Small Angle Scattering Biological Data Bank SASBDB

Browse by MODEL: Hybrid

SASDKL4 – High temperature requirement of human mitochondrial serine protease HTRA2 (S306A mutant; 10 mg/ml)

Serine protease HTRA2, mitochondrial experimental SAS data

Sample:	Serine protease HTRA2, mitochondrial hexamer, 210 kDa Homo sapiens protein
Buffer:	20 mM HEPES-NaOH (pH 7.4), 120 mM NaCl, 1 mM EDTA, and 2% glycerol, pH: 7.4
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Jul 1

Oligomeric assembly regulating mitochondrial HtrA2 function as examined by methyl-TROSY NMR Proceedings of the National Academy of Sciences 118(11):e2025022118 (2021)
Toyama Y, Harkness R, Lee T, Maynes J, Kay L

R_g^Guinier	4.2	nm
D_max	16.0	nm
Volume^Porod	250	nm³

SASDKM4 – High temperature requirement of human mitochondrial serine protease HTRA2 (S306A mutant; 5 mg/ml)

Sample:	Serine protease HTRA2, mitochondrial hexamer, 210 kDa Homo sapiens protein
Buffer:	20 mM HEPES-NaOH (pH 7.4), 120 mM NaCl, 1 mM EDTA, and 2% glycerol, pH: 7.4
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Jul 1

R_g^Guinier	3.8	nm
D_max	15.0	nm
Volume^Porod	213	nm³

SASDKN4 – High temperature requirement of human mitochondrial serine protease HTRA2 (S306A mutant; 2 mg/ml)

Sample:	Serine protease HTRA2, mitochondrial hexamer, 210 kDa Homo sapiens protein
Buffer:	20 mM HEPES-NaOH (pH 7.4), 120 mM NaCl, 1 mM EDTA, and 2% glycerol, pH: 7.4
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Jul 1

R_g^Guinier	3.6	nm
D_max	12.8	nm
Volume^Porod	203	nm³

SASDKP4 – High temperature requirement of human mitochondrial serine protease HTRA2 (S306A mutant; 1 mg/ml)

Sample:	Serine protease HTRA2, mitochondrial hexamer, 210 kDa Homo sapiens protein
Buffer:	20 mM HEPES-NaOH (pH 7.4), 120 mM NaCl, 1 mM EDTA, and 2% glycerol, pH: 7.4
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Jul 1

R_g^Guinier	3.4	nm
D_max	12.0	nm
Volume^Porod	168	nm³

SASDKY4 – DNA binding protein of starvation (no EDTA)

DNA protection during starvation protein experimental SAS data

Sample:	DNA protection during starvation protein dodecamer, 225 kDa Escherichia coli protein
Buffer:	20мМ Tris-HCl, 100 мМ NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Nov 19

Spatial Organization of Dps and DNA-Dps Complexes. J Mol Biol :166930 (2021)
Dubrovin EV, Dadinova LA, Petoukhov MV, Yu Soshinskaya E, Mozhaev AA, Klinov DV, Schäffer TE, Shtykova EV, Batishchev OV

R_g^Guinier	3.8	nm
D_max	11.9	nm
Volume^Porod	277	nm³

SASDJ74 – P454 peptide from B.pertussis CyaA toxin complexed with calmodulin

Calmodulin-1Bifunctional hemolysin/adenylate cyclase experimental SAS data

Sample:	Calmodulin-1 monomer, 17 kDa Homo sapiens protein Bifunctional hemolysin/adenylate cyclase monomer, 3 kDa Bordetella pertussis protein
Buffer:	20 mM HEPES, 150 mM NaCl, 4 mM CaCl₂, pH: 7.4
Experiment:	SAXS data collected at SWING, SOLEIL on 2016 Sep 24

A High‐Affinity Calmodulin‐Binding Site in the CyaA Toxin Translocation Domain is Essential for Invasion of Eukaryotic Cells Advanced Science :2003630 (2021)
Voegele A, Sadi M, O'Brien D, Gehan P, Raoux‐Barbot D, Davi M, Hoos S, Brûlé S, Raynal B, Weber P, Mechaly A, Haouz A, Rodriguez N, Vachette P, Durand D, Brier S, Ladant D, Chenal A

R_g^Guinier	2.0	nm
D_max	7.0	nm
Volume^Porod	28	nm³

SASDKB8 – Immunoglobulin G1 (IgG1) Glycosylated

Immunoglobulin G subclass 1 experimental SAS data

Sample:	Immunoglobulin G subclass 1, 148 kDa Homo sapiens protein
Buffer:	20 mM L-histidine, 138 mM NaCl, and 2.6 mM KCl buffer, pH: 6
Experiment:	SAXS data collected at B21, Diamond Light Source on 2017 Oct 20

Solution structure of deglycosylated human IgG1 shows the role of CH2 glycans in its conformation Biophysical Journal (2021)
Spiteri V, Doutch J, Rambo R, Gor J, Dalby P, Perkins S

R_g^Guinier	5.1	nm
D_max	17.5	nm
Volume^Porod	269	nm³

SASDKC8 – Immunoglobulin G1 (IgG1) Deglycosylated

Sample:	Immunoglobulin G subclass 1, 148 kDa Homo sapiens protein
Buffer:	20 mM L-histidine, 138 mM NaCl, and 2.6 mM KCl buffer, pH: 6
Experiment:	SAXS data collected at B21, Diamond Light Source on 2017 Oct 20

Solution structure of deglycosylated human IgG1 shows the role of CH2 glycans in its conformation Biophysical Journal (2021)
Spiteri V, Doutch J, Rambo R, Gor J, Dalby P, Perkins S

R_g^Guinier	5.2	nm
D_max	18.4	nm
Volume^Porod	271	nm³

SASDH39 – Complex of Archaeoglobus fulgidus Argonaute with dsDNA (5'p-ATTGTACGTACAAT)

Piwi protein AF_13185'-phosphorilated 14-mer DNA oligoduplex experimental SAS data

Sample:	Piwi protein AF_1318 dimer, 102 kDa Archaeoglobus fulgidus protein 5'-phosphorilated 14-mer DNA oligoduplex dimer, 9 kDa DNA
Buffer:	20 mM TrisHCl, pH 7.5, 5 mM MgCl2, 150 mM NaCl and 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Jul 6

Prokaryotic Argonaute from Archaeoglobus fulgidus interacts with DNA as a homodimer. Sci Rep 11(1):4518 (2021)
Golovinas E, Rutkauskas D, Manakova E, Jankunec M, Silanskas A, Sasnauskas G, Zaremba M

R_g^Guinier	3.2	nm
D_max	10.1	nm
Volume^Porod	158	nm³

SASDH49 – Complex of Archaeoglobus fulgidus Argonaute monomeric mutant with dsDNA (5'p-ATTGTACGTACAAT)

Piwi protein AF_1318 delta (296-303) mutant5'-phosphorilated 14-mer DNA oligoduplex experimental SAS data

Sample:	Piwi protein AF_1318 delta (296-303) mutant monomer, 50 kDa Archaeoglobus fulgidus protein 5'-phosphorilated 14-mer DNA oligoduplex dimer, 9 kDa DNA
Buffer:	20 mM TrisHCl, pH 7.5, 5 mM MgCl2, 150 mM NaCl and 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Jul 6

R_g^Guinier	2.8	nm
D_max	9.6	nm
Volume^Porod	109	nm³

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