Small Angle Scattering Biological Data Bank SASBDB

Browse by ORGANISM: Homo sapiens (Human)

SASDNL6 – Retinoblastoma protein at 2 mg/ml

Retinoblastoma-associated protein experimental SAS data

Retinoblastoma-associated protein Kratky plot

Sample:	Retinoblastoma-associated protein monomer, 41 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate pH 7.0, 200 mM NaCl, 1mM DTT, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Jul 14

Conformational buffering underlies functional selection in intrinsically disordered protein regions. Nat Struct Mol Biol (2022)
González-Foutel NS, Glavina J, Borcherds WM, Safranchik M, Barrera-Vilarmau S, Sagar A, Estaña A, Barozet A, Garrone NA, Fernandez-Ballester G, Blanes-Mira C, Sánchez IE, de Prat-Gay G, Cortés J, Bernadó P, Pappu RV, Holehouse AS, Daughdrill GW, Chemes LB

R_g^Guinier	2.5	nm
D_max	7.9	nm
Volume^Porod	64	nm³

SASDNM6 – Retinoblastoma protein at 4 mg/ml

Sample:	Retinoblastoma-associated protein monomer, 41 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate pH 7.0, 200 mM NaCl, 1mM DTT, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Jul 14

R_g^Guinier	2.6	nm
D_max	10.0	nm
Volume^Porod	68	nm³

SASDNR6 – Retinoblastoma protein/Early E1A protein (RB-E1A) complex at 0.7 mg/ml

Retinoblastoma-associated proteinEarly E1A protein experimental SAS data

Retinoblastoma-associated protein Early E1A protein Kratky plot

Sample:	Retinoblastoma-associated protein monomer, 41 kDa Homo sapiens protein Early E1A protein monomer, 13 kDa Human adenovirus C … protein
Buffer:	20 mM sodium phosphate pH 7.0, 200 mM NaCl, 1mM DTT, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Jul 14

R_g^Guinier	2.9	nm
D_max	10.0	nm
Volume^Porod	80	nm³

SASDNS6 – Retinoblastoma protein/Early E1A protein (RB-E1A) complex at 1.4 mg/ml

Sample:	Retinoblastoma-associated protein monomer, 41 kDa Homo sapiens protein Early E1A protein monomer, 13 kDa Human adenovirus C … protein
Buffer:	20 mM sodium phosphate pH 7.0, 200 mM NaCl, 1mM DTT, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Jul 14

R_g^Guinier	3.0	nm
D_max	12.0	nm
Volume^Porod	84	nm³

SASDNT6 – Retinoblastoma protein/Early E1A protein (RB-E1A) complex at 2.7 mg/ml

Sample:	Retinoblastoma-associated protein monomer, 41 kDa Homo sapiens protein Early E1A protein monomer, 13 kDa Human adenovirus C … protein
Buffer:	20 mM sodium phosphate pH 7.0, 200 mM NaCl, 1mM DTT, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Jul 14

R_g^Guinier	3.3	nm
D_max	17.0	nm
Volume^Porod	93	nm³

SASDNU6 – Retinoblastoma protein/Early E1A protein (RB-E1A) complex - merged data from concentration series measurements

Sample:	Retinoblastoma-associated protein monomer, 41 kDa Homo sapiens protein Early E1A protein monomer, 13 kDa Human adenovirus C … protein
Buffer:	20 mM sodium phosphate pH 7.0, 200 mM NaCl, 1mM DTT, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Jul 14

R_g^Guinier	3.0	nm
D_max	12.0	nm
Volume^Porod	84	nm³

SASDNX3 – Calcium-bound Calmodulin, including structural models

Calcium-bound Calmodulin, including structural models Rg histogram

Sample:	Calmodulin-1 monomer, 17 kDa Homo sapiens protein
Buffer:	20 mM Hepes, 150 mM NaCl, 2 mM CaCl2, pH: 7.4
Experiment:	SAXS data collected at SWING, SOLEIL on 2020 Nov 15

Dynamics and structural changes of calmodulin upon interaction with the antagonist calmidazolium. BMC Biol 20(1):176 (2022)
Léger C, Pitard I, Sadi M, Carvalho N, Brier S, Mechaly A, Raoux-Barbot D, Davi M, Hoos S, Weber P, Vachette P, Durand D, Haouz A, Guijarro JI, Ladant D, Chenal A

R_g^Guinier	2.2	nm
D_max	7.2	nm
Volume^Porod	33	nm³

SASDNY3 – Calcium-bound Calmodulin complexed with Calmidazolium

Calmodulin-1Calmidazolium experimental SAS data

Sample:	Calmodulin-1 monomer, 17 kDa Homo sapiens protein Calmidazolium monomer, 1 kDa
Buffer:	20 mM Hepes, 150 mM NaCl, 2 mM CaCl2, pH: 7.4
Experiment:	SAXS data collected at SWING, SOLEIL on 2020 Nov 15

R_g^Guinier	1.7	nm
D_max	5.2	nm
Volume^Porod	30	nm³

SASDN97 – Human thryoglobulin measured using SEC-SAXS

Sample:	Thyroglobulin dimer, 610 kDa Homo sapiens protein
Buffer:	phosphate buffered saline, 150mM NaCl, pH: 8
Experiment:	SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2020 Jul 17

Thyroglobulin - human and bovine SAXS/WAXS data
James Byrnes

R_g^Guinier	7.7	nm
D_max	26.3	nm
Volume^Porod	1800	nm³

SASDJJ5 – Histone H2A:H2B:H3:H4 complex with aprataxin and polynucleotide kinase like factor (APLF) acidic domain

Aprataxin and PNK-like factor (acidic domain)Histone H2AHistone H2BHistone H3Histone H4 experimental SAS data

Sample:	Aprataxin and PNK-like factor (acidic domain) dimer, 15 kDa Homo sapiens protein Histone H2A dimer, 26 kDa Drosophila melanogaster protein Histone H2B dimer, 27 kDa Drosophila melanogaster protein Histone H3 dimer, 30 kDa Drosophila melanogaster protein Histone H4 dimer, 23 kDa Drosophila melanogaster protein
Buffer:	25 mM NaPi, 300 mM NaCl, 3% v/v glycerol, 1 mM DTT,, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Jul 10

Chaperoning of the histone octamer by the acidic domain of DNA repair factor APLF. Sci Adv 8(30):eabo0517 (2022)
Corbeski I, Guo X, Eckhardt BV, Fasci D, Wiegant W, Graewert MA, Vreeken K, Wienk H, Svergun DI, Heck AJR, van Attikum H, Boelens R, Sixma TK, Mattiroli F, van Ingen H

R_g^Guinier	3.7	nm
D_max	10.8	nm
Volume^Porod	260	nm³

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