Small Angle Scattering Biological Data Bank SASBDB

Browse by MODEL: Hybrid

SASDCM5 – Vaccinia virus binary E9 polymerase / DNA complex

DNA polymerase E9 exo- mutantDNA oligomer template-primer hairpin experimental SAS data

Sample:	DNA polymerase E9 exo- mutant monomer, 117 kDa Vaccinia virus protein DNA oligomer template-primer hairpin monomer, 9 kDa synthetic construct DNA
Buffer:	20 mM Tris HCl, 100 mM NaCl, 4 mM DTT, pH: 7
Experiment:	SAXS data collected at BM29, ESRF on 2015 Mar 12

The vaccinia virus DNA polymerase structure provides insights into the mode of processivity factor binding. Nat Commun 8(1):1455 (2017)
Tarbouriech N, Ducournau C, Hutin S, Mas PJ, Man P, Forest E, Hart DJ, Peyrefitte CN, Burmeister WP, Iseni F

R_g^Guinier	3.5	nm
D_max	12.0	nm
Volume^Porod	210	nm³

SASDCN5 – Vaccinia virus DNA polymerase

Sample:	DNA polymerase E9 monomer, 117 kDa Vaccinia virus protein
Buffer:	20 mM Tris HCl, 100 mM NaCl, 4 mM DTT, pH: 7
Experiment:	SAXS data collected at BM29, ESRF on 2014 Nov 27

R_g^Guinier	3.9	nm
D_max	12.0	nm
Volume^Porod	202	nm³

SASDBS9 – TET12SN

Sample:	TET12(1.10)SN-f5 monomer, 53 kDa synthetic construct protein
Buffer:	20 mM Tris 150 mM NaCl 10% glycerol, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2015 Oct 28

Design of coiled-coil protein-origami cages that self-assemble in vitro and in vivo. Nat Biotechnol 35(11):1094-1101 (2017)
Ljubetič A, Lapenta F, Gradišar H, Drobnak I, Aupič J, Strmšek Ž, Lainšček D, Hafner-Bratkovič I, Majerle A, Krivec N, Benčina M, Pisanski T, Veličković TĆ, Round A, Carazo JM, Melero R, Jerala R

R_g^Guinier	3.5	nm
D_max	12.0	nm
Volume^Porod	107	nm³

SASDCR7 – TET12(1.10)SN-c6

Sample:	TET12(1.10)SN-c6 monomer, 54 kDa synthetic construct protein
Buffer:	20 mM Tris 150 mM NaCl 10% glycerol, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Jun 10

R_g^Guinier	3.6	nm
D_max	11.5	nm
Volume^Porod	145	nm³

SASDCS7 – TET12(1.10)SN-f9

Sample:	TET12(1.10)SN-f9 monomer, 57 kDa synthetic construct protein
Buffer:	20 mM Tris 150 mM NaCl 10% glycerol, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Jun 10

R_g^Guinier	3.9	nm
D_max	12.9	nm
Volume^Porod	153	nm³

SASDCT7 – PYR16(4.6)SN-f5

Sample:	PYR16(4.6)SN-f5 monomer, 72 kDa synthetic construct protein
Buffer:	20 mM Tris 150 mM NaCl 10% glycerol, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2016 Feb 16

R_g^Guinier	3.8	nm
D_max	11.3	nm
Volume^Porod	136	nm³

SASDCU7 – TRIP18(7.5R)SN-f5

Sample:	TRIP18(7.5R)SN-f5 monomer, 82 kDa synthetic construct protein
Buffer:	20 mM Tris 150 mM NaCl 10% glycerol, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Jun 10

R_g^Guinier	4.2	nm
D_max	14.5	nm
Volume^Porod	210	nm³

SASDCV7 – TET12(2.3)SN-f5b

Sample:	TET12(2.3)SN-f5b monomer, 57 kDa synthetic construct protein
Buffer:	20 mM Tris 150 mM NaCl 10% glycerol, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Jun 10

R_g^Guinier	3.5	nm
D_max	11.5	nm
Volume^Porod	105	nm³

SASDCW7 – TET12(1.10)S-f5

Sample:	TET12(1.10)S-f5 monomer, 53 kDa synthetic construct protein
Buffer:	20 mM Tris 150 mM NaCl 10% glycerol, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2016 Apr 7

R_g^Guinier	3.6	nm
D_max	13.5	nm
Volume^Porod	210	nm³

SASDCX7 – TET12(1.10)S-f5b

Sample:	TET12(1.10)S-f5b monomer, 53 kDa synthetic construct protein
Buffer:	20 mM Tris 150 mM NaCl 10% glycerol, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2016 Apr 7

R_g^Guinier	3.4	nm
D_max	11.2	nm
Volume^Porod	127	nm³

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