Small Angle Scattering Biological Data Bank SASBDB

Browse by ORGANISM: Homo sapiens (Human)

SASDJ93 – Ubiquitin carboxyl-terminal hydrolase, MINDY2, wild-type apo-form

Ubiquitin carboxyl-terminal hydrolase MINDY-2 experimental SAS data

Sample:	Ubiquitin carboxyl-terminal hydrolase MINDY-2 monomer, 31 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 5 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Nov 23

Mechanism of activation and regulation of deubiquitinase activity in MINDY1 and MINDY2. Mol Cell (2021)
Abdul Rehman SA, Armstrong LA, Lange SM, Kristariyanto YA, Gräwert TW, Knebel A, Svergun DI, Kulathu Y

R_g^Guinier	2.1	nm
D_max	6.5	nm
Volume^Porod	45	nm³

SASDJA3 – Ubiquitin carboxyl-terminal hydrolase, MINDY2, wild-type bound to mono-ubiquitin

Ubiquitin carboxyl-terminal hydrolase MINDY-2Polyubiquitin-C experimental SAS data

Sample:	Ubiquitin carboxyl-terminal hydrolase MINDY-2 monomer, 31 kDa Homo sapiens protein Polyubiquitin-C monomer, 9 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 5 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Nov 23

R_g^Guinier	2.3	nm
D_max	6.8	nm
Volume^Porod	58	nm³

SASDJB3 – Ubiquitin carboxyl-terminal hydrolase, MINDY2 (C266A mutant), bound to di-ubiquitin

Ubiquitin carboxyl-terminal hydrolase C266A mutantPolyubiquitin-C experimental SAS data

Sample:	Ubiquitin carboxyl-terminal hydrolase C266A mutant monomer, 31 kDa Homo sapiens protein Polyubiquitin-C dimer, 17 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 5 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Nov 23

R_g^Guinier	2.5	nm
D_max	8.0	nm
Volume^Porod	68	nm³

SASDJC3 – Ubiquitin carboxyl-terminal hydrolase, MINDY2 (C266A mutant), bound to tri-ubiquitin

Sample:	Ubiquitin carboxyl-terminal hydrolase C266A mutant monomer, 31 kDa Homo sapiens protein Polyubiquitin-C trimer, 26 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 5 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 May 26

R_g^Guinier	2.8	nm
D_max	8.8	nm
Volume^Porod	97	nm³

SASDJD3 – Ubiquitin carboxyl-terminal hydrolase, MINDY2 (C266A mutant), bound to tetra-ubiquitin

Sample:	Ubiquitin carboxyl-terminal hydrolase C266A mutant monomer, 31 kDa Homo sapiens protein Polyubiquitin-C tetramer, 34 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 5 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 May 26

R_g^Guinier	2.9	nm
D_max	9.2	nm
Volume^Porod	111	nm³

SASDJE3 – Ubiquitin carboxyl-terminal hydrolase, MINDY2 (C266A mutant), bound to penta-ubiquitin

Sample:	Ubiquitin carboxyl-terminal hydrolase C266A mutant monomer, 31 kDa Homo sapiens protein Polyubiquitin-C pentamer, 43 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 5 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 May 26

R_g^Guinier	2.8	nm
D_max	9.0	nm
Volume^Porod	116	nm³

SASDGG6 – Ubiquitin activating enzyme 5 (0.3 mg/ml)

Ubiquitin-like modifier-activating enzyme 5 experimental SAS data

Sample:	Ubiquitin-like modifier-activating enzyme 5 dimer, 68 kDa Homo sapiens protein
Buffer:	20 mM Tris, 150 mM NaCl, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Jun 13

Structure and dynamics of UBA5-UFM1 complex formation showing new insights in the UBA5 activation mechanism Journal of Structural Biology :107796 (2021)
Fuchs S, Kikhney A, Schubert R, Kaiser C, Liebau E, Svergun D, Betzel C, Perbandt M

R_g^Guinier	2.8	nm
D_max	9.0	nm
Volume^Porod	74	nm³

SASDGH6 – Ubiquitin activating enzyme 5 (1.0 mg/ml)

Sample:	Ubiquitin-like modifier-activating enzyme 5 dimer, 68 kDa Homo sapiens protein
Buffer:	20 mM Tris, 150 mM NaCl, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Jun 13

R_g^Guinier	2.9	nm
D_max	10.0	nm
Volume^Porod	82	nm³

SASDGJ6 – Ubiquitin activating enzyme 5 (6.8 mg/ml)

Sample:	Ubiquitin-like modifier-activating enzyme 5 dimer, 68 kDa Homo sapiens protein
Buffer:	20 mM Tris, 150 mM NaCl, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Jun 13

R_g^Guinier	3.2	nm
D_max	12.0	nm
Volume^Porod	108	nm³

SASDGK6 – Ubiquitin activating enzyme 5 (4.4 mg/ml)

Sample:	Ubiquitin-like modifier-activating enzyme 5 dimer, 68 kDa Homo sapiens protein
Buffer:	20 mM Tris, 150 mM NaCl, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Jun 13

R_g^Guinier	3.1	nm
D_max	12.0	nm
Volume^Porod	104	nm³

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