Browse by MACROMOLECULE type: protein

SASDQG9 – GRP94 protein (Endoplasmin) bound to ADP

Endoplasmin experimental SAS data
Endoplasmin Kratky plot
Sample: Endoplasmin dimer, 169 kDa Mus musculus protein
Buffer: 25 mM HEPES, 200 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2018 Jul 11
Visualization of conformational transition of GRP94 in solution. Life Sci Alliance 7(2) (2024)
Sun S, Zhu R, Zhu M, Wang Q, Li N, Yang B
RgGuinier 5.7 nm
Dmax 24.0 nm
VolumePorod 274 nm3

SASDQH9 – GRP94 protein (Endoplasmin) bound to AMP-PNP

Endoplasmin experimental SAS data
Endoplasmin Kratky plot
Sample: Endoplasmin dimer, 169 kDa Mus musculus protein
Buffer: 25 mM HEPES, 200 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2018 Jul 11
Visualization of conformational transition of GRP94 in solution. Life Sci Alliance 7(2) (2024)
Sun S, Zhu R, Zhu M, Wang Q, Li N, Yang B
RgGuinier 5.5 nm
Dmax 24.5 nm
VolumePorod 260 nm3

SASDUK2 – Histone deacetylase 7

Histone deacetylase 7 experimental SAS data
COOT model
Sample: Histone deacetylase 7 monomer, 44 kDa Arabidopsis thaliana protein
Buffer: 20 mM Tris, 150 mM NaCl, 1 mM β-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2023 Jan 24
Structure-function analyses reveal Arabidopsis thaliana HDA7 to be an inactive histone deacetylase Current Research in Structural Biology :100136 (2024)
Saharan K, Baral S, Shaikh N, Vasudevan D
RgGuinier 3.0 nm
Dmax 10.2 nm
VolumePorod 85 nm3

SASDQ98 – Full-length human DPCD

Protein DPCD experimental SAS data
Full-length human DPCD Rg histogram
Sample: Protein DPCD monomer, 23 kDa Homo sapiens protein
Buffer: 25 mM Tris-HCl, 200 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2018 Nov 8
DPCD is a regulator of R2TP in ciliogenesis initiation through Akt signaling. Cell Rep 43(2):113713 (2024)
Mao YQ, Seraphim TV, Wan Y, Wu R, Coyaud E, Bin Munim M, Mollica A, Laurent E, Babu M, Mennella V, Raught B, Houry WA
RgGuinier 2.1 nm
Dmax 7.8 nm
VolumePorod 28 nm3

SASDRD8 – Drosophila melanogaster kinesin heavy chain

Kinesin heavy chain (A515T) experimental SAS data
Kinesin heavy chain (A515T) Kratky plot
Sample: Kinesin heavy chain (A515T) dimer, 221 kDa Drosophila melanogaster protein
Buffer: 25 mM HEPES/KOH, 150 mM NaCl, 1 mM MgCl2, 2 mM DTT, pH: 7.3
Experiment: SAXS data collected at BM29, ESRF on 2022 Feb 19
Tropomyosin 1-I/C coordinates kinesin-1 and dynein motors during oskar mRNA transport. Nat Struct Mol Biol (2024)
Heber S, McClintock MA, Simon B, Mehtab E, Lapouge K, Hennig J, Bullock SL, Ephrussi A
RgGuinier 10.0 nm
Dmax 45.0 nm
VolumePorod 920 nm3

SASDRE8 – Drosophila melanogaster kinesin heavy chain + Tropomyosin1-I/C

Kinesin heavy chain (A515T)GH09289p experimental SAS data
Kinesin heavy chain (A515T) GH09289p Kratky plot
Sample: Kinesin heavy chain (A515T) dimer, 221 kDa Drosophila melanogaster protein
GH09289p dimer, 96 kDa Drosophila melanogaster protein
Buffer: 25 mM HEPES/KOH, 150 mM NaCl, 1 mM MgCl2, 2 mM DTT, pH: 7.3
Experiment: SAXS data collected at BM29, ESRF on 2022 Feb 19
Tropomyosin 1-I/C coordinates kinesin-1 and dynein motors during oskar mRNA transport. Nat Struct Mol Biol (2024)
Heber S, McClintock MA, Simon B, Mehtab E, Lapouge K, Hennig J, Bullock SL, Ephrussi A
RgGuinier 8.7 nm
Dmax 45.0 nm
VolumePorod 840 nm3

SASDTE2 – human CELSR1 EC4-7

Cadherin EGF LAG seven-pass G-type receptor 1 experimental SAS data
DAMFILT model
Sample: Cadherin EGF LAG seven-pass G-type receptor 1 monomer, 49 kDa Homo sapiens protein
Buffer: 20 mM Tris-HCl, 150 mM KCl, 50 mM NaCl, 2 mM CaCl2, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 Jul 1
CELSR1, a core planar cell polarity protein, features a weakly adhesive and flexible cadherin ectodomain. Structure (2024)
Tamilselvan E, Sotomayor M
RgGuinier 4.9 nm
Dmax 16.9 nm
VolumePorod 85 nm3

SASDT82 – Halophilic Lrp transcription factor

AsnC family transcriptional regulator experimental SAS data
PHENIX model
Sample: AsnC family transcriptional regulator dimer, 36 kDa Haloferax mediterranei (strain … protein
Buffer: 50 mM Tris, 1.5 M NaCl, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2021 Nov 12
Global Lrp regulator protein from Haloferax mediterranei: Transcriptional analysis and structural characterization. Int J Biol Macromol :129541 (2024)
Matarredona L, García-Bonete MJ, Guío J, Camacho M, Fillat MF, Esclapez J, Bonete MJ
RgGuinier 2.4 nm
Dmax 7.9 nm
VolumePorod 70 nm3

SASDEU9 – Legionella pneumophila Phosphocholinase AnkX

Phosphocholinase AnkX experimental SAS data
GASBOR model
Sample: Phosphocholinase AnkX monomer, 107 kDa Legionella pneumophila protein
Buffer: 300 mM NaCl, 2 mM 2-mercaptoethanol and 30 mM Tris-HCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Oct 20
Legionella pneumophila effector AnkX
Wenhua Zhang
RgGuinier 4.3 nm
Dmax 14.7 nm
VolumePorod 145 nm3

SASDR29 – Single-chain Archaeoglobus fulgidus argonaute fAfAgo-fusion variant in high salt buffer

single-chain full Archaeoglobus fulgidus Argonaute variant experimental SAS data
OTHER model
Sample: Single-chain full Archaeoglobus fulgidus Argonaute variant monomer, 78 kDa Archaeoglobus fulgidus protein
Buffer: 20 mM TrisHCl pH 7.5, 500 mM NaCl, 5 mM MgCl2, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Aug 31
The missing part: the Archaeoglobus fulgidus Argonaute forms a functional heterodimer with an N-L1-L2 domain protein. Nucleic Acids Res (2024)
Manakova E, Golovinas E, Pocevičiūtė R, Sasnauskas G, Silanskas A, Rutkauskas D, Jankunec M, Zagorskaitė E, Jurgelaitis E, Grybauskas A, Venclovas Č, Zaremba M
RgGuinier 3.1 nm
Dmax 9.7 nm
VolumePorod 142 nm3