Browse by MACROMOLECULE type: protein

SASDDA8 – Apolipoprotein E4

Apolipoprotein E4 experimental SAS data
Apolipoprotein E4 Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Nov 29
The molecular basis for Apolipoprotein E4 as the major risk factor for late onset Alzheimer's disease. J Mol Biol (2019)
Raulin AC, Kraft L, Al-Hilaly YK, Xue WF, McGeehan JE, Atack JR, Serpell L
RgGuinier 5.8 nm
Dmax 19.6 nm
VolumePorod 430 nm3

SASDC45 – Alpha domain of autotransporter protein UpaB from UPEC

Alpha domain of autotransporter protein UpaB experimental SAS data
CORAL model
Sample: Alpha domain of autotransporter protein UpaB monomer, 48 kDa E. Coli CFT073 protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2015 May 1
Unique structural features of a bacterial autotransporter adhesin suggest mechanisms for interaction with host macromolecules. Nat Commun 10(1):1967 (2019)
Paxman JJ, Lo AW, Sullivan MJ, Panjikar S, Kuiper M, Whitten AE, Wang G, Luan CH, Moriel DG, Tan L, Peters KM, Phan MD, Gee CL, Ulett GC, Schembri MA, Heras B
RgGuinier 2.9 nm
Dmax 10.5 nm
VolumePorod 66 nm3

SASDDH9 – Chloroplastic phosphoribulokinase (collected using SEC-SAXS)

Phosphoribulokinase, chloroplastic experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Phosphoribulokinase, chloroplastic dimer, 78 kDa Chlamydomonas reinhardtii protein
Buffer: Tris-HCl 50 mM 150 mM KCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Feb 15
Arabidopsis and Chlamydomonas phosphoribulokinase crystal structures complete the redox structural proteome of the Calvin-Benson cycle. Proc Natl Acad Sci U S A 116(16):8048-8053 (2019)
Gurrieri L, Del Giudice A, Demitri N, Falini G, Pavel NV, Zaffagnini M, Polentarutti M, Crozet P, Marchand CH, Henri J, Trost P, Lemaire SD, Sparla F, Fermani S
RgGuinier 3.4 nm
Dmax 11.3 nm
VolumePorod 115 nm3

SASDFR4 – HP2042 form from Helicobacter pylori, N-terminal domain of syntaxin-1A from Rattus norvegicus, Trp repressor from Escherichia coli (Domain A (PDB:3MLI)-Linker (PDB:1BR0)-Domain B (PDB:1WRT))

HP2042 form from Helicobacter pylori, N-terminal domain of syntaxin-1A from Rattus norvegicus, Trp repressor from Escherichia coli experimental SAS data
CORAL model
Sample: HP2042 form from Helicobacter pylori, N-terminal domain of syntaxin-1A from Rattus norvegicus, Trp repressor from Escherichia coli tetramer, 150 kDa Helicobacter pylori, Rattus … protein
Buffer: 20 mM Tris-HCl 150 mM NaCl, pH: 8
Experiment: SAXS data collected at Rigaku Nano-Viewer, Nara Institute of Science and Technology on 2016 Dec 8
Construction of a Quadrangular Tetramer and a Cage-Like Hexamer from Three-Helix Bundle-Linked Fusion Proteins. ACS Synth Biol (2019)
Miyamoto T, Hayashi Y, Yoshida K, Watanabe H, Uchihashi T, Yonezawa K, Shimizu N, Kamikubo H, Hirota S
RgGuinier 5.1 nm
Dmax 20.0 nm
VolumePorod 379 nm3

SASDFS4 – HP0242 from Helicobacter pylori, N-terminal domain of syntaxin-1A from Rattus norvegicus, de novo designed coiled-coil trimer domain (Domain A (PDB:3MLI)-Linker (PDB:1BR0)-Domain B (PDB:4DZL))

HP0242 from Helicobacter pylori, N-terminal domain of syntaxin-1A from Rattus norvegicus, de novo designed coiled-coil trimer domain experimental SAS data
CORAL model
Sample: HP0242 from Helicobacter pylori, N-terminal domain of syntaxin-1A from Rattus norvegicus, de novo designed coiled-coil trimer domain hexamer, 179 kDa Helicobacter pylori, Rattus … protein
Buffer: 20 mM Tris-HCl 150 mM NaCl, pH: 8
Experiment: SAXS data collected at Rigaku Nano-Viewer, Nara Institute of Science and Technology on 2017 Mar 29
Construction of a Quadrangular Tetramer and a Cage-Like Hexamer from Three-Helix Bundle-Linked Fusion Proteins. ACS Synth Biol (2019)
Miyamoto T, Hayashi Y, Yoshida K, Watanabe H, Uchihashi T, Yonezawa K, Shimizu N, Kamikubo H, Hirota S
RgGuinier 6.5 nm
Dmax 24.0 nm
VolumePorod 642 nm3

