Browse by MODEL: Hybrid

SASDJL6 – Sarcomeric F-actin crosslinking protein α-actinin-2 (spectrin repeat rod domain, rod-α-actinin-2)

Rod domain of α-actinin-2 experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Rod domain of α-actinin-2 dimer, 112 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Dec 5
Order from disorder in the sarcomere: FATZ forms a fuzzy but tight complex and phase-separated condensates with α-actinin. Sci Adv 7(22) (2021)
Sponga A, Arolas JL, Schwarz TC, Jeffries CM, Rodriguez Chamorro A, Kostan J, Ghisleni A, Drepper F, Polyansky A, De Almeida Ribeiro E, Pedron M, Zawadzka-Kazimierczuk A, Mlynek G, Peterbauer T, Doto P, Schreiner C, Hollerl E, Mateos B, Geist L, Faulkner G, Kozminski W, Svergun DI, Warscheid B, Zagrovic B, Gautel M, Konrat R, Djinović-Carugo K
RgGuinier 6.7 nm
Dmax 27.2 nm
VolumePorod 214 nm3

SASDJ85 – Zinc transporter 1 – hZnT1-CTD

Zinc transporter 1 experimental SAS data
DAMFILT model
Sample: Zinc transporter 1 dimer, 37 kDa Homo sapiens protein
Buffer: 20mM Tris, 150mM NaCl, 1mM TCEP, pH: 7.6
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Aug 14
Heterologous Expression and Biochemical Characterization of the Human Zinc Transporter 1 (ZnT1) and Its Soluble C-Terminal Domain Frontiers in Chemistry 9 (2021)
Cotrim C, Jarrott R, Whitten A, Choudhury H, Drew D, Martin J
RgGuinier 2.7 nm
Dmax 10.0 nm
VolumePorod 50 nm3

SASDKQ9 – Chaperone protein IpgC, symmetric dimer from Shigella flexneri

Chaperone protein IpgC experimental SAS data
CORAL model
Sample: Chaperone protein IpgC dimer, 39 kDa Shigella flexneri protein
Buffer: 20 mM HEPES, 100 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2012 Dec 10
Structural Insights of Shigella Translocator IpaB and Its Chaperone IpgC in Solution Frontiers in Cellular and Infection Microbiology 11 (2021)
Ferrari M, Charova S, Sansonetti P, Mylonas E, Gazi A
RgGuinier 2.7 nm
Dmax 9.0 nm
VolumePorod 56 nm3

SASDKR9 – The chaperone protein IpgC/invasin IpaB heterodimer complex from Shigella flexneri

T3SS Chaperone protein IpgCInvasin IpaB experimental SAS data
EOM/RANCH model
Sample: T3SS Chaperone protein IpgC monomer, 20 kDa Shigella flexneri protein
Invasin IpaB monomer, 62 kDa Shigella flexneri protein
Buffer: 20 mM HEPES, 100 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2012 Dec 10
Structural Insights of Shigella Translocator IpaB and Its Chaperone IpgC in Solution Frontiers in Cellular and Infection Microbiology 11 (2021)
Ferrari M, Charova S, Sansonetti P, Mylonas E, Gazi A
RgGuinier 3.9 nm
Dmax 14.0 nm
VolumePorod 165 nm3

SASDHV9 – Murine major prion protein PrPc 23-230

Major prion protein experimental SAS data
CORAL model
Sample: Major prion protein monomer, 23 kDa Mus musculus protein
Buffer: 10mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Dec 15
Characterization of murine prion protein
Stefano Da Vela
RgGuinier 2.8 nm
Dmax 9.9 nm
VolumePorod 43 nm3

SASDKM8 – Rationally optimised WA20 (ROWA) dimer

Rationally optimised WA20 mutant N22A/H86K (ROWA) dimer experimental SAS data
DAMMIN model
Sample: Rationally optimised WA20 mutant N22A/H86K (ROWA) dimer dimer, 25 kDa Artificial protein (de … protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5% glycerol,, pH: 7.5
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2019 Nov 30
Rational thermostabilisation of four-helix bundle dimeric de novo proteins. Sci Rep 11(1):7526 (2021)
Irumagawa S, Kobayashi K, Saito Y, Miyata T, Umetsu M, Kameda T, Arai R
RgGuinier 2.5 nm
Dmax 9.9 nm
VolumePorod 30 nm3

SASDKQ8 – Rationally optimised SUWA (ROSA) dimer

Rationally optimised SUWA mutant N22E/H86K (ROSA) dimer experimental SAS data
DAMMIN model
Sample: Rationally optimised SUWA mutant N22E/H86K (ROSA) dimer dimer, 25 kDa Artificial protein (de … protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5% glycerol,, pH: 7.5
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2019 Nov 30
Rational thermostabilisation of four-helix bundle dimeric de novo proteins. Sci Rep 11(1):7526 (2021)
Irumagawa S, Kobayashi K, Saito Y, Miyata T, Umetsu M, Kameda T, Arai R
RgGuinier 2.5 nm
Dmax 10.6 nm
VolumePorod 30 nm3

SASDFB7 – Human phenylalanine hydroxylase (hPAH)

Phenylalanine-4-hydroxylase experimental SAS data
CORAL model
Sample: Phenylalanine-4-hydroxylase tetramer, 223 kDa Homo sapiens protein
Buffer: 0.02 mM Hepes, 0.2 M NaCl,, pH: 7
Experiment: SAXS data collected at B21, Diamond Light Source on 2016 Feb 25
Modulation of Human Phenylalanine Hydroxylase by 3-Hydroxyquinolin-2(1H)-One Derivatives Biomolecules 11(3):462 (2021)
Lopes R, Tomé C, Russo R, Paterna R, Leandro J, Candeias N, Gonçalves L, Teixeira M, Sousa P, Guedes R, Vicente J, Gois P, Leandro P
RgGuinier 4.3 nm
Dmax 14.8 nm
VolumePorod 336 nm3

SASDFC7 – Human phenylalanine hydroxylase (hPAH) + 1 mM L-Phe

Phenylalanine-4-hydroxylase experimental SAS data
DAMMIN model
Sample: Phenylalanine-4-hydroxylase tetramer, 223 kDa Homo sapiens protein
Buffer: 0.02 mM Hepes, 0.2 M NaCl,, pH: 7
Experiment: SAXS data collected at B21, Diamond Light Source on 2016 Feb 25
Modulation of Human Phenylalanine Hydroxylase by 3-Hydroxyquinolin-2(1H)-One Derivatives Biomolecules 11(3):462 (2021)
Lopes R, Tomé C, Russo R, Paterna R, Leandro J, Candeias N, Gonçalves L, Teixeira M, Sousa P, Guedes R, Vicente J, Gois P, Leandro P
RgGuinier 4.0 nm
Dmax 12.3 nm
VolumePorod 367 nm3

SASDKF4 – DNA binding protein of starvation (DPS)

DNA protection during starvation protein experimental SAS data
DAMMIN model
Sample: DNA protection during starvation protein dodecamer, 225 kDa Escherichia coli protein
Buffer: 50 mM Tris-HCl, 50 mM NaCl, 0.5 mM EDTA, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Nov 26
Spatial Organization of Dps and DNA-Dps Complexes. J Mol Biol :166930 (2021)
Dubrovin EV, Dadinova LA, Petoukhov MV, Yu Soshinskaya E, Mozhaev AA, Klinov DV, Schäffer TE, Shtykova EV, Batishchev OV
RgGuinier 3.9 nm
Dmax 14.0 nm
VolumePorod 314 nm3