Small Angle Scattering Biological Data Bank SASBDB

Browse by MACROMOLECULE type: protein

SASDR55 – A complex between the periplasmic domains of cholera toxin transcriptional activator ToxR and transmembrane regulatory protein ToxS bound to bile salt

Cholera toxin transcriptional activatorTransmembrane regulatory protein ToxSbile acid: sodium cholate hydrate experimental SAS data

Sample:	Cholera toxin transcriptional activator monomer, 12 kDa Vibrio cholerae serotype … protein Transmembrane regulatory protein ToxS dimer, 37 kDa Vibrio cholerae serotype … protein Bile acid: sodium cholate hydrate monomer, 0 kDa
Buffer:	50 mM Na2HPO4, 300 mM NaCl, 3% glycerol, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2022 Jul 7

Vibrio cholerae's ToxRS bile sensing system. Elife 12 (2023)
Gubensäk N, Sagmeister T, Buhlheller C, Geronimo BD, Wagner GE, Petrowitsch L, Gräwert MA, Rotzinger M, Berger TMI, Schäfer J, Usón I, Reidl J, Sánchez-Murcia PA, Zangger K, Pavkov-Keller T

R_g^Guinier	2.4	nm
D_max	6.6	nm
Volume^Porod	60	nm³

SASDSG6 – Human Ubiquilin-2, ∆STI1-2 domain deletion (UBQLN2∆STI1-2)

Ubiquilin-2 (∆379-462) experimental SAS data

Sample:	Ubiquilin-2 (∆379-462) monomer, 56 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jun 8

Short disordered N-termini & proline-rich domain are major regulators of UBQLN1/2/4 phase separation.
Sarasi Galagedera

R_g^Guinier	4.4	nm
D_max	22.4	nm
Volume^Porod	121	nm³

SASDSH6 – Human Ubiquilin-2, PXX domain deletion (UBQLN2∆PXX)

Ubiquilin-2 (∆487-538) experimental SAS data

Sample:	Ubiquilin-2 (∆487-538) dimer, 122 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jun 8

Short disordered N-termini & proline-rich domain are major regulators of UBQLN1/2/4 phase separation.
Sarasi Galagedera

R_g^Guinier	4.8	nm
D_max	19.5	nm
Volume^Porod	288	nm³

SASDRQ8 – Bovine serum albumin monomer separated using asymmetric flow field flow fractionation (AFFFF)

Sample:	Albumin monomer, 66 kDa Bos taurus protein
Buffer:	10 mM HEPES, 5 mM NaCl, 0.1 mM EDTA, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2022 Jun 8

Quantitative size-resolved characterization of mRNA nanoparticles by in-line coupling of asymmetrical-flow field-flow fractionation with small angle X-ray scattering. Sci Rep 13(1):15764 (2023)
Graewert MA, Wilhelmy C, Bacic T, Schumacher J, Blanchet C, Meier F, Drexel R, Welz R, Kolb B, Bartels K, Nawroth T, Klein T, Svergun D, Langguth P, Haas H

R_g^Guinier	2.9	nm
D_max	9.0	nm
Volume^Porod	104	nm³

SASDSZ4 – HIV-1 Reverse Transcriptase heterodimer (p66/p51) unliganded form

Gag-Pol polyprotein (588-1147: Reverse transcriptase/ribonuclease H; p66)Gag-Pol polyprotein (588-1027: Reverse transcriptase p51; p51 RT) experimental SAS data

Gag-Pol polyprotein (588-1147: Reverse transcriptase/ribonuclease H; p66) Gag-Pol polyprotein (588-1027: Reverse transcriptase p51; p51 RT) Kratky plot

Sample:	Gag-Pol polyprotein (588-1147: Reverse transcriptase/ribonuclease H; p66) monomer, 67 kDa Human immunodeficiency virus … protein Gag-Pol polyprotein (588-1027: Reverse transcriptase p51; p51 RT) monomer, 53 kDa Human immunodeficiency virus … protein
Buffer:	20 mM Tris-HCl, 100 mM NaCl, 1 mM TCEP, pH: 7
Experiment:	SAXS data collected at BL1.3W, Synchrotron Light Research Institute (SLRI) on 2017 Jun 19

