Small Angle Scattering Biological Data Bank SASBDB

Browse by MACROMOLECULE type: nucleic acid

SASDCK4 – Truncated P5abc subdomain from tetrahymena ribozyme: Static 0.25mM MgCl2

Truncated P5abc subdomain from tetrahymena ribozyme experimental SAS data

Truncated P5abc subdomain from tetrahymena ribozyme Kratky plot

Sample:	Truncated P5abc subdomain from tetrahymena ribozyme monomer, 18 kDa RNA
Buffer:	20mM KCl 0.25mM MgCl2 10mM KMOPS 20uM EDTA, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Jun 17

Revealing the distinct folding phases of an RNA three-helix junction. Nucleic Acids Res 46(14):7354-7365 (2018)
Plumridge A, Katz AM, Calvey GD, Elber R, Kirmizialtin S, Pollack L

R_g^Guinier	2.4	nm
D_max	7.6	nm

SASDCL4 – Truncated P5abc subdomain from tetrahymena ribozyme: Static 0.50mM MgCl2

Sample:	Truncated P5abc subdomain from tetrahymena ribozyme monomer, 18 kDa RNA
Buffer:	0.5mM MgCl2 20mM KCl 10mM KMOPS 20uM EDTA, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Jun 17

Revealing the distinct folding phases of an RNA three-helix junction. Nucleic Acids Res 46(14):7354-7365 (2018)
Plumridge A, Katz AM, Calvey GD, Elber R, Kirmizialtin S, Pollack L

R_g^Guinier	2.3	nm
D_max	7.2	nm

SASDCM4 – Truncated P5abc subdomain from tetrahymena ribozyme: Static 1mM MgCl2

Sample:	Truncated P5abc subdomain from tetrahymena ribozyme monomer, 18 kDa RNA
Buffer:	1mM MgCl2 20mM KCl 10mM KMOPS 20uM EDTA, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Jun 17

Revealing the distinct folding phases of an RNA three-helix junction. Nucleic Acids Res 46(14):7354-7365 (2018)
Plumridge A, Katz AM, Calvey GD, Elber R, Kirmizialtin S, Pollack L

R_g^Guinier	2.2	nm
D_max	7.2	nm

SASDDF7 – Armless mitochondrial tRNA-Arg from Romanomermis culicivorax

Arginyl transfer RNA experimental SAS data

Sample:	Arginyl transfer RNA monomer, 13 kDa Romanomermis culicivorax RNA
Buffer:	50 mM HEPES-NaOH 10 mM MgCl2, pH: 7.4
Experiment:	SAXS data collected at SWING, SOLEIL on 2015 Dec 16

Small but large enough: structural properties of armless mitochondrial tRNAs from the nematode Romanomermis culicivorax. Nucleic Acids Res (2018)
Jühling T, Duchardt-Ferner E, Bonin S, Wöhnert J, Pütz J, Florentz C, Betat H, Sauter C, Mörl M

R_g^Guinier	2.1	nm
D_max	6.7	nm
Volume^Porod	20	nm³

SASDDG7 – Armless mitochondrial tRNA-Ile from Romanomermis culicivorax

Isoleucyl transfer RNA experimental SAS data

Sample:	Isoleucyl transfer RNA monomer, 15 kDa Romanomermis culicivorax RNA
Buffer:	50 mM HEPES-NaOH 10 mM MgCl2, pH: 7.4
Experiment:	SAXS data collected at SWING, SOLEIL on 2015 Dec 16

R_g^Guinier	2.0	nm
D_max	6.7	nm
Volume^Porod	20	nm³

SASDCC8 – Human TERC 1-20 DNA G-quadruplex

Human telomerase RNA template component, DNA counterpart, nucleotides 1-20 experimental SAS data

Sample:	Human telomerase RNA template component, DNA counterpart, nucleotides 1-20 dimer, 13 kDa Homo sapiens DNA
Buffer:	20 mM HEPES, 100 mM KCl,, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2017 Aug 2

Structure and hydrodynamics of a DNA G-quadruplex with a cytosine bulge. Nucleic Acids Res 46(10):5319-5331 (2018)
Meier M, Moya-Torres A, Krahn NJ, McDougall MD, Orriss GL, McRae EKS, Booy EP, McEleney K, Patel TR, McKenna SA, Stetefeld J

R_g^Guinier	1.4	nm
D_max	4.6	nm
Volume^Porod	15	nm³

SASDC95 – Light encoded DNA biosensor: e14A DNA

Sample:	E14A monomer, 21 kDa DNA
Buffer:	154 mM NaCl, pH: 8.3
Experiment:	SAXS data collected at BM29, ESRF on 2016 Jun 16

Optical and Structural Characterization of a Chronic Myeloid Leukemia DNA Biosensor. ACS Chem Biol 13(5):1235-1242 (2018)
Cordeiro M, Otrelo-Cardoso AR, Svergun DI, Konarev PV, Lima JC, Santos-Silva T, Baptista PV

R_g^Guinier	2.8	nm
D_max	12.5	nm

SASDCA5 – Light encoded DNA biosensor: e14B DNA

Sample:	E14B monomer, 13 kDa DNA
Buffer:	154 mM NaCl, pH: 8.3
Experiment:	SAXS data collected at BM29, ESRF on 2016 Jun 16

R_g^Guinier	1.9	nm
D_max	9.0	nm

SASDCB5 – Light encoded DNA biosensor: e14C DNA

Sample:	E14C monomer, 5 kDa DNA
Buffer:	154 mM NaCl, pH: 8.3
Experiment:	SAXS data collected at BM29, ESRF on 2016 Jun 16

R_g^Guinier	1.3	nm
D_max	5.0	nm

SASDCC5 – Light encoded DNA biosensor: e14AB DNA

Sample:	E14AB monomer, 93 kDa DNA
Buffer:	154 mM NaCl, pH: 8.3
Experiment:	SAXS data collected at BM29, ESRF on 2016 Jun 16

R_g^Guinier	5.4	nm
D_max	24.7	nm

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