Browse by MODEL: Hybrid

SASDQ55 – DNA-binding protein from starved cells: DgrDps-D43A mutant + 48 Zn2+ in 50 mM MOPS pH 7.0, 230 mM NaCl

DNA protection during starvation, DPS-D43A (Ferritin superfamily) dodecamer D43A mutation experimental SAS data
GASBOR model
Sample: DNA protection during starvation, DPS-D43A (Ferritin superfamily) dodecamer D43A mutation dodecamer, 270 kDa Deinococcus grandis protein
Buffer: 50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8
Controlled modulation of the dynamics of the Deinococcus grandis Dps N-terminal tails by divalent metals. Protein Sci :e4567 (2023)
Guerra JPL, Blanchet CE, Vieira BJC, Waerenborgh JC, Jones NC, Hoffmann SV, Pereira AS, Tavares P
RgGuinier 4.4 nm
Dmax 20.7 nm
VolumePorod 409 nm3

SASDQ65 – DNA-binding protein from starved cells: DgrDps-D43A mutant + 24 Co2+ in 50 mM MOPS pH 7.0, 230 mM NaCl

DNA protection during starvation, DPS-D43A (Ferritin superfamily) dodecamer D43A mutation experimental SAS data
GASBOR model
Sample: DNA protection during starvation, DPS-D43A (Ferritin superfamily) dodecamer D43A mutation dodecamer, 270 kDa Deinococcus grandis protein
Buffer: 50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8
Controlled modulation of the dynamics of the Deinococcus grandis Dps N-terminal tails by divalent metals. Protein Sci :e4567 (2023)
Guerra JPL, Blanchet CE, Vieira BJC, Waerenborgh JC, Jones NC, Hoffmann SV, Pereira AS, Tavares P
RgGuinier 4.5 nm
Dmax 20.6 nm
VolumePorod 404 nm3

SASDQ75 – DNA-binding protein from starved cells: DgrDps-D43A mutant + 24 Mn2+ in 50 mM MOPS pH 7.0, 230 mM NaCl

DNA protection during starvation, DPS-D43A (Ferritin superfamily) dodecamer D43A mutation experimental SAS data
GASBOR model
Sample: DNA protection during starvation, DPS-D43A (Ferritin superfamily) dodecamer D43A mutation dodecamer, 270 kDa Deinococcus grandis protein
Buffer: 50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8
Controlled modulation of the dynamics of the Deinococcus grandis Dps N-terminal tails by divalent metals. Protein Sci :e4567 (2023)
Guerra JPL, Blanchet CE, Vieira BJC, Waerenborgh JC, Jones NC, Hoffmann SV, Pereira AS, Tavares P
RgGuinier 4.5 nm
Dmax 19.5 nm
VolumePorod 431 nm3

SASDQ37 – N-terminal GST-like domain of Plasmodium berghei cytosolic glutamyl-tRNA synthetase

Glutamate--tRNA ligase experimental SAS data
OLIGOMER model
Sample: Glutamate--tRNA ligase dimer, 57 kDa Plasmodium berghei (strain … protein
Buffer: 25 mM HEPES-NaOH, 300 mM NaCl, 5% (v/v) glycerol, 0.005% (m/v) n-dodecyl beta-D-maltoside, 5 mM 2-mercaptoethanol, pH: 7
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Oct 9
Solution x-ray scattering highlights discrepancies in Plasmodium multi-aminoacyl-tRNA synthetase complexes. Protein Sci :e4564 (2023)
Jaramillo Ponce JR, Théobald-Dietrich A, Bénas P, Paulus C, Sauter C, Frugier M
RgGuinier 3.0 nm
Dmax 11.5 nm
VolumePorod 96 nm3

SASDQ47 – N-terminal GST-like domain of Plasmodium berghei tRNA import protein

tRNA import protein tRIP experimental SAS data
CORAL model
Sample: TRNA import protein tRIP dimer, 49 kDa Plasmodium berghei (strain … protein
Buffer: 25 mM HEPES-NaOH, 300 mM NaCl, 5% (v/v) glycerol, 0.005% (m/v) n-dodecyl beta-D-maltoside, 5 mM 2-mercaptoethanol, pH: 7
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Oct 9
Solution x-ray scattering highlights discrepancies in Plasmodium multi-aminoacyl-tRNA synthetase complexes. Protein Sci :e4564 (2023)
Jaramillo Ponce JR, Théobald-Dietrich A, Bénas P, Paulus C, Sauter C, Frugier M
RgGuinier 2.9 nm
Dmax 11.0 nm
VolumePorod 68 nm3

SASDQ57 – The scaffold of Plasmodium berghei multi aminoacyl-tRNA synthetase complexes

