Browse by MACROMOLECULE type: protein

SASDRC6 – Dual specificity mitogen-activated protein kinase kinase 6 (MKK6GRA) bound to mitogen-activated protein kinase 14 (p38α) with ADP-AlF4

Mitogen-activated protein kinase 14Dual specificity mitogen-activated protein kinase kinase 6Adenosine 5'-Diphosphate Tetrafluoroaluminate experimental SAS data
Mitogen-activated protein kinase 14 Dual specificity mitogen-activated protein kinase kinase 6 Adenosine 5'-Diphosphate Tetrafluoroaluminate Kratky plot
Sample: Mitogen-activated protein kinase 14 monomer, 40 kDa Homo sapiens protein
Dual specificity mitogen-activated protein kinase kinase 6 monomer, 42 kDa synthetic construct protein
Adenosine 5'-Diphosphate Tetrafluoroaluminate monomer, 1 kDa
Buffer: 50 mM HEPES, 200 mM NaCl, 10 mM MgCl2, 5 % glycerol, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 May 15
Architecture of the MKK6-p38α complex defines the basis of MAPK specificity and activation Science 381(6663):1217-1225 (2023)
Juyoux P, Galdadas I, Gobbo D, von Velsen J, Pelosse M, Tully M, Vadas O, Gervasio F, Pellegrini E, Bowler M
RgGuinier 3.2 nm
Dmax 12.1 nm
VolumePorod 113 nm3

SASDRD6 – Dual specificity mitogen-activated protein kinase kinase 6 (MKK6GRA) bound to mitogen-activated protein kinase 14 (p38α) with AMP-PCP

Mitogen-activated protein kinase 14Dual specificity mitogen-activated protein kinase kinase 65′‐adenylyl methylenediphosphonate experimental SAS data
Mitogen-activated protein kinase 14 Dual specificity mitogen-activated protein kinase kinase 6 5′‐adenylyl methylenediphosphonate Kratky plot
Sample: Mitogen-activated protein kinase 14 monomer, 40 kDa Homo sapiens protein
Dual specificity mitogen-activated protein kinase kinase 6 monomer, 42 kDa synthetic construct protein
5′‐adenylyl methylenediphosphonate monomer, 1 kDa
Buffer: 50 mM HEPES, 200 mM NaCl, 10 mM MgCl2, 5 % glycerol, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 May 15
Architecture of the MKK6-p38α complex defines the basis of MAPK specificity and activation Science 381(6663):1217-1225 (2023)
Juyoux P, Galdadas I, Gobbo D, von Velsen J, Pelosse M, Tully M, Vadas O, Gervasio F, Pellegrini E, Bowler M
RgGuinier 4.2 nm
Dmax 14.0 nm
VolumePorod 116 nm3

SASDS22 – Sulfite reductase flavoprotein crosslinked octamer

Sulfite reductase [NADPH] flavoprotein alpha-component experimental SAS data
SASREF model
Sample: Sulfite reductase [NADPH] flavoprotein alpha-component octamer, 530 kDa Escherichia coli (strain … protein
Buffer: 50 mM potassium phosphate, 100 mM NaCl, 1 mM EDTA, pH: 7.8
Experiment: SANS data collected at EQ-SANS, Spallation Neutron Source on 2022 Jun 24
Domain crossover in the reductase subunit of NADPH-dependent assimilatory sulfite reductase. J Struct Biol 215(4):108028 (2023)
Walia N, Murray DT, Garg Y, He H, Weiss KL, Nagy G, Elizabeth Stroupe M
RgGuinier 8.4 nm
Dmax 23.0 nm
VolumePorod 767 nm3

SASDS32 – Reconstituted sulfite reductase crosslinked dodecamer with partially deuterated hemoprotein in 86% D2O

