Small Angle Scattering Biological Data Bank SASBDB

Browse by ORGANISM: Homo sapiens (Human)

SASDGD2 – Putative mitochondrial transferase CAF17: human IBA57

Putative transferase CAF17, mitochondrial experimental SAS data

Sample:	Putative transferase CAF17, mitochondrial monomer, 33 kDa Homo sapiens protein
Buffer:	50 mM phosphate, 150 mM NaCl, 5 mM DTT, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Jul 10

Structural properties of [2Fe-2S] ISCA2-IBA57: a complex of the mitochondrial iron-sulfur cluster assembly machinery Scientific Reports 9(1) (2019)
Nasta V, Da Vela S, Gourdoupis S, Ciofi-Baffoni S, Svergun D, Banci L

R_g^Guinier	2.2	nm
D_max	7.1	nm
Volume^Porod	50	nm³

SASDGE2 – Human mitochondrial iron-sulfur cluster assembly ISCA2 protein

Iron-sulfur cluster assembly 2 homolog, mitochondrial experimental SAS data

Sample:	Iron-sulfur cluster assembly 2 homolog, mitochondrial dimer, 24 kDa Homo sapiens protein
Buffer:	50 mM phosphate, 150 mM NaCl, 5 mM DTT, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Sep 16

R_g^Guinier	2.0	nm
D_max	6.7	nm
Volume^Porod	31	nm³

SASDGF2 – Putative mitochondrial transferase CAF17 bound to mitochondrial iron-sulfur cluster assembly ISCA2 protein (human IBA57-ISCA2 complex)

Putative transferase CAF17, mitochondrialIron-sulfur cluster assembly 2 homolog, mitochondrial experimental SAS data

Sample:	Putative transferase CAF17, mitochondrial monomer, 33 kDa Homo sapiens protein Iron-sulfur cluster assembly 2 homolog, mitochondrial dimer, 24 kDa Homo sapiens protein
Buffer:	50 mM phosphate, 150 mM NaCl, 5 mM DTT, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Nov 22

R_g^Guinier	4.0	nm
D_max	16.4	nm
Volume^Porod	114	nm³

SASDF53 – Bruton's Tyrosine Kinase - kinase domain

Bruton's tyrosine kinase, kinase domain experimental SAS data

Sample:	Bruton's tyrosine kinase, kinase domain monomer, 32 kDa Homo sapiens protein
Buffer:	20mM Tris, 150mM NaCl, 1mM TCEP, 5% glycerol, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2018 May 11

Btk SH2-kinase interface is critical for allosteric kinase activation and its targeting inhibits B-cell neoplasms Nature Communications 11(1) (2020)
Duarte D, Lamontanara A, La Sala G, Jeong S, Sohn Y, Panjkovich A, Georgeon S, Kükenshöner T, Marcaida M, Pojer F, De Vivo M, Svergun D, Kim H, Dal Peraro M, Hantschel O

R_g^Guinier	2.1	nm
D_max	6.8	nm
Volume^Porod	52	nm³

SASDF63 – Bruton's Tyrosine Kinase - SH2-kinase domain

Bruton's tyrosine kinase - Src homolgy domain 2 - kinase domain experimental SAS data

Bruton's Tyrosine Kinase - SH2-kinase domain Rg histogram

Sample:	Bruton's tyrosine kinase - Src homolgy domain 2 - kinase domain monomer, 46 kDa Homo sapiens protein
Buffer:	20mM Tris, 150mM NaCl, 1mM TCEP, 5% glycerol, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2018 Nov 3

R_g^Guinier	2.8	nm
D_max	9.4	nm
Volume^Porod	64	nm³

SASDF73 – Bruton's Tyrosine Kinase - SH3-SH2-kinase domain

Bruton's tyrosine kinase - Src homology 3-2 kinase domain experimental SAS data

Bruton's Tyrosine Kinase - SH3-SH2-kinase domain Rg histogram

Sample:	Bruton's tyrosine kinase - Src homology 3-2 kinase domain monomer, 52 kDa Homo sapiens protein
Buffer:	20mM Tris, 150mM NaCl, 1mM TCEP, 5% glycerol, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2018 Nov 2