Chimeric construct containing the GST-like domains of P. berghei tRNA import protein and glutamyl-tRNA synthetase experimental SAS data
CORAL model
Sample: Chimeric construct containing the GST-like domains of P. berghei tRNA import protein and glutamyl-tRNA synthetase dimer, 105 kDa Plasmodium berghei ANKA protein
Buffer: 25 mM HEPES-NaOH, 300 mM NaCl, 5% (v/v) glycerol, 0.005% (m/v) n-dodecyl beta-D-maltoside, 5 mM 2-mercaptoethanol, pH: 7
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Oct 9
Solution x-ray scattering highlights discrepancies in Plasmodium multi-aminoacyl-tRNA synthetase complexes. Protein Sci :e4564 (2023)
Jaramillo Ponce JR, Théobald-Dietrich A, Bénas P, Paulus C, Sauter C, Frugier M
RgGuinier 3.9 nm
Dmax 14.1 nm
VolumePorod 145 nm3

SASDQ67 – Plasmodium berghei multi aminoacyl-tRNA synthetase complex, Q-complex containing the GST-like domains of tRNA import protein, glutamyl-tRNA synthetase and glutaminyl-tRNA synthetase

Glutamine--tRNA ligaseGlutamate--tRNA ligasetRNA import protein tRIP experimental SAS data
CORAL model
Sample: Glutamine--tRNA ligase dimer, 46 kDa Plasmodium berghei (strain … protein
Glutamate--tRNA ligase dimer, 55 kDa Plasmodium berghei (strain … protein
TRNA import protein tRIP dimer, 48 kDa Plasmodium berghei (strain … protein
Buffer: 25 mM HEPES-NaOH, 300 mM NaCl, 5% (v/v) glycerol, 0.005% (m/v) n-dodecyl beta-D-maltoside, 5 mM 2-mercaptoethanol, pH: 7
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Oct 9
Solution x-ray scattering highlights discrepancies in Plasmodium multi-aminoacyl-tRNA synthetase complexes. Protein Sci :e4564 (2023)
Jaramillo Ponce JR, Théobald-Dietrich A, Bénas P, Paulus C, Sauter C, Frugier M
RgGuinier 4.6 nm
Dmax 17.1 nm
VolumePorod 198 nm3

SASDQ77 – Plasmodium berghei multi aminoacyl-tRNA synthetase complex, M-complex containing the GST-like domains of tRNA import protein, glutamyl-tRNA synthetase and methionyl-tRNA synthetase

Methionine--tRNA ligasetRNA import protein tRIPGlutamate--tRNA ligase experimental SAS data
CORAL model
Sample: Methionine--tRNA ligase dimer, 55 kDa Plasmodium berghei (strain … protein
TRNA import protein tRIP dimer, 48 kDa Plasmodium berghei (strain … protein
Glutamate--tRNA ligase dimer, 55 kDa Plasmodium berghei (strain … protein
Buffer: 25 mM HEPES-NaOH, 300 mM NaCl, 5% (v/v) glycerol, 0.005% (m/v) n-dodecyl beta-D-maltoside, 5 mM 2-mercaptoethanol, pH: 7
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Oct 9
Solution x-ray scattering highlights discrepancies in Plasmodium multi-aminoacyl-tRNA synthetase complexes. Protein Sci :e4564 (2023)
Jaramillo Ponce JR, Théobald-Dietrich A, Bénas P, Paulus C, Sauter C, Frugier M
RgGuinier 4.7 nm
Dmax 18.3 nm
VolumePorod 217 nm3

SASDM45 – TrYbe® trispecific antibody

Tri-specific antibody experimental SAS data
OTHER model
Sample: Tri-specific antibody monomer, 102 kDa Homo sapiens protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Apr 26
TRYBE®: an Fc-free antibody format with three monovalent targeting arms engineered for long in vivo half-life. MAbs 15(1):2160229 (2023)
Davé E, Durrant O, Dhami N, Compson J, Broadbridge J, Archer S, Maroof A, Whale K, Menochet K, Bonnaillie P, Barry E, Wild G, Peerboom C, Bhatta P, Ellis M, Hinchliffe M, Humphreys DP, Heywood SP
RgGuinier 4.1 nm
Dmax 13.0 nm
VolumePorod 157 nm3

SASDPV2 – original TIP60 (Truncated Icosahedral Protein composed of 60-mer fusion proteins)

Fusion protein of LSm and MyoX-coil experimental SAS data
DAMMIN model
Sample: Fusion protein of LSm and MyoX-coil, 1066 kDa Artificial protein protein
Buffer: 25 mM HEPES, 100 mM NaCl, 1 mM EDTA, 5% glycerol, pH: 8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 May 23
Reversible Assembly of an Artificial Protein Nanocage Using Alkaline Earth Metal Ions. J Am Chem Soc (2022)
Ohara N, Kawakami N, Arai R, Adachi N, Moriya T, Kawasaki M, Miyamoto K
RgGuinier 9.1 nm
Dmax 22.7 nm