Small Angle Scattering Biological Data Bank SASBDB

Browse by MACROMOLECULE type: protein

SASDE52 – Ribonuclease E from Escherichia coli

Endoribonuclease E experimental SAS data

Sample:	Endoribonuclease E tetramer, 247 kDa Escherichia coli protein
Buffer:	10 mM DTT, 10 mM MgCl2, 0.5 M NaCl, 20 mM Tris, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2017 Feb 11

A structural and biochemical comparison of Ribonuclease E homologues from pathogenic bacteria highlights species-specific properties. Sci Rep 9(1):7952 (2019)
Mardle CE, Shakespeare TJ, Butt LE, Goddard LR, Gowers DM, Atkins HS, Vincent HA, Callaghan AJ

R_g^Guinier	5.0	nm
D_max	16.1	nm
Volume^Porod	468	nm³

SASDE62 – Ribonuclease E from Yersinia pestis

Sample:	Endoribonuclease E tetramer, 248 kDa Yersinia pestis protein
Buffer:	10 mM DTT, 10 mM MgCl2, 0.5 M NaCl, 20 mM Tris, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2017 Feb 11

R_g^Guinier	5.1	nm
D_max	16.4	nm
Volume^Porod	470	nm³

SASDE72 – Ribonuclease E from Francisella tularensis

Sample:	Endoribonuclease E tetramer, 256 kDa Francisella tularensis protein
Buffer:	10 mM DTT, 10 mM MgCl2, 0.5 M NaCl, 20 mM Tris, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2017 Feb 11

R_g^Guinier	5.1	nm
D_max	17.2	nm
Volume^Porod	491	nm³

SASDE82 – Ribonuclease E from Burkholderia pseudomallei

Sample:	Endoribonuclease E tetramer, 250 kDa Burkholderia pseudomallei protein
Buffer:	10 mM DTT, 10 mM MgCl2, 0.5 M NaCl, 20 mM Tris, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2017 Feb 11

R_g^Guinier	4.8	nm
D_max	14.9	nm
Volume^Porod	437	nm³

SASDE92 – Ribonuclease E from Acinetobacter baumannii

Sample:	Endoribonuclease E tetramer, 254 kDa Acinetobacter baumannii protein
Buffer:	10 mM DTT, 10 mM MgCl2, 0.5 M NaCl, 20 mM Tris, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2017 Feb 11

R_g^Guinier	5.2	nm
D_max	18.3	nm
Volume^Porod	508	nm³

SASDFK8 – GON7, the fifth subunit of human KEOPS

EKC/KEOPS complex subunit GON7 experimental SAS data

EKC/KEOPS complex subunit GON7 Kratky plot

Sample:	EKC/KEOPS complex subunit GON7 monomer, 13 kDa Homo sapiens protein
Buffer:	20 mM MES, 200 mM NaCl, 5 mM β-mercaptoethanol, pH: 6.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2017 Mar 26

Defects in t⁶A tRNA modification due to GON7 and YRDC mutations lead to Galloway-Mowat syndrome. Nat Commun 10(1):3967 (2019)
Arrondel C, Missoury S, Snoek R, Patat J, Menara G, Collinet B, Liger D, Durand D, Gribouval O, Boyer O, Buscara L, Martin G, Machuca E, Nevo F, Lescop E, Braun DA, Boschat AC, Sanquer S, Guerrera IC, Revy P, Parisot M, Masson C, Boddaert N, Charbit M, Decramer S, Novo R, Macher MA, Ranchin B, Bacchetta J, Laurent A, Collardeau-Frachon S, van Eerde AM, Hildebrandt F, Magen D, Antignac C, van Tilbeurgh H, Mollet G

R_g^Guinier	3.1	nm
D_max	12.5	nm
Volume^Porod	46	nm³

SASDFL8 – GON7, the fifth subunit of human KEOPS, bound to LAGE3-OSGEP (EKC/KEOPS complex subunit:Probable tRNA N6-adenosine threonylcarbamoyltransferase)

EKC/KEOPS complex subunit GON7EKC/KEOPS complex subunit LAGE3Probable tRNA N6-adenosine threonylcarbamoyltransferase experimental SAS data

Sample:	EKC/KEOPS complex subunit GON7 monomer, 12 kDa Homo sapiens protein EKC/KEOPS complex subunit LAGE3 monomer, 15 kDa Homo sapiens protein Probable tRNA N6-adenosine threonylcarbamoyltransferase monomer, 36 kDa Homo sapiens protein
Buffer:	20 mM MES, 200 mM NaCl, 5 mM β-mercaptoethanol, pH: 6.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2017 May 12

R_g^Guinier	3.1	nm
D_max	11.5	nm
Volume^Porod	91	nm³

SASDFM8 – GON7, the fifth subunit of human KEOPS, bound to the EKC/KEOPS complex subunit, LAGE3

EKC/KEOPS complex subunit GON7EKC/KEOPS complex subunit LAGE3 experimental SAS data

EKC/KEOPS complex subunit GON7 EKC/KEOPS complex subunit LAGE3 Kratky plot

Sample:	EKC/KEOPS complex subunit GON7 dimer, 25 kDa Homo sapiens protein EKC/KEOPS complex subunit LAGE3 dimer, 33 kDa Homo sapiens protein
Buffer:	20 mM MES, 200 mM NaCl, 5 mM β-mercaptoethanol, pH: 6.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2017 Mar 19

R_g^Guinier	3.6	nm
D_max	15.5	nm
Volume^Porod	126	nm³

SASDD75 – 'TG'-interacting factor homeodomain in solution (Homeobox protein TGIF1)

Homeobox protein TGIF1 experimental SAS data

'TG'-interacting factor homeodomain in solution (Homeobox protein TGIF1) Rg histogram

Sample:	Homeobox protein TGIF1 dimer, 23 kDa Homo sapiens protein
Buffer:	20 mM Tris, 150 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Oct 18

'TG' interacting factor
Ewelina Guca

R_g^Guinier	2.7	nm
D_max	9.2	nm
Volume^Porod	42	nm³

SASDE99 – Calredoxin with EGTA

Calredoxin, Redox protein from Chlamydomonas reinhardtii experimental SAS data

Calredoxin, Redox protein from Chlamydomonas reinhardtii Kratky plot

Sample:	Calredoxin, Redox protein from Chlamydomonas reinhardtii monomer, 40 kDa Chlamydomonas reinhardtii protein
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM DTT, 5 mM EGTA, pH: 8
Experiment:	SAXS data collected at Rigaku BioSAXS-1000, Structural Biology Laboratory, Graduate School of Medical Life Science, Yokohama City University on 2015 Nov 17

Calcium sensing via EF-hand 4 enables thioredoxin activity in the sensor-responder protein calredoxin in the green alga Chlamydomonas reinhardtii. J Biol Chem (2019)
Charoenwattanasatien R, Zinzius K, Scholz M, Wicke S, Tanaka H, Brandenburg JS, Marchetti GM, Ikegami T, Matsumoto T, Oda T, Sato M, Hippler M, Kurisu G

R_g^Guinier	2.5	nm
D_max	8.7	nm
Volume^Porod	60	nm³

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