Browse by MODEL: No model

SASDCQ9 – Ks-Amt5-HK (+10uM ATP)

Ammonium transporter histidine kinase domain experimental SAS data
Ammonium transporter histidine kinase domain Kratky plot
Sample: Ammonium transporter histidine kinase domain monomer, 30 kDa Candidatus Kuenenia stuttgartiensis protein
Buffer: 20 mM Tris/HCl,100 mM NaCl, 5%(w/v) glycerol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Mar 2
Signaling ammonium across membranes through an ammonium sensor histidine kinase. Nat Commun 9(1):164 (2018)
Pflüger T, Hernández CF, Lewe P, Frank F, Mertens H, Svergun D, Baumstark MW, Lunin VY, Jetten MSM, Andrade SLA
RgGuinier 2.2 nm
Dmax 7.7 nm
VolumePorod 53 nm3

SASDCS9 – Ammonium transporter Ks-Amt5 (Batch)

ammonium sensor/transducer experimental SAS data
ammonium sensor/transducer Kratky plot
Sample: Ammonium sensor/transducer trimer, 227 kDa Candidatus Kuenenia stuttgartiensis protein
Buffer: 20 mM Tris/HCl,100 mM NaCl, 5%(w/v) glycerol, 0.09%(w/v) CYMAL-5, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Mar 2
Signaling ammonium across membranes through an ammonium sensor histidine kinase. Nat Commun 9(1):164 (2018)
Pflüger T, Hernández CF, Lewe P, Frank F, Mertens H, Svergun D, Baumstark MW, Lunin VY, Jetten MSM, Andrade SLA
RgGuinier 4.9 nm
Dmax 17.0 nm
VolumePorod 482 nm3

SASDCN7 – Truncated monomeric Cytohesin-3 (Grp1; amino acids 63-399) E161A 6GS Arf6 Q67L His6 fusion protein

Grp1 63-399 E161A 6GS Arf6 Q67L His fusion protein experimental SAS data
Grp1 63-399 E161A 6GS Arf6 Q67L His fusion protein Kratky plot
Sample: Grp1 63-399 E161A 6GS Arf6 Q67L His fusion protein monomer, 62 kDa Mus musculus protein
Buffer: 20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 15
Structural Dynamics Control Allosteric Activation of Cytohesin Family Arf GTPase Exchange Factors. Structure 26(1):106-117.e6 (2018)
Malaby AW, Das S, Chakravarthy S, Irving TC, Bilsel O, Lambright DG
RgGuinier 3.4 nm
Dmax 13.6 nm
VolumePorod 101 nm3

SASDCT9 – Bacteriophage phi-X174 - Empty Capsid

Bacteriophage phi-X174 experimental SAS data
Bacteriophage phi-X174 Kratky plot
Sample: Bacteriophage phi-X174 monomer, 0 kDa protein
Buffer: 0.06 M NH4Cl2, 0.09 M NaCl, 0.1 M KCl, 1 mM MgS04, 1 mM CaCl2, 0.1 M Tris-HCl, pH: 7.4
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25
Structural changes of tailless bacteriophage ΦX174 during penetration of bacterial cell walls. Proc Natl Acad Sci U S A 114(52):13708-13713 (2017)
Sun Y, Roznowski AP, Tokuda JM, Klose T, Mauney A, Pollack L, Fane BA, Rossmann MG
RgGuinier 14.0 nm

SASDCU9 – Bacteriophage phi-X174 - Before Reaction with LPS

Bacteriophage phi-X174 experimental SAS data
Bacteriophage phi-X174 Kratky plot
Sample: Bacteriophage phi-X174 monomer, 0 kDa protein
Buffer: 0.06 M NH4Cl2, 0.09 M NaCl, 0.1 M KCl, 1 mM MgS04, 1 mM CaCl2, 0.1 M Tris-HCl, pH: 7.4
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25
Structural changes of tailless bacteriophage ΦX174 during penetration of bacterial cell walls. Proc Natl Acad Sci U S A 114(52):13708-13713 (2017)
Sun Y, Roznowski AP, Tokuda JM, Klose T, Mauney A, Pollack L, Fane BA, Rossmann MG
RgGuinier 12.1 nm

