Small Angle Scattering Biological Data Bank SASBDB

Browse by MACROMOLECULE type: protein

SASDS75 – HIV-1 Reverse Transcriptase monomer (p51 subunit with W401A mutation) unliganded form

Gag-Pol polyprotein (588-1027: W988A mutant; Reverse transcriptase p51; p51 RT) experimental SAS data

Gag-Pol polyprotein (588-1027: W988A mutant; Reverse transcriptase p51; p51 RT) Kratky plot

Sample:	Gag-Pol polyprotein (588-1027: W988A mutant; Reverse transcriptase p51; p51 RT) monomer, 53 kDa Human immunodeficiency virus … protein
Buffer:	20 mM Tris-HCl, 100 mM NaCl, 1 mM TCEP, pH: 7
Experiment:	SAXS data collected at BL1.3W, Synchrotron Light Research Institute (SLRI) on 2017 Jun 19

Biophysical Characterization of p51 and p66 Monomers of HIV-1 Reverse Transcriptase with Their Inhibitors. Protein J (2023)
Seetaha S, Kamonsutthipaijit N, Yagi-Utsumi M, Seako Y, Yamaguchi T, Hannongbua S, Kato K, Choowongkomon K

R_g^Guinier	2.8	nm
D_max	9.8	nm
Volume^Porod	84	nm³

SASDS85 – HIV-1 Reverse Transcriptase monomer (p51 subunit with W401A mutation) bound with Nevirapine

Sample:	Gag-Pol polyprotein (588-1027: W988A mutant; Reverse transcriptase p51; p51 RT) monomer, 53 kDa Human immunodeficiency virus … protein
Buffer:	20 mM Tris-HCl, 100 mM NaCl, 1 mM TCEP, pH: 7
Experiment:	SAXS data collected at BL1.3W, Synchrotron Light Research Institute (SLRI) on 2017 Jun 19

R_g^Guinier	3.1	nm
D_max	11.1	nm
Volume^Porod	103	nm³

SASDS95 – HIV-1 Reverse Transcriptase monomer (p51 subunit with W401A mutation) bound with Efavirenz

Sample:	Gag-Pol polyprotein (588-1027: W988A mutant; Reverse transcriptase p51; p51 RT) monomer, 53 kDa Human immunodeficiency virus … protein
Buffer:	20 mM Tris-HCl, 100 mM NaCl, 1 mM TCEP, pH: 7
Experiment:	SAXS data collected at BL1.3W, Synchrotron Light Research Institute (SLRI) on 2017 Jun 19

R_g^Guinier	4.2	nm
D_max	16.7	nm
Volume^Porod	226	nm³

SASDQQ8 – Endo-D-arabinanase, 54 kDa, dimer, Microbacterium arabinogalactanolyticum

Endo-D-arabinanase experimental SAS data

Sample:	Endo-D-arabinanase dimer, 107 kDa Microbacterium arabinogalactanolyticum protein
Buffer:	20 mM Tris-HCl, 200 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2022 Nov 3

Identification and characterization of endo-α-, exo-α-, and exo-β-d-arabinofuranosidases degrading lipoarabinomannan and arabinogalactan of mycobacteria Nature Communications 14(1) (2023)
Shimokawa M, Ishiwata A, Kashima T, Nakashima C, Li J, Fukushima R, Sawai N, Nakamori M, Tanaka Y, Kudo A, Morikami S, Iwanaga N, Akai G, Shimizu N, Arakawa T, Yamada C, Kitahara K, Tanaka K, Ito Y, Fushinobu S, Fujita K

R_g^Guinier	3.1	nm
D_max	10.4	nm
Volume^Porod	131	nm³

SASDRJ8 – A de novo enzyme, ferric enterobactin esterase Syn-F4

De novo enterobactin esterase Syn-F4 (K4T) experimental SAS data

Sample:	De novo enterobactin esterase Syn-F4 (K4T) dimer, 25 kDa synthetic construct protein
Buffer:	25 mM MES, 100 mM NaCl, 10% glycerol, 200 mM Arg-HCl, pH: 6.5
Experiment:	SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2018 Feb 11

