Small Angle Scattering Biological Data Bank SASBDB

Browse by MACROMOLECULE type: protein

SASDB32 – Human Filamin A Ig-like domains 16-17 truncation (1782-1956)

Human Filamin A Ig-like domains 16-17 experimental SAS data

Sample:	Human Filamin A Ig-like domains 16-17 monomer, 19 kDa Homo sapiens protein
Buffer:	20 mM Tris 50 mM NaCl 10 mM DTT, pH:
Experiment:	SAXS data collected at BM29, ESRF on 2013 Sep 30

Skeletal Dysplasia Mutations Effect on Human Filamins' Structure and Mechanosensing. Sci Rep 7(1):4218 (2017)
Seppälä J, Bernardi RC, Haataja TJK, Hellman M, Pentikäinen OT, Schulten K, Permi P, Ylänne J, Pentikäinen U

R_g^Guinier	1.8	nm
D_max	6.2	nm
Volume^Porod	31	nm³

SASDB42 – Human Filamin A Ig-like domains 16-17* truncation (1782-1956) L1788R patient mutation

Sample:	Human Filamin A Ig-like domains 16-17* monomer, 19 kDa Homo sapiens protein
Buffer:	20 mM Tris 50 mM NaCl 10 mM DTT, pH:
Experiment:	SAXS data collected at BM29, ESRF on 2013 Sep 30

R_g^Guinier	2.4	nm
D_max	8.3	nm
Volume^Porod	37	nm³

SASDC63 – Fowlpox Virus FPV039 antiapoptotic Bcl-2 viral protein in complex with BaK BH3 peptide (FPV039:BAK)

Bcl-2-like protein FPV039Uncharacterized protein (BAK1) experimental SAS data

Bcl-2-like protein FPV039 Uncharacterized protein (BAK1) Kratky plot

Sample:	Bcl-2-like protein FPV039 monomer, 17 kDa Fowlpox virus protein Uncharacterized protein (BAK1) monomer, 3 kDa Gallus gallus protein
Buffer:	20 mM trisodium citrate pH, 200 mM NaCl, pH: 6
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2015 Oct 2

Structural basis of apoptosis inhibition by the fowlpox virus protein FPV039. J Biol Chem 292(22):9010-9021 (2017)
Anasir MI, Caria S, Skinner MA, Kvansakul M

R_g^Guinier

2.0

SASDBM6 – Nucleolysin TIA-1 isoform p40

Nucleolysin TIA-1 isoform p40 experimental SAS data

Nucleolysin TIA-1 isoform p40 Kratky plot

Sample:	Nucleolysin TIA-1 isoform p40 monomer, 21 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 3% v/v glycerol, pH: 7
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2015 Jul 2

TIA-1 RRM23 binding and recognition of target oligonucleotides. Nucleic Acids Res 45(8):4944-4957 (2017)
Waris S, García-Mauriño SM, Sivakumaran A, Beckham SA, Loughlin FE, Gorospe M, Díaz-Moreno I, Wilce MCJ, Wilce JA

R_g^Guinier	2.3	nm
D_max	8.8	nm
Volume^Porod	26	nm³

SASDB59 – Recombinant monomeric human Properdin

Human recombinant Properdin TSR 0-3Human recombinant Properdin TSR 4-6 experimental SAS data

Sample:	Human recombinant Properdin TSR 0-3 monomer, 25 kDa Homo sapiens protein Human recombinant Properdin TSR 4-6 monomer, 24 kDa Homo sapiens protein
Buffer:	10 mM HEPES 150 mM NaCl, pH: 7.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2015 Aug 16

Functional and structural insight into properdin control of complement alternative pathway amplification. EMBO J 36(8):1084-1099 (2017)
Pedersen DV, Roumenina L, Jensen RK, Gadeberg TA, Marinozzi C, Picard C, Rybkine T, Thiel S, Sørensen UB, Stover C, Fremeaux-Bacchi V, Andersen GR

R_g^Guinier	5.1	nm
D_max	18.0	nm

SASDB69 – E244K monomeric human Properdin

E244K Human Properdin experimental SAS data

Sample:	E244K Human Properdin monomer, 49 kDa Homo sapiens protein
Buffer:	10 mM HEPES 150 mM NaCl, pH: 7.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Jul 27

R_g^Guinier	4.1	nm
D_max	15.0	nm

SASDBE7 – ESX-5 type VII secretion system protein EccC5

ESX-5 type VII secretion system protein EccC5 experimental SAS data

ESX-5 type VII secretion system protein EccC5 Rg histogram

Sample:	ESX-5 type VII secretion system protein EccC5 monomer, 142 kDa Mycobacterium xenopi RIVM700367 protein
Buffer:	20 mM Tris 200 mM NaCl 5%(v/v) Glycerol, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Jul 15

Structure of the mycobacterial ESX-5 type VII secretion system membrane complex by single-particle analysis. Nat Microbiol 2:17047 (2017)
Beckham KS, Ciccarelli L, Bunduc CM, Mertens HD, Ummels R, Lugmayr W, Mayr J, Rettel M, Savitski MM, Svergun DI, Bitter W, Wilmanns M, Marlovits TC, Parret AH, Houben EN

R_g^Guinier	6.2	nm
D_max	23.0	nm
Volume^Porod	255	nm³

SASDBS6 – Apo form of full length ObgE from E.coli (ObgE_FL)

Apo form of full length ObgE from E.coli (ObgE_FL) Rg histogram

Sample:	GTPase ObgE/CgtA monomer, 44 kDa Escherichia coli protein
Buffer:	20 mM Hepes , 300 mM NaCl, 250 mM imidazole, 5 mM MgCl2, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2015 Jun 17

Structural and biochemical analysis of Escherichia coli ObgE, a central regulator of bacterial persistence. J Biol Chem 292(14):5871-5883 (2017)
Gkekas S, Singh RK, Shkumatov AV, Messens J, Fauvart M, Verstraeten N, Michiels J, Versées W

R_g^Guinier	3.7	nm
D_max	18.1	nm
Volume^Porod	102	nm³

SASDBT6 – GppNHp bound form of full length ObgE from E.coli (ObgE_FL with GppNHp)

Sample:	GTPase ObgE/CgtA monomer, 44 kDa Escherichia coli protein
Buffer:	20 mM Hepes, 300 mM NaCl, 250 mM imidazole, 5 mM MgCl2, 2 mM DTT, 400 µM GppNHp, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2015 Jun 17

R_g^Guinier	3.7	nm
D_max	14.5	nm
Volume^Porod	90	nm³

SASDBU6 – Apo form of the C-terminal deletion mutant of ObgE from E.coli (ObgE_340)

Apo form of the C-terminal deletion mutant of ObgE from E.coli (ObgE_340) Rg histogram

Sample:	GTPase ObgE/CgtA monomer, 39 kDa Escherichia coli protein
Buffer:	20 mM Hepes , 300 mM NaCl, 250 mM imidazole, 5 mM MgCl2, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2015 Mar 12

R_g^Guinier	3.1	nm
D_max	11.6	nm
Volume^Porod	70	nm³

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