SASDCV9 – Bacteriophage phi-X174 - ~45s after mixing with LPS

Bacteriophage phi-X174 experimental SAS data
Bacteriophage phi-X174 Kratky plot
Sample: Bacteriophage phi-X174 monomer, 0 kDa protein
Buffer: 0.15 mg/mL LPS, 0.06 M NH4Cl2, 0.09 M NaCl, 0.1 M KCl, 1 mM MgS04, 1 mM CaCl2, 0.1 M Tris-HCl, pH: 7.4
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25
Structural changes of tailless bacteriophage ΦX174 during penetration of bacterial cell walls. Proc Natl Acad Sci U S A 114(52):13708-13713 (2017)
Sun Y, Roznowski AP, Tokuda JM, Klose T, Mauney A, Pollack L, Fane BA, Rossmann MG
RgGuinier 12.7 nm

SASDCW9 – Bacteriophage phi-X174 - ~245s after mixing with LPS

Bacteriophage phi-X174 experimental SAS data
Bacteriophage phi-X174 Kratky plot
Sample: Bacteriophage phi-X174 monomer, 0 kDa protein
Buffer: 0.15 mg/mL LPS, 0.06 M NH4Cl2, 0.09 M NaCl, 0.1 M KCl, 1 mM MgS04, 1 mM CaCl2, 0.1 M Tris-HCl, pH: 7.4
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25
Structural changes of tailless bacteriophage ΦX174 during penetration of bacterial cell walls. Proc Natl Acad Sci U S A 114(52):13708-13713 (2017)
Sun Y, Roznowski AP, Tokuda JM, Klose T, Mauney A, Pollack L, Fane BA, Rossmann MG
RgGuinier 12.9 nm

SASDCX9 – Bacteriophage phi-X174 - ~545s after mixing with LPS

Bacteriophage phi-X174 experimental SAS data
Bacteriophage phi-X174 Kratky plot
Sample: Bacteriophage phi-X174 monomer, 0 kDa protein
Buffer: 0.15 mg/mL LPS, 0.06 M NH4Cl2, 0.09 M NaCl, 0.1 M KCl, 1 mM MgS04, 1 mM CaCl2, 0.1 M Tris-HCl, pH: 7.4
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25
Structural changes of tailless bacteriophage ΦX174 during penetration of bacterial cell walls. Proc Natl Acad Sci U S A 114(52):13708-13713 (2017)
Sun Y, Roznowski AP, Tokuda JM, Klose T, Mauney A, Pollack L, Fane BA, Rossmann MG
RgGuinier 12.9 nm

SASDCB7 – Streptococcus pneumoniae phosphodiesterase Pde2

DHH subfamily 1 protein experimental SAS data
DHH subfamily 1 protein Kratky plot
Sample: DHH subfamily 1 protein dimer, 70 kDa Streptococcus pneumoniae serotype … protein
Buffer: 20mM Tris, 200 mM NaCl, 5%(v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jun 23
Structural and Biophysical Analysis of the Soluble DHH/DHHA1-Type Phosphodiesterase TM1595 from Thermotoga maritima. Structure 25(12):1887-1897.e4 (2017)
Drexler DJ, Müller M, Rojas-Cordova CA, Bandera AM, Witte G
RgGuinier 2.7 nm
Dmax 7.7 nm
VolumePorod 87 nm3

SASDCC7 – Thermotoga martitima phosphodiesterase TM1595 D80N D154N (inactive mutant)

T.maritima PDE experimental SAS data
T.maritima PDE Kratky plot
Sample: T.maritima PDE dimer, 76 kDa Thermotoga maritima protein
Buffer: 25mM Tris 500mM NaCl 3% (v/v) glycerol 2mM MgCl2, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Jun 17
Structural and Biophysical Analysis of the Soluble DHH/DHHA1-Type Phosphodiesterase TM1595 from Thermotoga maritima. Structure 25(12):1887-1897.e4 (2017)
Drexler DJ, Müller M, Rojas-Cordova CA, Bandera AM, Witte G
RgGuinier 2.8 nm
Dmax 7.9 nm
VolumePorod 115 nm3