Small Angle Scattering Biological Data Bank SASBDB

MACROMOLECULE TYPE: heterocomplex

SASDRA4 – GAC rRNA + L11 Protein: Time-Resolved 50 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA50S ribosomal protein L11 experimental SAS data

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA 50S ribosomal protein L11 Kratky plot

Sample:	58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA 50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer:	10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
Zielinski K, Katz A, Calvey G, Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L

R_g^Guinier	2.5	nm
D_max	13.5	nm
Volume^Porod	36	nm³

SASDRB4 – GAC rRNA + L11 Protein: Time-Resolved 63 ms

Sample:	58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA 50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer:	10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

R_g^Guinier	2.4	nm
D_max	11.5	nm
Volume^Porod	33	nm³

SASDRC4 – GAC rRNA + L11 Protein: Time-Resolved 100 ms

Sample:	58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA 50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer:	10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

R_g^Guinier	2.4	nm
D_max	11.5	nm
Volume^Porod	35	nm³

SASDRD4 – GAC rRNA + L11 Protein: Time-Resolved 200 ms

Sample:	58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA 50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer:	10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

R_g^Guinier	2.5	nm
D_max	12.5	nm
Volume^Porod	37	nm³

SASDRE4 – GAC rRNA + L11 Protein: Time-Resolved 316 ms

Sample:	58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA 50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer:	10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

R_g^Guinier	2.5	nm
D_max	10.0	nm
Volume^Porod	38	nm³

SASDRF4 – GAC rRNA + L11 Protein: Time-Resolved 631 ms

Sample:	58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA 50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer:	10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

R_g^Guinier	2.5	nm
D_max	13.0	nm
Volume^Porod	38	nm³

SASDRG4 – GAC rRNA + L11 Protein: Time-Resolved 1000 ms

Sample:	58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA 50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer:	10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

R_g^Guinier	2.5	nm
D_max	12.5	nm
Volume^Porod	40	nm³

SASDRH4 – GAC rRNA + L11 Protein: Time-Resolved 2000 ms

Sample:	58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA 50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer:	10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

R_g^Guinier	2.6	nm
D_max	12.8	nm
Volume^Porod	39	nm³

SASDRJ4 – GAC rRNA + L11 Protein: SEC-SAXS final

Sample:	58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA 50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer:	10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Nov 19

R_g^Guinier	2.6	nm
D_max	13.0	nm
Volume^Porod	48	nm³

SASDQ79 – Mutant 7-deazapurine in DNA protein A (D95A) bound to a 28 base pair DNA substrate (2-state model: complex plus free DNA)

Custom 28 base pair double stranded DNADNA-guanine transglycosylase - D95A mutant experimental SAS data

Sample:	Custom 28 base pair double stranded DNA monomer, 17 kDa Synthetic, purchased from … DNA DNA-guanine transglycosylase - D95A mutant monomer, 50 kDa Salmonella enterica subsp. … protein
Buffer:	100 mM KCl, 50 mM Tris pH 7.0, 1 mM DTT, pH: 7
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 Dec 22

7-Deazaguanines in DNA: functional and structural elucidation of a DNA modification system. Nucleic Acids Res (2023)
Gedara SH, Wood E, Gustafson A, Liang C, Hung SH, Savage J, Phan P, Luthra A, de Crécy-Lagard V, Dedon P, Swairjo MA, Iwata-Reuyl D

R_g^Guinier	3.4	nm
D_max	13.5	nm
Volume^Porod	104	nm³

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