Browse by MODEL: Hybrid

SASDNG9 – GIFT based desmeared and regularized SAXS profile on ADP-ribosylation factor-like protein 15 (apo conditions)

ADP-ribosylation factor-like protein 15 experimental SAS data
GASBOR model
Sample: ADP-ribosylation factor-like protein 15 monomer, 22 kDa Homo sapiens protein
Buffer: 20 mM Tris, 100 mM NaCl, 1 mM PMSF, 10% glycerol, 1 mM DTT, pH: 8
Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) on 2018 Jul 10
ARL15, a GTPase implicated in rheumatoid arthritis, potentially repositions its truncated N-terminus as a function of guanine nucleotide binding International Journal of Biological Macromolecules 254:127898 (2024)
Saini M, Upadhyay N, Dhiman K, Manjhi S, Kattuparambil A, Ghoshal A, Arya R, Dey S, Sharma A, Aduri R, Thelma B, Ashish F, Kundu S
RgGuinier 1.8 nm
Dmax 6.1 nm
VolumePorod 33 nm3

SASDNH9 – GIFT based desmeared and regularized SAXS profile on ADP-ribosylation factor-like protein 15 with GDP

ADP-ribosylation factor-like protein 15 experimental SAS data
GASBOR model
Sample: ADP-ribosylation factor-like protein 15 monomer, 22 kDa Homo sapiens protein
Buffer: 20 mM Tris, 100 mM NaCl, 1 mM PMSF, 10% glycerol, 1 mM DTT, pH: 8
Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) on 2018 Jul 10
ARL15, a GTPase implicated in rheumatoid arthritis, potentially repositions its truncated N-terminus as a function of guanine nucleotide binding International Journal of Biological Macromolecules 254:127898 (2024)
Saini M, Upadhyay N, Dhiman K, Manjhi S, Kattuparambil A, Ghoshal A, Arya R, Dey S, Sharma A, Aduri R, Thelma B, Ashish F, Kundu S
RgGuinier 2.0 nm
Dmax 7.6 nm
VolumePorod 38 nm3

SASDNJ9 – GIFT based desmeared and regularized SAXS profile on ADP-ribosylation factor-like protein 15 with GTP

ADP-ribosylation factor-like protein 15 experimental SAS data
GASBOR model
Sample: ADP-ribosylation factor-like protein 15 monomer, 22 kDa Homo sapiens protein
Buffer: 20 mM Tris, 100 mM NaCl, 1 mM PMSF, 10% glycerol, 1 mM DTT, pH: 8
Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) on 2018 Jul 10
ARL15, a GTPase implicated in rheumatoid arthritis, potentially repositions its truncated N-terminus as a function of guanine nucleotide binding International Journal of Biological Macromolecules 254:127898 (2024)
Saini M, Upadhyay N, Dhiman K, Manjhi S, Kattuparambil A, Ghoshal A, Arya R, Dey S, Sharma A, Aduri R, Thelma B, Ashish F, Kundu S
RgGuinier 2.0 nm
Dmax 7.7 nm
VolumePorod 38 nm3

SASDTU4 – 24×FerSUMO - Chimeric protein complex of ferritin from Helicobacter pylori fused to an N-terminal His6-SUMO protein tag

Bacterial non-heme ferritin (N19Q, I59V, N-terminal His-SUMO fusion) experimental SAS data
CORAL model
Sample: Bacterial non-heme ferritin (N19Q, I59V, N-terminal His-SUMO fusion) 24-mer, 755 kDa Helicobacter pylori (strain … protein
Buffer: 25 mM Tris, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at Rigaku MicroMax 007-HF, Moscow Institute of Physics and Technology (MIPT) on 2022 Jul 30
Ferritin-based fusion protein shows octameric deadlock state of self-assembly Biochemical and Biophysical Research Communications 690:149276 (2024)
Sudarev V, Gette M, Bazhenov S, Tilinova O, Zinovev E, Manukhov I, Kuklin A, Ryzhykau Y, Vlasov A
RgGuinier 7.6 nm
Dmax 25.0 nm
VolumePorod 2010 nm3

SASDTV4 – 8×FerSUMO - Chimeric protein complex of ferritin from Helicobacter pylori fused to an N-terminal His6-SUMO protein tag

Bacterial non-heme ferritin (N19Q, I59V, N-terminal His-SUMO fusion) experimental SAS data
PHYRE2 model
Sample: Bacterial non-heme ferritin (N19Q, I59V, N-terminal His-SUMO fusion) octamer, 252 kDa Helicobacter pylori (strain … protein
Buffer: 25 mM Tris, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at Rigaku MicroMax 007-HF, Moscow Institute of Physics and Technology (MIPT) on 2022 Jul 17
Ferritin-based fusion protein shows octameric deadlock state of self-assembly Biochemical and Biophysical Research Communications 690:149276 (2024)
Sudarev V, Gette M, Bazhenov S, Tilinova O, Zinovev E, Manukhov I, Kuklin A, Ryzhykau Y, Vlasov A
RgGuinier 5.4 nm
Dmax 16.0 nm
VolumePorod 618 nm3