Sulfite reductase [NADPH] hemoprotein beta-componentSulfite reductase [NADPH] flavoprotein alpha-component (E121C, C162T, C552S, N556C) experimental SAS data
Sample: Sulfite reductase [NADPH] hemoprotein beta-component tetramer, 256 kDa Escherichia coli (strain … protein
Sulfite reductase [NADPH] flavoprotein alpha-component (E121C, C162T, C552S, N556C) octamer, 566 kDa Escherichia coli (strain … protein
Buffer: 50 mM potassium phosphate, 100 mM NaCl, 1 mM EDTA, pH: 7.8
Experiment: SANS data collected at EQ-SANS, Spallation Neutron Source on 2023 Apr 24
Domain crossover in the reductase subunit of NADPH-dependent assimilatory sulfite reductase. J Struct Biol 215(4):108028 (2023)
Walia N, Murray DT, Garg Y, He H, Weiss KL, Nagy G, Elizabeth Stroupe M
RgGuinier 7.5 nm
Dmax 22.6 nm
VolumePorod 823 nm3

SASDS42 – Reconstituted sulfite reductase crosslinked dodecamer with partially deuterated hemoprotein in 41% D2O

Sulfite reductase [NADPH] hemoprotein beta-componentSulfite reductase [NADPH] flavoprotein alpha-component (E121C, C162T, C552S, N556C) experimental SAS data
Sample: Sulfite reductase [NADPH] hemoprotein beta-component tetramer, 256 kDa Escherichia coli (strain … protein
Sulfite reductase [NADPH] flavoprotein alpha-component (E121C, C162T, C552S, N556C) octamer, 566 kDa Escherichia coli (strain … protein
Buffer: 50 mM potassium phosphate, 100 mM NaCl, 1 mM EDTA, pH: 7.8
Experiment: SANS data collected at EQ-SANS, Spallation Neutron Source on 2022 Aug 24
Domain crossover in the reductase subunit of NADPH-dependent assimilatory sulfite reductase. J Struct Biol 215(4):108028 (2023)
Walia N, Murray DT, Garg Y, He H, Weiss KL, Nagy G, Elizabeth Stroupe M
RgGuinier 11.1 nm
Dmax 32.4 nm
VolumePorod 138 nm3

SASDS52 – Sulfite reductase crosslinked dodecamer

Sulfite reductase [NADPH] hemoprotein beta-componentSulfite reductase [NADPH] flavoprotein alpha-component (E121C, C162T, C552S, N556C) experimental SAS data
Sample: Sulfite reductase [NADPH] hemoprotein beta-component tetramer, 256 kDa Escherichia coli (strain … protein
Sulfite reductase [NADPH] flavoprotein alpha-component (E121C, C162T, C552S, N556C) octamer, 566 kDa Escherichia coli (strain … protein
Buffer: 50 mM potassium phosphate, 100 mM NaCl, 1 mM EDTA, pH: 7.8
Experiment: SANS data collected at EQ-SANS, Spallation Neutron Source on 2022 Jul 15
Domain crossover in the reductase subunit of NADPH-dependent assimilatory sulfite reductase. J Struct Biol 215(4):108028 (2023)
Walia N, Murray DT, Garg Y, He H, Weiss KL, Nagy G, Elizabeth Stroupe M
RgGuinier 10.3 nm
Dmax 31.0 nm
VolumePorod 1170 nm3

SASDS62 – Sulfite reductase flavoprotein truncated linker (ΔAAPSQS) octamer

Sulfite reductase [NADPH] flavoprotein alpha-component (Δ212-217) experimental SAS data
Sulfite reductase [NADPH] flavoprotein alpha-component (Δ212-217) Kratky plot
Sample: Sulfite reductase [NADPH] flavoprotein alpha-component (Δ212-217) octamer, 526 kDa Escherichia coli (strain … protein
Buffer: 50 mM potassium phosphate, 100 mM NaCl, 1 mM EDTA, pH: 7.8
Experiment: SANS data collected at EQ-SANS, Spallation Neutron Source on 2022 Jun 25
Domain crossover in the reductase subunit of NADPH-dependent assimilatory sulfite reductase. J Struct Biol 215(4):108028 (2023)
Walia N, Murray DT, Garg Y, He H, Weiss KL, Nagy G, Elizabeth Stroupe M
RgGuinier 13.7 nm
Dmax 25.5 nm
VolumePorod 961 nm3

