Small Angle Scattering Biological Data Bank SASBDB

Browse by MODEL: Hybrid

SASDRZ8 – Single-chain Archaeoglobus fulgidus argonaute fAfAgo-fusion variant complexed with an 11 base pair DNA oligoduplex in low salt buffer

single-chain full Archaeoglobus fulgidus Argonaute5'-end phosphorylated DNA guide strand, 11 nt (MZ864)DNA target strand, 11 nt (MZ865) experimental SAS data

Sample:	Single-chain full Archaeoglobus fulgidus Argonaute monomer, 78 kDa Archaeoglobus fulgidus protein 5'-end phosphorylated DNA guide strand, 11 nt (MZ864) monomer, 3 kDa DNA DNA target strand, 11 nt (MZ865) monomer, 3 kDa DNA
Buffer:	20 mM TrisHCl pH7.5, 200 mM NaCl, 5 mM MgCl2, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Aug 31

The missing part: the Archaeoglobus fulgidus Argonaute forms a functional heterodimer with an N-L1-L2 domain protein. Nucleic Acids Res (2024)
Manakova E, Golovinas E, Pocevičiūtė R, Sasnauskas G, Silanskas A, Rutkauskas D, Jankunec M, Zagorskaitė E, Jurgelaitis E, Grybauskas A, Venclovas Č, Zaremba M

R_g^Guinier	2.9	nm
D_max	9.6	nm
Volume^Porod	125	nm³

SASDPK3 – Vibrio cholerae ParD2:ParE2 complex bound to 21-bp DNA operator box

Antitoxin ParDToxin21-bp DNA operator fragment experimental SAS data

Sample:	Antitoxin ParD hexamer, 54 kDa Vibrio cholerae serotype … protein Toxin, 25 kDa Vibrio cholerae serotype … protein 21-bp DNA operator fragment monomer, 13 kDa Vibrio cholerae O1 DNA
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2020 Jul 18

Toxin:antitoxin ratio sensing autoregulation of the Vibrio cholerae parDE2 module. Sci Adv 10(1):eadj2403 (2024)
Garcia-Rodriguez G, Girardin Y, Kumar Singh R, Volkov AN, Van Dyck J, Muruganandam G, Sobott F, Charlier D, Loris R

R_g^Guinier	3.2	nm
D_max	10.0	nm
Volume^Porod	140	nm³

SASDPL3 – Vibrio cholerae ParD2:ParE2 complex bound to 31-bp DNA operator box

Antitoxin ParDToxin31-bp DNA operator box experimental SAS data

Sample:	Antitoxin ParD hexamer, 54 kDa Vibrio cholerae serotype … protein Toxin, 25 kDa Vibrio cholerae serotype … protein 31-bp DNA operator box monomer, 19 kDa Vibrio cholerae O1 DNA
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2019 Dec 4

R_g^Guinier	3.3	nm
D_max	10.5	nm
Volume^Porod	160	nm³

SASDPM3 – Vibrio cholerae ParD2:ParE2 complex bound to 33-bp DNA operator fragment

Antitoxin ParDToxin33-bp DNA operator fragment experimental SAS data

Sample:	Antitoxin ParD hexamer, 54 kDa Vibrio cholerae serotype … protein Toxin, 25 kDa Vibrio cholerae serotype … protein 33-bp DNA operator fragment monomer, 20 kDa Vibrio cholerae O1 DNA
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2020 Jul 18

R_g^Guinier	3.2	nm
D_max	10.0	nm
Volume^Porod	150	nm³

SASDPN3 – Vibrio cholerae ParD2:ParE2 antitoxin:toxin complex

Antitoxin ParDToxin experimental SAS data

Sample:	Antitoxin ParD hexamer, 54 kDa Vibrio cholerae serotype … protein Toxin, 25 kDa Vibrio cholerae serotype … protein
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2017 Mar 6

R_g^Guinier	3.0	nm
D_max	11.0	nm
Volume^Porod	140	nm³

SASDPE2 – Starch adherence system protein 6 (Sas6)

Dockerin type I repeat experimental SAS data

Sample:	Dockerin type I repeat monomer, 69 kDa Ruminococcus bromii protein
Buffer:	phosphate buffered saline, 1 mM TCEP, pH: 7
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 14

The Ruminococcus bromii amylosome protein Sas6 binds single and double helical α-glucan structures in starch. Nat Struct Mol Biol (2024)
Photenhauer AL, Villafuerte-Vega RC, Cerqueira FM, Armbruster KM, Mareček F, Chen T, Wawrzak Z, Hopkins JB, Vander Kooi CW, Janeček Š, Ruotolo BT, Koropatkin NM

R_g^Guinier	3.0	nm
D_max	9.0	nm
Volume^Porod	97	nm³

SASDLX2 – Immunoglobulin G4 (IgG4) Deglycosylated

Immunoglobulin G subclass 4 experimental SAS data

Sample:	Immunoglobulin G subclass 4 monomer, 145 kDa Homo sapiens protein
Buffer:	20 mM L-histidine, 138 mM NaCl, and 2.6 mM KCl buffer, pH: 6
Experiment:	SAXS data collected at B21, Diamond Light Source on 2017 Oct 20

Using atomistic solution scattering modelling to elucidate the role of the Fc glycans in human IgG4. PLoS One 19(4):e0300964 (2024)
Spiteri VA, Doutch J, Rambo RP, Bhatt JS, Gor J, Dalby PA, Perkins SJ

R_g^Guinier	4.9	nm
D_max	16.9	nm
Volume^Porod	258	nm³

SASDLY2 – Immunoglobulin G4 (IgG4) Glycosylated

Sample:	Immunoglobulin G subclass 4 monomer, 145 kDa Homo sapiens protein
Buffer:	20 mM L-histidine, 138 mM NaCl, and 2.6 mM KCl buffer, pH: 6
Experiment:	SAXS data collected at B21, Diamond Light Source on 2017 Oct 20

R_g^Guinier	4.9	nm
D_max	16.3	nm
Volume^Porod	256	nm³

SASDNK8 – The C-terminal region of histone-lysine N-methyltransferase NSD3: SET-PHD4 construct

Histone-lysine N-methyltransferase NSD3 experimental SAS data

Sample:	Histone-lysine N-methyltransferase NSD3 monomer, 40 kDa Homo sapiens protein
Buffer:	0.5 M NaCl, 20 mM Tris-HCl, 5 mM DTT, pH: 8.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Nov 25

Structural insights into the C-terminus of the histone-lysine N-methyltransferase NSD3 by small-angle X-ray scattering. Front Mol Biosci 11:1191246 (2024)
Belviso BD, Shen Y, Carrozzini B, Morishita M, di Luccio E, Caliandro R

R_g^Guinier	3.4	nm
D_max	13.2	nm
Volume^Porod	75	nm³

SASDNL8 – The C-terminal region of histone-lysine N-methyltransferase NSD3: PWWP2-SET construct

Sample:	Histone-lysine N-methyltransferase NSD3 monomer, 43 kDa Homo sapiens protein
Buffer:	0.5 M NaCl, 20 mM Tris-HCl, 5 mM DTT, pH: 8.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Nov 25

R_g^Guinier	3.1	nm
D_max	11.2	nm
Volume^Porod	64	nm³

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