Browse by MACROMOLECULE type: protein

SASDBF3 – Leishmania braziliensis heat shock protein 90 (Hsp90) N and M domains.

Leishmania braziliensis heat shock protein 90 (Hsp90) N and M domains experimental SAS data
DAMMIN model
Sample: Leishmania braziliensis heat shock protein 90 (Hsp90) N and M domains monomer, 62 kDa Leishmania braziliensis protein
Buffer: 25 Tris mM 100 mM NaCl 1 mM 2-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at SAXS2 Beamline, Brazilian Synchrotron Light Laboratory on 2011 Sep 1
Insights on the structural dynamics of Leishmania braziliensis Hsp90 molecular chaperone by small angle X-ray scattering. Int J Biol Macromol 97:503-512 (2017)
Seraphim TV, Silva KP, Dores-Silva PR, Barbosa LR, Borges JC
RgGuinier 3.2 nm
Dmax 13.0 nm
VolumePorod 89 nm3

SASDBU7 – DNA binding domain (DBD) of chromo domain-containing protein 1 (Chd1: 1009-1274)

chromodomain helicase DNA binding domain experimental SAS data
GASBOR model
Sample: Chromodomain helicase DNA binding domain monomer, 31 kDa Saccharomyces cerevisiae protein
Buffer: 50mM Hepes 150mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Nov 20
Structural reorganization of the chromatin remodeling enzyme Chd1 upon engagement with nucleosomes. Elife 6 (2017)
Sundaramoorthy R, Hughes AL, Singh V, Wiechens N, Ryan DP, El-Mkami H, Petoukhov M, Svergun DI, Treutlein B, Quack S, Fischer M, Michaelis J, Böttcher B, Norman DG, Owen-Hughes T
RgGuinier 2.6 nm
Dmax 8.3 nm

SASDBV7 – Chromo-ATPase-DBD domains of chromo domain-containing protein 1 (Chd1: 133-1305)

chromodomain helicase DNA binding domain experimental SAS data
GASBOR model
Sample: Chromodomain helicase DNA binding domain monomer, 135 kDa Saccharomyces cerevisiae protein
Buffer: 50mM Hepes 150mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2008 Nov 30
Structural reorganization of the chromatin remodeling enzyme Chd1 upon engagement with nucleosomes. Elife 6 (2017)
Sundaramoorthy R, Hughes AL, Singh V, Wiechens N, Ryan DP, El-Mkami H, Petoukhov M, Svergun DI, Treutlein B, Quack S, Fischer M, Michaelis J, Böttcher B, Norman DG, Owen-Hughes T
RgGuinier 4.2 nm
Dmax 15.4 nm
VolumePorod 280 nm3

SASDBW7 – N-terminal and chromo-ATPase-DBD domains of chromo domain-containing protein 1 (Chd1: 1-1305)

chromodomain helicase DNA binding domain experimental SAS data
GASBOR model
Sample: Chromodomain helicase DNA binding domain monomer, 150 kDa Saccharomyces cerevisiae protein
Buffer: 50mM Hepes 150mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2008 Nov 30
Structural reorganization of the chromatin remodeling enzyme Chd1 upon engagement with nucleosomes. Elife 6 (2017)
Sundaramoorthy R, Hughes AL, Singh V, Wiechens N, Ryan DP, El-Mkami H, Petoukhov M, Svergun DI, Treutlein B, Quack S, Fischer M, Michaelis J, Böttcher B, Norman DG, Owen-Hughes T
RgGuinier 4.9 nm
Dmax 16.0 nm
VolumePorod 340 nm3

SASDBX7 – Chromo-ATPase domains of chromo domain-containing protein 1 (Chd1: 133-1010)

chromodomain helicase DNA binding domain experimental SAS data
GASBOR model
Sample: Chromodomain helicase DNA binding domain monomer, 102 kDa Saccharomyces cerevisiae protein
Buffer: 50mM Hepes 150mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2008 Nov 30
Structural reorganization of the chromatin remodeling enzyme Chd1 upon engagement with nucleosomes. Elife 6 (2017)
Sundaramoorthy R, Hughes AL, Singh V, Wiechens N, Ryan DP, El-Mkami H, Petoukhov M, Svergun DI, Treutlein B, Quack S, Fischer M, Michaelis J, Böttcher B, Norman DG, Owen-Hughes T
RgGuinier 4.1 nm
Dmax 16.1 nm
VolumePorod 190 nm3