SASDS72 – Sulfite reductase truncated linker (ΔAAPSQS) dodecamer

Sulfite reductase [NADPH] hemoprotein beta-componentSulfite reductase [NADPH] flavoprotein alpha-component (Δ212-217) experimental SAS data
Sulfite reductase [NADPH] hemoprotein beta-component Sulfite reductase [NADPH] flavoprotein alpha-component (Δ212-217) Kratky plot
Sample: Sulfite reductase [NADPH] hemoprotein beta-component tetramer, 256 kDa Escherichia coli (strain … protein
Sulfite reductase [NADPH] flavoprotein alpha-component (Δ212-217) octamer, 528 kDa Escherichia coli (strain … protein
Buffer: 50 mM potassium phosphate, 100 mM NaCl, 1 mM EDTA, pH: 7.8
Experiment: SANS data collected at EQ-SANS, Spallation Neutron Source on 2022 Jun 26
Domain crossover in the reductase subunit of NADPH-dependent assimilatory sulfite reductase. J Struct Biol 215(4):108028 (2023)
Walia N, Murray DT, Garg Y, He H, Weiss KL, Nagy G, Elizabeth Stroupe M
RgGuinier 10.8 nm
Dmax 30.3 nm
VolumePorod 1260 nm3

SASDS82 – Reconstituted sulfite reductase crosslinked dodecamer with partially deuterated hemoprotein in 0% D2O

Sulfite reductase [NADPH] flavoprotein alpha-component (E121C, C162T, C552S, N556C)Sulfite reductase [NADPH] hemoprotein beta-component experimental SAS data
Sulfite reductase [NADPH] flavoprotein alpha-component (E121C, C162T, C552S, N556C) Sulfite reductase [NADPH] hemoprotein beta-component Kratky plot
Sample: Sulfite reductase [NADPH] flavoprotein alpha-component (E121C, C162T, C552S, N556C) octamer, 566 kDa Escherichia coli (strain … protein
Sulfite reductase [NADPH] hemoprotein beta-component tetramer, 256 kDa Escherichia coli (strain … protein
Buffer: 50 mM potassium phosphate, 100 mM NaCl, 1 mM EDTA, pH: 7.8
Experiment: SANS data collected at EQ-SANS, Spallation Neutron Source on 2022 Jul 2
Domain crossover in the reductase subunit of NADPH-dependent assimilatory sulfite reductase. J Struct Biol 215(4):108028 (2023)
Walia N, Murray DT, Garg Y, He H, Weiss KL, Nagy G, Elizabeth Stroupe M
RgGuinier 9.5 nm
Dmax 28.3 nm
VolumePorod 1010 nm3

SASDS92 – Reconstituted sulfite reductase crosslinked dodecamer with partially deuterated hemoprotein in 100% D2O

Sulfite reductase [NADPH] hemoprotein beta-componentSulfite reductase [NADPH] flavoprotein alpha-component (E121C, C162T, C552S, N556C) experimental SAS data
Sulfite reductase [NADPH] hemoprotein beta-component Sulfite reductase [NADPH] flavoprotein alpha-component (E121C, C162T, C552S, N556C) Kratky plot
Sample: Sulfite reductase [NADPH] hemoprotein beta-component tetramer, 256 kDa Escherichia coli (strain … protein
Sulfite reductase [NADPH] flavoprotein alpha-component (E121C, C162T, C552S, N556C) octamer, 566 kDa Escherichia coli (strain … protein
Buffer: 50 mM potassium phosphate, 100 mM NaCl, 1 mM EDTA, pH: 7.8
Experiment: SANS data collected at EQ-SANS, Spallation Neutron Source on 2022 Jun 27
Domain crossover in the reductase subunit of NADPH-dependent assimilatory sulfite reductase. J Struct Biol 215(4):108028 (2023)
Walia N, Murray DT, Garg Y, He H, Weiss KL, Nagy G, Elizabeth Stroupe M
RgGuinier 8.2 nm
Dmax 25.1 nm
VolumePorod 839 nm3