Biophysical Characterization of p51 and p66 Monomers of HIV-1 Reverse Transcriptase with Their Inhibitors. Protein J (2023)
Seetaha S, Kamonsutthipaijit N, Yagi-Utsumi M, Seako Y, Yamaguchi T, Hannongbua S, Kato K, Choowongkomon K

R_g^Guinier	3.3	nm
D_max	9.9	nm
Volume^Porod	153	nm³

SASDS25 – HIV-1 Reverse Transcriptase heterodimer (p66/p51) bound with Nevirapine

Sample:	Gag-Pol polyprotein (588-1147: Reverse transcriptase/ribonuclease H; p66) monomer, 67 kDa Human immunodeficiency virus … protein Gag-Pol polyprotein (588-1027: Reverse transcriptase p51; p51 RT) monomer, 53 kDa Human immunodeficiency virus … protein
Buffer:	20 mM Tris-HCl, 100 mM NaCl, 1 mM TCEP, pH: 7
Experiment:	SAXS data collected at BL1.3W, Synchrotron Light Research Institute (SLRI) on 2017 Jun 19

R_g^Guinier	3.5	nm
D_max	10.9	nm
Volume^Porod	161	nm³

SASDS35 – HIV-1 Reverse Transcriptase monomer (p66 subunit with W401A mutation) unliganded form

Gag-Pol polyprotein (588-1147: W988A mutant; Reverse transcriptase/ribonuclease H; p66) experimental SAS data

Gag-Pol polyprotein (588-1147: W988A mutant; Reverse transcriptase/ribonuclease H; p66) Kratky plot

Sample:	Gag-Pol polyprotein (588-1147: W988A mutant; Reverse transcriptase/ribonuclease H; p66) monomer, 67 kDa Human immunodeficiency virus … protein
Buffer:	20 mM Tris-HCl, 100 mM NaCl, 1 mM TCEP, pH: 7
Experiment:	SAXS data collected at BL1.3W, Synchrotron Light Research Institute (SLRI) on 2017 Jun 19

R_g^Guinier	3.1	nm
D_max	10.7	nm
Volume^Porod	98	nm³

SASDS45 – HIV-1 Reverse Transcriptase heterodimer (p66/p51) bound with Efavirenz

Sample:	Gag-Pol polyprotein (588-1147: Reverse transcriptase/ribonuclease H; p66) monomer, 67 kDa Human immunodeficiency virus … protein Gag-Pol polyprotein (588-1027: Reverse transcriptase p51; p51 RT) monomer, 53 kDa Human immunodeficiency virus … protein
Buffer:	20 mM Tris-HCl, 100 mM NaCl, 1 mM TCEP, pH: 7
Experiment:	SAXS data collected at BL1.3W, Synchrotron Light Research Institute (SLRI) on 2017 Jun 19

R_g^Guinier	3.7	nm
D_max	11.3	nm
Volume^Porod	178	nm³

SASDS55 – HIV-1 Reverse Transcriptase monomer (p66 subunit with W401A mutation) bound with Nevirapine

Sample:	Gag-Pol polyprotein (588-1147: W988A mutant; Reverse transcriptase/ribonuclease H; p66) monomer, 67 kDa Human immunodeficiency virus … protein
Buffer:	20 mM Tris-HCl, 100 mM NaCl, 1 mM TCEP, pH: 7
Experiment:	SAXS data collected at BL1.3W, Synchrotron Light Research Institute (SLRI) on 2017 Jun 19

R_g^Guinier	3.5	nm
D_max	11.3	nm
Volume^Porod	134	nm³

SASDS65 – HIV-1 Reverse Transcriptase monomer (p66 subunit with W401A mutation) bound with Efavirenz

Sample:	Gag-Pol polyprotein (588-1147: W988A mutant; Reverse transcriptase/ribonuclease H; p66) monomer, 67 kDa Human immunodeficiency virus … protein
Buffer:	20 mM Tris-HCl, 100 mM NaCl, 1 mM TCEP, pH: 7
Experiment:	SAXS data collected at BL1.3W, Synchrotron Light Research Institute (SLRI) on 2017 Jun 19

R_g^Guinier	4.6	nm
D_max	15.0	nm
Volume^Porod	270	nm³

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