Crystal structure and activity of a de novo enzyme, ferric enterobactin esterase Syn-F4. Proc Natl Acad Sci U S A 120(38):e2218281120 (2023)
Kurihara K, Umezawa K, Donnelly AE, Sperling B, Liao G, Hecht MH, Arai R

R_g^Guinier	2.5	nm
D_max	10.0	nm
Volume^Porod	40	nm³

SASDN98 – SvGT under calcium free conditions: Apo state

Glyco_trans_2-like domain-containing protein experimental SAS data

Sample:	Glyco_trans_2-like domain-containing protein dimer, 96 kDa Streptomyces sp. M41(2017) protein
Buffer:	20 mM Tris, pH 7.5, 100 mM NaCl, and 2 mM DTT + 1 mM EDTA, pH: 7.5
Experiment:	SAXS data collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) on 2019 Oct 18

Global shape of SvGT, a metal-dependent bacteriocin modifying S/O-HexNActransferase from actinobacteria: c -terminal dimerization modulates the function of this GT. J Biomol Struct Dyn :1-15 (2023)
Sharma Y, Ahlawat S, Ashish, Rao A

R_g^Guinier	4.4	nm
D_max	17.0	nm
Volume^Porod	151	nm³

SASDNA8 – SvGT under calcium free conditions: Mg2+ bound state

Sample:	Glyco_trans_2-like domain-containing protein dimer, 96 kDa Streptomyces sp. M41(2017) protein
Buffer:	20 mM Tris, pH 7.5, 100 mM NaCl, 2 mM DTT, 1 mM EDTA, 2 mM MgCl2, pH: 7.5
Experiment:	SAXS data collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) on 2019 Oct 18

R_g^Guinier	5.8	nm
D_max	17.9	nm
Volume^Porod	111	nm³

SASDNB8 – Mature full length human prion protein

Major prion protein experimental SAS data

Mature full length human prion protein Rg histogram

Sample:	Major prion protein monomer, 23 kDa Homo sapiens protein
Buffer:	10 mM HEPES 100 mM NaCl, pH: 7
Experiment:	SAXS data collected at BM29, ESRF on 2020 Dec 13

A tetracationic porphyrin with dual anti-prion activity. iScience 26(9):107480 (2023)
Masone A, Zucchelli C, Caruso E, Lavigna G, Eraña H, Giachin G, Tapella L, Comerio L, Restelli E, Raimondi I, Elezgarai SR, De Leo F, Quilici G, Taiarol L, Oldrati M, Lorenzo NL, García-Martínez S, Cagnotto A, Lucchetti J, Gobbi M, Vanni I, Nonno R, Di Bari MA, Tully MD, Cecatiello V, Ciossani G, Pasqualato S, Van Anken E, Salmona M, Castilla J, Requena JR, Banfi S, Musco G, Chiesa R

R_g^Guinier	2.8	nm
D_max	10.2	nm

SASDNC8 – Mature full length human prion protein in the presence of zinc-tetracationic porphyrin [Zn(II)-BnPyP]

Mature full length human prion protein in the presence of zinc-tetracationic porphyrin [Zn(II)-BnPyP] Rg histogram

Sample:	Major prion protein monomer, 23 kDa Homo sapiens protein
Buffer:	10 mM HEPES 100 mM NaCl, pH: 7
Experiment:	SAXS data collected at BM29, ESRF on 2020 Dec 13

R_g^Guinier	3.1	nm
D_max	11.3	nm

SASDND8 – Mature full length human prion protein in the presence of a cationic Fe(III) porphyrin [Fe(III)-TMPyP]

Mature full length human prion protein in the presence of a cationic Fe(III) porphyrin [Fe(III)-TMPyP] Rg histogram

Sample:	Major prion protein monomer, 23 kDa Homo sapiens protein
Buffer:	10 mM HEPES 100 mM NaCl, pH: 7
Experiment:	SAXS data collected at BM29, ESRF on 2020 Dec 13

R_g^Guinier	2.8	nm
D_max	10.3	nm

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