, Boschat AC, Sanquer S, Guerrera IC, Revy P, Parisot M, Masson C, Boddaert N, Charbit M, Decramer S, Novo R, Macher MA, Ranchin B, Bacchetta J, Laurent A, Collardeau-Frachon S, van Eerde AM, Hildebrandt F, Magen D, Antignac C, van Tilbeurgh H, Mollet G

R_g^Guinier	3.1	nm
D_max	11.5	nm
Volume^Porod	91	nm³

SASDG44 – Adenovirus Ad3 fibre knob in complex with the EC2-EC3 fragment of desmoglein 2

Human adenovirus serotype 3 fibre knobHuman desmoglein 2 extracellular domain 2 and 3 experimental SAS data

Sample:	Human adenovirus serotype 3 fibre knob trimer, 69 kDa Human adenovirus serotype … protein Human desmoglein 2 extracellular domain 2 and 3 dimer, 55 kDa Homo sapiens protein
Buffer:	20 mM Tris-HCl, 150 mM NaCl, 3 mM CaCl2, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2017 Jan 26

Intermediate-resolution crystal structure of the human adenovirus B serotype 3 fibre knob in complex with the EC2-EC3 fragment of desmoglein 2. Acta Crystallogr F Struct Biol Commun 75(Pt 12):750-757 (2019)
Vassal-Stermann E, Hutin S, Fender P, Burmeister WP

R_g^Guinier	3.4	nm
D_max	11.2	nm
Volume^Porod	188	nm³

SASDKS3 – The dimeric ectodomain of the alkali-sensing insulin receptor–related receptor (ectoIRR) at pH7

Insulin receptor-related protein experimental SAS data

Sample:	Insulin receptor-related protein dimer, 201 kDa Homo sapiens protein
Buffer:	150 mM NaCl, 20 mM Tris, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Oct 22

The dimeric ectodomain of the alkali-sensing insulin receptor-related receptor (ectoIRR) has a droplike shape. J Biol Chem 294(47):17790-17798 (2019)
Shtykova EV, Petoukhov MV, Mozhaev AA, Deyev IE, Dadinova LA, Loshkarev NA, Goryashchenko AS, Bocharov EV, Jeffries CM, Svergun DI, Batishchev OV, Petrenko AG

R_g^Guinier	5.4	nm
D_max	19.5	nm
Volume^Porod	444	nm³

SASDKT3 – The dimeric ectodomain of the alkali-sensing insulin receptor–related receptor (ectoIRR) at pH9

Sample:	Insulin receptor-related protein dimer, 201 kDa Homo sapiens protein
Buffer:	150 mM NaCl, 20 mM Tris, pH: 9
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Oct 22

R_g^Guinier	5.3	nm
D_max	19.0	nm
Volume^Porod	430	nm³

SASDFZ3 – Escherichia coli YjhC

Escherichia coli YjhC experimental SAS data

Sample:	Escherichia coli YjhC dimer, 86 kDa Escherichia coli protein
Buffer:	20 mM Tris, 150 mM NaCl, 0.1 % (w/v) sodium azide, 5 % (v/v) glycerol, pH: 8
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2018 Apr 12

On the structure and function of Escherichia coli YjhC: an oxidoreductase involved in bacterial sialic acid metabolism. Proteins (2019)
Horne CR, Kind L, Davies JS, Dobson RCJ

R_g^Guinier	3.1	nm
D_max	10.7	nm
Volume^Porod	130	nm³

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