Small Angle Scattering Biological Data Bank SASBDB

Browse by MODEL: Hybrid

SASDCY7 – TET12(1.10)S-c6

Sample:	TET12(1.10)S-c6 monomer, 55 kDa synthetic construct protein
Buffer:	20 mM Tris 150 mM NaCl 10% glycerol, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2016 Apr 7

Design of coiled-coil protein-origami cages that self-assemble in vitro and in vivo. Nat Biotechnol 35(11):1094-1101 (2017)
Ljubetič A, Lapenta F, Gradišar H, Drobnak I, Aupič J, Strmšek Ž, Lainšček D, Hafner-Bratkovič I, Majerle A, Krivec N, Benčina M, Pisanski T, Veličković TĆ, Round A, Carazo JM, Melero R, Jerala R

R_g^Guinier	3.8	nm
D_max	13.0	nm
Volume^Porod	107	nm³

SASDCZ7 – TET12(1.6)S-c6b

Sample:	TET12(1.6)S-c6b monomer, 54 kDa synthetic construct protein
Buffer:	20 mM Tris 150 mM NaCl 10% glycerol, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Apr 7

R_g^Guinier	3.5	nm
D_max	11.5	nm
Volume^Porod	114	nm³

SASDCB2 – Rab family protein CtRoc-COR nucleotide free

Rab family protein experimental SAS data

Sample:	Rab family protein dimer, 130 kDa Chlorobaculum tepidum protein
Buffer:	20 mM HEPES 150 mM NaCl 5 mM MgCl2 5% Glycerol 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2017 Feb 1

A homologue of the Parkinson's disease-associated protein LRRK2 undergoes a monomer-dimer transition during GTP turnover. Nat Commun 8(1):1008 (2017)
Deyaert E, Wauters L, Guaitoli G, Konijnenberg A, Leemans M, Terheyden S, Petrovic A, Gallardo R, Nederveen-Schippers LM, Athanasopoulos PS, Pots H, Van Haastert PJM, Sobott F, Gloeckner CJ, Efremov R, Kortholt A, Versées W

R_g^Guinier	3.4	nm
D_max	10.0	nm
Volume^Porod	211	nm³

SASDCZ2 – Yeast CTDsome: transcription termination factor Rtt103p/C-terminal domain of DNA-directed RNA polymerase II subunit RPB1 (Rtt103 1-246-phospho-mimetic complex)

Regulator of Ty1 transposition protein 103DNA-directed RNA polymerase II subunit RPB1 experimental SAS data

Sample:	Regulator of Ty1 transposition protein 103 dimer, 57 kDa Saccharomyces cerevisiae protein DNA-directed RNA polymerase II subunit RPB1 monomer, 13 kDa Saccharomyces cerevisiae protein
Buffer:	25 mM KH2PO4, 300 mM KCl, 10 mM BME, pH: 6.5
Experiment:	SAXS data collected at Rigaku BioSAXS-1000, CEITEC on 2016 Apr 12

Structure and dynamics of the RNAPII CTDsome with Rtt103. Proc Natl Acad Sci U S A 114(42):11133-11138 (2017)
Jasnovidova O, Klumpler T, Kubicek K, Kalynych S, Plevka P, Stefl R

R_g^Guinier	5.3	nm
D_max	21.0	nm
Volume^Porod	561	nm³

SASDCK2 – Glucose Isomerase - Streptomyces rubiginosus

Sample:	Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer:	25 mM MOPS, 250 mM NaCl, 50 mM KCl, 2 mM TCEP, 0.1% NaN3, pH: 7.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Mar 9

2017 publication guidelines for structural modelling of small-angle scattering data from biomolecules in solution: an update. Acta Crystallogr D Struct Biol 73(Pt 9):710-728 (2017)
Trewhella J, Duff AP, Durand D, Gabel F, Guss JM, Hendrickson WA, Hura GL, Jacques DA, Kirby NM, Kwan AH, Pérez J, Pollack L, Ryan TM, Sali A, Schneidman-Duhovny D, Schwede T, Svergun DI, Sugiyama M, Tainer JA, Vachette P, Westbrook J, Whitten AE

R_g^Guinier	3.3	nm
D_max	9.2	nm
Volume^Porod	229	nm³

SASDCJ3 – Bovine Serum Albumin

Sample:	Serum albumin monomer, 66 kDa Bos taurus protein
Buffer:	25 mM MOPS, 250 mM NaCl, 50 mM KCl, 2 mM TCEP, 0.1% NaN3, pH: 7.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Mar 9

R_g^Guinier	2.8	nm
D_max	8.7	nm
Volume^Porod	101	nm³

SASDB48 – Major viral transcription factor ICP4/Immediate Early 3 DNA (ICP4N IE3_19mer) complex

Major viral transcription factor ICP4Immediate Early 3 experimental SAS data

Sample:	Major viral transcription factor ICP4 dimer, 49 kDa Human alphaherpesvirus 1 protein Immediate Early 3 dimer, 12 kDa DNA
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at B21, Diamond Light Source on 2015 Jul 27

The herpes viral transcription factor ICP4 forms a novel DNA recognition complex. Nucleic Acids Res 45(13):8064-8078 (2017)
Tunnicliffe RB, Lockhart-Cairns MP, Levy C, Mould AP, Jowitt TA, Sito H, Baldock C, Sandri-Goldin RM, Golovanov AP

R_g^Guinier	2.5	nm
D_max	8.3	nm
Volume^Porod	90	nm³

SASDBD9 – ABC transporter periplasmic substrate-binding protein TupA measured in the absence of metal ligand (tungstate or molybdate)

ABC transporter periplasmic substrate-binding protein experimental SAS data

Sample:	ABC transporter periplasmic substrate-binding protein monomer, 30 kDa Desulfovibrio alaskensis protein
Buffer:	5 mM Tris-HCl, pH: 7.6
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Dec 7

Highly selective tungstate transporter protein TupA from Desulfovibrio alaskensis G20. Sci Rep 7(1):5798 (2017)
Otrelo-Cardoso AR, Nair RR, Correia MAS, Cordeiro RSC, Panjkovich A, Svergun DI, Santos-Silva T, Rivas MG

R_g^Guinier	2.4	nm
D_max	9.6	nm
Volume^Porod	51	nm³

SASDBE9 – ABC transporter periplasmic substrate-binding protein TupA measured in the absence of metal ligand (tungstate or molybdate)

Sample:	ABC transporter periplasmic substrate-binding protein monomer, 30 kDa Desulfovibrio alaskensis protein
Buffer:	5 mM Tris-HCl, pH: 7.6
Experiment:	SAXS data collected at BM29, ESRF on 2014 Jun 21

R_g^Guinier	2.4	nm
D_max	9.5	nm
Volume^Porod	49	nm³

SASDBF9 – ABC transporter periplasmic substrate-binding protein TupA measured in the presence of tungstate

Sample:	ABC transporter periplasmic substrate-binding protein monomer, 30 kDa Desulfovibrio alaskensis protein
Buffer:	5 mM Tris-HCl, pH: 7.6
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Dec 7

R_g^Guinier	2.3	nm
D_max	9.0	nm
Volume^Porod	46	nm³

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