Small Angle Scattering Biological Data Bank SASBDB

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SASDWD4 – 20 nm polystyrene beads from AF4-SAXS measurements

Thermo Fisher polystyrene nanospheres with a diameter of 20 nm experimental SAS data

Thermo Fisher polystyrene nanospheres with a diameter of 20 nm Kratky plot

Sample:	Thermo Fisher polystyrene nanospheres with a diameter of 20 nm monomer, 1 kDa synthetic construct
Buffer:	water/detergent solution (0.0125 % (v/v) NovaChem), pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2023 Jun 19

AF4-to-SAXS: expanded characterization of nanoparticles and proteins at the P12 BioSAXS beamline. J Synchrotron Radiat (2025)
Da Vela S, Bartels K, Franke D, Soloviov D, Gräwert T, Molodenskiy D, Kolb B, Wilhelmy C, Drexel R, Meier F, Haas H, Langguth P, Graewert MA

R_g^Guinier	9.0	nm
D_max	25.0	nm

SASDV57 – Full-length SFPQ in high salt buffer

Splicing factor, proline- and glutamine-rich experimental SAS data

Splicing factor, proline- and glutamine-rich Kratky plot

Sample:	Splicing factor, proline- and glutamine-rich dimer, 153 kDa Homo sapiens protein
Buffer:	500mM KNO3, 20mM HEPES, 5% glycerol, 1mM DTT, pH: 7.4
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Oct 22

Structural dynamics of IDR interactions in human SFPQ and implications for liquid–liquid phase separation Acta Crystallographica Section D Structural Biology 81(7):357-379 (2025)
Koning H, Lai V, Sethi A, Chakraborty S, Ang C, Fox A, Duff A, Whitten A, Marshall A, Bond C

R_g^Guinier	7.9	nm
D_max	42.5	nm
Volume^Porod	485	nm³

SASDV67 – Human Splicing Factor Proline/Glutamine rich SFPQ1 dimer with C-terminal truncation (delta599-707)

Splicing Factor Proline/Glutamine rich (1-598) experimental SAS data

Splicing Factor Proline/Glutamine rich (1-598) Kratky plot

Sample:	Splicing Factor Proline/Glutamine rich (1-598) dimer, 130 kDa Homo sapiens protein
Buffer:	500mM KNO3, 20mM HEPES, 5% glycerol, 1mM DTT, pH: 7.4
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2023 Mar 13

R_g^Guinier	7.6	nm
D_max	34.3	nm
Volume^Porod	400	nm³

SASDV77 – Human Splicing Factor Proline/Glutamine rich SFPQ1 dimer with C-terminal truncation (delta599-707) in low salt buffer

Sample:	Splicing Factor Proline/Glutamine rich (1-598) dimer, 130 kDa Homo sapiens protein
Buffer:	150 mM KCl, 20 mM HEPES, 5% glycerol, 5 mM MgCl2, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2023 Mar 13

R_g^Guinier	6.1	nm
D_max	28.0	nm
Volume^Porod	315	nm³

SASDV59 – Human Splicing factor, proline- and glutamine-rich protein 5% SFPQ and 95% dSFPQ at a D2O matchpoint of 95%

Sample:	Splicing factor, proline- and glutamine-rich dimer, 153 kDa Homo sapiens protein
Buffer:	150 mM KCl, 0.075% glycerol, 20 mM HEPES, 0.5 mM DTT,, pH: 7.4
Experiment:	SANS data collected at Quokka - Small Angle Neutron Scattering, Australian Centre for Neutron Scattering (ANSTO) on 2023 Jan 20

R_g^Guinier	6.1	nm
D_max	22.0	nm
Volume^Porod	181	nm³

SASDXD4 – Full-length human SFPQ dimer (protiated and deuterated)

Sample:	Splicing factor, proline- and glutamine-rich dimer, 153 kDa Homo sapiens protein
Buffer:	150 mM KCl, 1.5% glycerol, 20 mM HEPES, 1 mM DTT (in 100% H2O), pH: 7.4
Experiment:	SANS data collected at Quokka - Small Angle Neutron Scattering, Australian Centre for Neutron Scattering (ANSTO) on 2023 Jan 23

R_g^Guinier	8.7	nm
D_max	30.7	nm
Volume^Porod	241000	nm³

SASDSC6 – Human Ubiquilin-2, ∆STI1-1 domain deletion (UBQLN2∆STI1-1)

Ubiquilin-2 (∆170-260) experimental SAS data

Sample:	Ubiquilin-2 (∆170-260) dimer, 110 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 16

UBQLN2 domain deletion constructs
Sarasi Galagedera

R_g^Guinier	5.4	nm
D_max	26.0	nm
Volume^Porod	357	nm³

SASDSD6 – Human Ubiquilin-2 with ∆UBL and ∆STI1-1 domain deletions (UBQLN2∆STI1-1∆UBL)

Ubiquilin-2 (∆33-103, ∆170-260) experimental SAS data

Ubiquilin-2 (∆33-103, ∆170-260) Kratky plot

Sample:	Ubiquilin-2 (∆33-103, ∆170-260) dimer, 94 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 16

UBQLN2 domain deletion constructs
Sarasi Galagedera

R_g^Guinier	5.6	nm
D_max	24.5	nm
Volume^Porod	326	nm³

SASDSE6 – Human Ubiquilin-2, ∆1-108 N-terminal deletion (UBQLN2 109-624)

Ubiquilin-2 (∆1-108) experimental SAS data

Sample:	Ubiquilin-2 (∆1-108) dimer, 108 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 16

UBQLN2 domain deletion constructs
Sarasi Galagedera

R_g^Guinier	5.2	nm
D_max	23.3	nm
Volume^Porod	281	nm³

SASDSF6 – Human Ubiquilin-2, C-terminal construct (UBQLN2 450-624W)

Ubiquilin-2 (∆1-449)-W experimental SAS data

Sample:	Ubiquilin-2 (∆1-449)-W dimer, 35 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 16

UBQLN2 domain deletion constructs
Sarasi Galagedera

R_g^Guinier	3.8	nm
D_max	18.5	nm
Volume^Porod	50	nm³

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