SASDFU3 – Lysine-specific Demethylase (LSD2)

Lysyne-specific Demethylase LSD2 experimental SAS data
Lysyne-specific Demethylase LSD2 Kratky plot
Sample: Lysyne-specific Demethylase LSD2 monomer, 89 kDa Homo sapiens protein
Buffer: 15 mM HEPES, 200 mM NaCl, pH: 7.3
Experiment: SAXS data collected at BM29, ESRF on 2017 Dec 11
A Tail-Based Mechanism Drives Nucleosome Demethylation by the LSD2/NPAC Multimeric Complex. Cell Rep 27(2):387-399.e7 (2019)
Marabelli C, Marrocco B, Pilotto S, Chittori S, Picaud S, Marchese S, Ciossani G, Forneris F, Filippakopoulos P, Schoehn G, Rhodes D, Subramaniam S, Mattevi A
RgGuinier 3.4 nm
Dmax 10.6 nm
VolumePorod 124 nm3

SASDFV3 – Cytokine-like nuclear factor dehydrogenase domain, NPAC DH (NPAC delta-261)

NPAC dehydrogenase domain experimental SAS data
NPAC dehydrogenase domain Kratky plot
Sample: NPAC dehydrogenase domain tetramer, 125 kDa Homo sapiens protein
Buffer: 15 mM HEPES, 200 mM NaCl, pH: 7.3
Experiment: SAXS data collected at BM29, ESRF on 2018 Jan 26
A Tail-Based Mechanism Drives Nucleosome Demethylation by the LSD2/NPAC Multimeric Complex. Cell Rep 27(2):387-399.e7 (2019)
Marabelli C, Marrocco B, Pilotto S, Chittori S, Picaud S, Marchese S, Ciossani G, Forneris F, Filippakopoulos P, Schoehn G, Rhodes D, Subramaniam S, Mattevi A
RgGuinier 3.5 nm
Dmax 11.0 nm
VolumePorod 167 nm3

SASDFW3 – Cytokine-like nuclear factor dehydrogenase domain, NPAC DH, plus native linker (NPAC delta-205)

NPAC linker+DH (delta-205) experimental SAS data
NPAC linker+DH (delta-205) Kratky plot
Sample: NPAC linker+DH (delta-205) tetramer, 150 kDa Homo sapiens protein
Buffer: 15 mM HEPES, 200 mM NaCl, pH: 7.3
Experiment: SAXS data collected at BM29, ESRF on 2018 Jan 26
A Tail-Based Mechanism Drives Nucleosome Demethylation by the LSD2/NPAC Multimeric Complex. Cell Rep 27(2):387-399.e7 (2019)
Marabelli C, Marrocco B, Pilotto S, Chittori S, Picaud S, Marchese S, Ciossani G, Forneris F, Filippakopoulos P, Schoehn G, Rhodes D, Subramaniam S, Mattevi A
RgGuinier 4.0 nm
Dmax 11.4 nm
VolumePorod 240 nm3

SASDE36 – Human ATP-citrate synthase (ACLY) in HBS

ATP-citrate synthase experimental SAS data
MULTIFOXS model
Sample: ATP-citrate synthase tetramer, 458 kDa Homo sapiens protein
Buffer: 20mM HEPES, 150mM NaCl, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 May 6
Structure of ATP citrate lyase and the origin of citrate synthase in the Krebs cycle. Nature 568(7753):571-575 (2019)
Verschueren KHG, Blanchet C, Felix J, Dansercoer A, De Vos D, Bloch Y, Van Beeumen J, Svergun D, Gutsche I, Savvides SN, Verstraete K
RgGuinier 6.0 nm
Dmax 17.5 nm
VolumePorod 738 nm3

SASDE46 – Human ATP-citrate synthers (ACLY) in HBS + Citrate

ATP-citrate synthase experimental SAS data
ATP-citrate synthase Kratky plot
Sample: ATP-citrate synthase tetramer, 458 kDa Homo sapiens protein
Buffer: 20mM HEPES, 150mM NaCl, 50mM Tris, 20mM citrate, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 May 6
Structure of ATP citrate lyase and the origin of citrate synthase in the Krebs cycle. Nature 568(7753):571-575 (2019)
Verschueren KHG, Blanchet C, Felix J, Dansercoer A, De Vos D, Bloch Y, Van Beeumen J, Svergun D, Gutsche I, Savvides SN, Verstraete K
RgGuinier 6.1 nm
Dmax 17.5 nm
VolumePorod 747 nm3