R_g^Guinier	2.6	nm
D_max	8.3	nm
Volume^Porod	72	nm³

SASDF83 – Bruton's Tyrosine Kinase - Full-length

Bruton's tyrosine kinase - full length experimental SAS data

Bruton's Tyrosine Kinase - Full-length Rg histogram

Sample:	Bruton's tyrosine kinase - full length monomer, 77 kDa Homo sapiens protein
Buffer:	20mM Tris, 150mM NaCl, 1mM TCEP, 5% glycerol, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2018 Nov 3

R_g^Guinier	4.0	nm
D_max	15.6	nm
Volume^Porod	114	nm³

SASDFK8 – GON7, the fifth subunit of human KEOPS

EKC/KEOPS complex subunit GON7 experimental SAS data

EKC/KEOPS complex subunit GON7 Kratky plot

Sample:	EKC/KEOPS complex subunit GON7 monomer, 13 kDa Homo sapiens protein
Buffer:	20 mM MES, 200 mM NaCl, 5 mM β-mercaptoethanol, pH: 6.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2017 Mar 26

Defects in t⁶A tRNA modification due to GON7 and YRDC mutations lead to Galloway-Mowat syndrome. Nat Commun 10(1):3967 (2019)
Arrondel C, Missoury S, Snoek R, Patat J, Menara G, Collinet B, Liger D, Durand D, Gribouval O, Boyer O, Buscara L, Martin G, Machuca E, Nevo F, Lescop E, Braun DA, Boschat AC, Sanquer S, Guerrera IC, Revy P, Parisot M, Masson C, Boddaert N, Charbit M, Decramer S, Novo R, Macher MA, Ranchin B, Bacchetta J, Laurent A, Collardeau-Frachon S, van Eerde AM, Hildebrandt F, Magen D, Antignac C, van Tilbeurgh H, Mollet G

R_g^Guinier	3.1	nm
D_max	12.5	nm
Volume^Porod	46	nm³

SASDFL8 – GON7, the fifth subunit of human KEOPS, bound to LAGE3-OSGEP (EKC/KEOPS complex subunit:Probable tRNA N6-adenosine threonylcarbamoyltransferase)

EKC/KEOPS complex subunit GON7EKC/KEOPS complex subunit LAGE3Probable tRNA N6-adenosine threonylcarbamoyltransferase experimental SAS data

Sample:	EKC/KEOPS complex subunit GON7 monomer, 12 kDa Homo sapiens protein EKC/KEOPS complex subunit LAGE3 monomer, 15 kDa Homo sapiens protein Probable tRNA N6-adenosine threonylcarbamoyltransferase monomer, 36 kDa Homo sapiens protein
Buffer:	20 mM MES, 200 mM NaCl, 5 mM β-mercaptoethanol, pH: 6.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2017 May 12

R_g^Guinier	3.1	nm
D_max	11.5	nm
Volume^Porod	91	nm³

SASDFM8 – GON7, the fifth subunit of human KEOPS, bound to the EKC/KEOPS complex subunit, LAGE3

EKC/KEOPS complex subunit GON7EKC/KEOPS complex subunit LAGE3 experimental SAS data

EKC/KEOPS complex subunit GON7 EKC/KEOPS complex subunit LAGE3 Kratky plot

Sample:	EKC/KEOPS complex subunit GON7 dimer, 25 kDa Homo sapiens protein EKC/KEOPS complex subunit LAGE3 dimer, 33 kDa Homo sapiens protein
Buffer:	20 mM MES, 200 mM NaCl, 5 mM β-mercaptoethanol, pH: 6.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2017 Mar 19

R_g^Guinier	3.6	nm
D_max	15.5	nm
Volume^Porod	126	nm³

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