SASDBY7 – N-terminal and chromo-ATPase domains of chromo domain-containing protein 1 (Chd1: 1-1010)

chromodomain helicase DNA binding domain experimental SAS data
GASBOR model
Sample: Chromodomain helicase DNA binding domain monomer, 117 kDa Saccharomyces cerevisiae protein
Buffer: 50mM Hepes 150mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2008 Nov 30
Structural reorganization of the chromatin remodeling enzyme Chd1 upon engagement with nucleosomes. Elife 6 (2017)
Sundaramoorthy R, Hughes AL, Singh V, Wiechens N, Ryan DP, El-Mkami H, Petoukhov M, Svergun DI, Treutlein B, Quack S, Fischer M, Michaelis J, Böttcher B, Norman DG, Owen-Hughes T
RgGuinier 4.5 nm
Dmax 12.0 nm
VolumePorod 228 nm3

SASDBG6 – Ribosome biogenesis protein 15 (Nop15)

Ribosome biogenesis protein 15 experimental SAS data
EOM/RANCH model
Sample: Ribosome biogenesis protein 15 monomer, 17 kDa Saccharomyces cerevisiae protein
Buffer: 25 mM HEPES, 500 mM NaCl, 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2013 Apr 25
Structural analysis reveals the flexible C-terminus of Nop15 undergoes rearrangement to recognize a pre-ribosomal RNA folding intermediate. Nucleic Acids Res 45(5):2829-2837 (2017)
Zhang J, Gonzalez LE, Hall TMT
RgGuinier 2.4 nm
Dmax 10.3 nm
VolumePorod 38 nm3

SASDBC7 – Human NEI like DNA glycosylase 1 (NEIL1)

Endonuclease 8-like 1 experimental SAS data
GASBOR model
Sample: Endonuclease 8-like 1 monomer, 45 kDa Homo sapiens protein
Buffer: 25mM HEPES 300mM NaCl 1mM DTT 10% Glycerol, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2015 Mar 13
Destabilization of the PCNA trimer mediated by its interaction with the NEIL1 DNA glycosylase. Nucleic Acids Res 45(5):2897-2909 (2017)
Prakash A, Moharana K, Wallace SS, Doublié S
RgGuinier 3.6 nm
Dmax 15.0 nm
VolumePorod 81 nm3

SASDBD7 – Human Proliferating Cell Nuclear Antigen (PCNA)

Proliferating cell nuclear antigen experimental SAS data
GASBOR model
Sample: Proliferating cell nuclear antigen trimer, 89 kDa Homo sapiens protein
Buffer: 25mM HEPES 100mM NaCl 1mM DTT, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Jan 20
Destabilization of the PCNA trimer mediated by its interaction with the NEIL1 DNA glycosylase. Nucleic Acids Res 45(5):2897-2909 (2017)
Prakash A, Moharana K, Wallace SS, Doublié S
RgGuinier 3.4 nm
Dmax 9.7 nm
VolumePorod 128 nm3

SASDC83 – Dimeric apoptosis regulator BAX (Bcl-2 associated X)

Apoptosis regulator BAX (Bcl-2 associated X) experimental SAS data
CORAL model
Sample: Apoptosis regulator BAX (Bcl-2 associated X) dimer, 42 kDa Homo sapiens protein
Buffer: 20mM sodium phosphate 100mM NaCl, pH: 8
Experiment: SAXS data collected at 23A, Taiwan Photon Source, NSRRC on 2015 May 28
Oligomerization process of Bcl-2 associated X protein revealed from intermediate structures in solution. Phys Chem Chem Phys 19(11):7947-7954 (2017)
Shih O, Yeh YQ, Liao KF, Sung TC, Chiang YW, Jeng US
RgGuinier 3.0 nm
Dmax 9.5 nm
VolumePorod 86 nm3