SASDTW4 – Ferritin from Helicobacter pylori with an N-terminal His6-tag

Bacterial non-heme ferritin (N19Q, I59V) experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Bacterial non-heme ferritin (N19Q, I59V) 24-mer, 480 kDa Helicobacter pylori (strain … protein
Buffer: 20 mM Tris, pH: 8
Experiment: SAXS data collected at Rigaku MicroMax 007-HF, Moscow Institute of Physics and Technology (MIPT) on 2022 Dec 14
Ferritin-based fusion protein shows octameric deadlock state of self-assembly Biochemical and Biophysical Research Communications 690:149276 (2024)
Sudarev V, Gette M, Bazhenov S, Tilinova O, Zinovev E, Manukhov I, Kuklin A, Ryzhykau Y, Vlasov A
RgGuinier 5.6 nm
Dmax 14.5 nm
VolumePorod 674 nm3

SASDTK5 – Heme acquisition system protein A (39.9 kDa dimer from Pseudomonas protegens PF-5)

Heme acquisition protein HasAp experimental SAS data
DAMMIN model
Sample: Heme acquisition protein HasAp dimer, 38 kDa Pseudomonas fluorescens (strain … protein
Buffer: 50 mM CHES, 5 % glycerol, pH: 9.5
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2023 Feb 24
Heme-substituted protein assembly bridged by synthetic porphyrin: achieving controlled configuration while maintaining rotational freedom RSC Advances 14(13):8829-8836 (2024)
Inaba H, Shisaka Y, Ariyasu S, Sakakibara E, Ueda G, Aiba Y, Shimizu N, Sugimoto H, Shoji O
RgGuinier 2.8 nm
Dmax 9.1 nm
VolumePorod 54 nm3

SASDUN2 – DiTET-cc (coiled coil protein origami tetrahedron assembled from two cyclized chains)

cyRH1 (cyclic bitrigon from 6 coiled coil segments)cyRH2 (cyclic bitrigon from 6 coiled coil segments) experimental SAS data
SREFLEX model
Sample: CyRH1 (cyclic bitrigon from 6 coiled coil segments) monomer, 26 kDa synthetic construct protein
CyRH2 (cyclic bitrigon from 6 coiled coil segments) monomer, 26 kDa synthetic construct protein
Buffer: 20 mM Tris, 150 mM NaCL, 3% glycerol, 1mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 30
Preorganized cyclic modules facilitate the self-assembly of protein nanostructures Chemical Science (2024)
Snoj J, Lapenta F, Jerala R
RgGuinier 3.1 nm
Dmax 8.8 nm
VolumePorod 150 nm3

SASDUP2 – DiTET-nc (coiled coil protein origami tetrahedron assembled from one linear and one cyclized chain)

RH1 (Linear coiled-coil protein origami structure consisting of 6 coiled-coil forming segments)cyRH2 (cyclic bitrigon from 6 coiled coil segments) experimental SAS data
SREFLEX model
Sample: RH1 (Linear coiled-coil protein origami structure consisting of 6 coiled-coil forming segments) monomer, 26 kDa synthetic construct protein
CyRH2 (cyclic bitrigon from 6 coiled coil segments) monomer, 26 kDa synthetic construct protein
Buffer: 50 mM Tris, 150 mM NaCL, 3% glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Aug 29
Preorganized cyclic modules facilitate the self-assembly of protein nanostructures Chemical Science (2024)
Snoj J, Lapenta F, Jerala R
RgGuinier 3.6 nm
Dmax 12.5 nm
VolumePorod 161 nm3

SASDUQ2 – DiTET-nn (coiled coil protein origami assembled from two linear chains)

RH1 (Linear coiled-coil protein origami structure consisting of 6 coiled-coil forming segments)RH2 (Linear coiled-coil protein origami structure consisting of 6 coiled-coil forming segments) experimental SAS data
SREFLEX model
Sample: RH1 (Linear coiled-coil protein origami structure consisting of 6 coiled-coil forming segments) monomer, 26 kDa synthetic construct protein
RH2 (Linear coiled-coil protein origami structure consisting of 6 coiled-coil forming segments) monomer, 26 kDa synthetic construct protein
Buffer: 20 mM Tris, 150 mM NaCL, 3% glycerol, 1mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Aug 9
Preorganized cyclic modules facilitate the self-assembly of protein nanostructures Chemical Science (2024)
Snoj J, Lapenta F, Jerala R
RgGuinier 4.3 nm
Dmax 14.7 nm
VolumePorod 161 nm3