Browse by ORGANISM: Homo sapiens (Human)

SASDKW9 – Transcription factors USF1 and NF-Y bound to a CCAAT DNA box(50bp)

Upstream stimulatory factor 1Nuclear transcription factor Y subunit alphaNuclear transcription factor Y subunit betaNuclear transcription factor Y subunit gammaDNA 50bp experimental SAS data
DAMMIN model
Sample: Upstream stimulatory factor 1 dimer, 50 kDa Homo sapiens protein
Nuclear transcription factor Y subunit alpha monomer, 10 kDa Homo sapiens protein
Nuclear transcription factor Y subunit beta monomer, 11 kDa Homo sapiens protein
Nuclear transcription factor Y subunit gamma monomer, 11 kDa Homo sapiens protein
DNA 50bp monomer, 31 kDa DNA
Buffer: 100 mM cacodylate buffer, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jun 14
The USR domain of USF1 mediates NF-Y interactions and cooperative DNA binding International Journal of Biological Macromolecules (2021)
Bernardini A, Lorenzo M, Chaves-Sanjuan A, Swuec P, Pigni M, Saad D, Konarev P, Graewert M, Valentini E, Svergun D, Nardini M, Mantovani R, Gnesutta N
RgGuinier 4.8 nm
Dmax 17.0 nm
VolumePorod 165 nm3

SASDKX9 – Transcription factors MAX and NF-Y bound to a CCAAT DNA box(48bp)

Protein max (Isoform 2, short, 13-21: missing)Nuclear transcription factor Y subunit alphaNuclear transcription factor Y subunit betaNuclear transcription factor Y subunit gammaDNA 48bp experimental SAS data
DAMMIN model
Sample: Protein max (Isoform 2, short, 13-21: missing) dimer, 39 kDa Homo sapiens protein
Nuclear transcription factor Y subunit alpha monomer, 10 kDa Homo sapiens protein
Nuclear transcription factor Y subunit beta monomer, 11 kDa Homo sapiens protein
Nuclear transcription factor Y subunit gamma monomer, 11 kDa Homo sapiens protein
DNA 48bp monomer, 30 kDa DNA
Buffer: 100 mM cacodylate buffer, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2012 Dec 10
The USR domain of USF1 mediates NF-Y interactions and cooperative DNA binding International Journal of Biological Macromolecules (2021)
Bernardini A, Lorenzo M, Chaves-Sanjuan A, Swuec P, Pigni M, Saad D, Konarev P, Graewert M, Valentini E, Svergun D, Nardini M, Mantovani R, Gnesutta N
RgGuinier 5.2 nm
Dmax 17.0 nm
VolumePorod 151 nm3

SASDFM4 – Matrix metalloproteinase-1 (MMP-1)

Matrix metalloproteinase-1 (Interstitial collagenase) experimental SAS data
Matrix metalloproteinase-1 (Interstitial collagenase) Kratky plot
Sample: Matrix metalloproteinase-1 (Interstitial collagenase) monomer, 43 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl, 150 mM Sodium chloride, 10 mM Calcium chloride, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2016 May 14
Conformationally constrained mutant of human matrix metalloproteinase-1
Rob Holland
RgGuinier 2.6 nm
Dmax 8.3 nm
VolumePorod 50 nm3

SASDFN4 – Domain-stapled mutant of Matrix metalloproteinase-1 (MMP-1 S243C, S318C)

Matrix metalloproteinase-1 (Interstitial collagenase) (S243C, S318C) experimental SAS data
Matrix metalloproteinase-1 (Interstitial collagenase) (S243C, S318C) Kratky plot
Sample: Matrix metalloproteinase-1 (Interstitial collagenase) (S243C, S318C) monomer, 43 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl, 150 mM Sodium chloride, 10 mM Calcium chloride, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2016 May 14
Conformationally constrained mutant of human matrix metalloproteinase-1
Rob Holland
RgGuinier 2.6 nm
Dmax 7.7 nm
VolumePorod 43 nm3

SASDFP4 – Pro-matrix metalloproteinase-1 (MMP-1)

Pro-matrix metalloproteinase-1 (Interstitial collagenase) experimental SAS data
Pro-matrix metalloproteinase-1 (Interstitial collagenase) Kratky plot
Sample: Pro-matrix metalloproteinase-1 (Interstitial collagenase) monomer, 52 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl, 150 mM Sodium chloride, 10 mM Calcium chloride, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2015 May 21
Conformationally constrained mutant of human matrix metalloproteinase-1
Rob Holland
RgGuinier 2.7 nm
Dmax 9.0 nm
VolumePorod 73 nm3

SASDFQ4 – Domain-stapled mutant of Pro-Matrix metalloproteinase-1 (proMMP-1 S243C, S318C)

Pro-matrix metalloproteinase-1 (Interstitial collagenase) (proMMP-1 S243C, S318C) experimental SAS data
Pro-matrix metalloproteinase-1 (Interstitial collagenase) (proMMP-1 S243C, S318C) Kratky plot
Sample: Pro-matrix metalloproteinase-1 (Interstitial collagenase) (proMMP-1 S243C, S318C) monomer, 52 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl, 150 mM Sodium chloride, 10 mM Calcium chloride, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2015 May 21
Conformationally constrained mutant of human matrix metalloproteinase-1
Rob Holland
RgGuinier 2.7 nm
Dmax 8.9 nm
VolumePorod 61 nm3

SASDGZ5 – Suppressor of Fused from Human D30

Suppressor of fused homolog experimental SAS data
CUSTOM IN-HOUSE model
Sample: Suppressor of fused homolog monomer, 51 kDa Homo sapiens protein
Buffer: 50 mM bis-TRIS pH 5.5, 200 mM NaCl, 10% glycerol, pH: 5.5
Experiment: SAXS data collected at SWING, SOLEIL on 2018 Jun 30
Suppressor of Fused
Valerie Biou
RgGuinier 3.2 nm
Dmax 14.0 nm

SASDEL7 – Human glycine decarboxylase

Glycine decarboxylase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Glycine decarboxylase dimer, 219 kDa Homo sapiens protein
Buffer: 10 mM TRIS pH 7.5, 100 mM NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Nov 12
Glycine decarboxylase (P-protein of glycine cleavage system)
Bart Van Laer
RgGuinier 3.9 nm
VolumePorod 304 nm3

SASDEM7 – Human glycine decarboxylase with glycine

Glycine decarboxylase experimental SAS data
Glycine decarboxylase Kratky plot
Sample: Glycine decarboxylase dimer, 219 kDa Homo sapiens protein
Buffer: 10 mM TRIS pH 7.5, 100 mM NaCl, 1 mM DTT , 100 mM glycine, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Nov 12
Glycine decarboxylase (P-protein of glycine cleavage system)
Bart Van Laer
RgGuinier 4.0 nm
VolumePorod 315 nm3

SASDEN7 – Neanderthal glycine decarboxylase

Glycine decarboxylase experimental SAS data
Glycine decarboxylase Kratky plot
Sample: Glycine decarboxylase dimer, 219 kDa Homo sapiens neanderthalensis protein
Buffer: 10 mM TRIS pH 7.5, 100 mM NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Nov 12
Glycine decarboxylase (P-protein of glycine cleavage system)
Bart Van Laer
RgGuinier 4.0 nm
VolumePorod 309 nm3