Browse by ORGANISM: Homo sapiens (Human)

SASDJK6 – Sarcomeric intrinsically disordered protein FATZ-1 (Δ91-FATZ-1)

Δ91 construct of FATZ-1 (alias myozenin-1 or calsarcin-2) experimental SAS data
Sarcomeric intrinsically disordered protein FATZ-1 (Δ91-FATZ-1) Rg histogram
Sample: Δ91 construct of FATZ-1 (alias myozenin-1 or calsarcin-2) monomer, 22 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Jul 18
Order from disorder in the sarcomere: FATZ forms a fuzzy but tight complex and phase-separated condensates with α-actinin. Sci Adv 7(22) (2021)
Sponga A, Arolas JL, Schwarz TC, Jeffries CM, Rodriguez Chamorro A, Kostan J, Ghisleni A, Drepper F, Polyansky A, De Almeida Ribeiro E, Pedron M, Zawadzka-Kazimierczuk A, Mlynek G, Peterbauer T, Doto P, Schreiner C, Hollerl E, Mateos B, Geist L, Faulkner G, Kozminski W, Svergun DI, Warscheid B, Zagrovic B, Gautel M, Konrat R, Djinović-Carugo K
RgGuinier 3.9 nm
Dmax 17.3 nm
VolumePorod 66 nm3

SASDJL6 – Sarcomeric F-actin crosslinking protein α-actinin-2 (spectrin repeat rod domain, rod-α-actinin-2)

Rod domain of α-actinin-2 experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Rod domain of α-actinin-2 dimer, 112 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Dec 5
Order from disorder in the sarcomere: FATZ forms a fuzzy but tight complex and phase-separated condensates with α-actinin. Sci Adv 7(22) (2021)
Sponga A, Arolas JL, Schwarz TC, Jeffries CM, Rodriguez Chamorro A, Kostan J, Ghisleni A, Drepper F, Polyansky A, De Almeida Ribeiro E, Pedron M, Zawadzka-Kazimierczuk A, Mlynek G, Peterbauer T, Doto P, Schreiner C, Hollerl E, Mateos B, Geist L, Faulkner G, Kozminski W, Svergun DI, Warscheid B, Zagrovic B, Gautel M, Konrat R, Djinović-Carugo K
RgGuinier 6.7 nm
Dmax 27.2 nm
VolumePorod 214 nm3

SASDJM6 – Sarcomeric F-actin crosslinking protein α-actinin-2 (half-dimer, hd)

Half dimer of α-actinin-2 experimental SAS data
Sarcomeric F-actin crosslinking protein α-actinin-2 (half-dimer, hd) Rg histogram
Sample: Half dimer of α-actinin-2 monomer, 107 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 May 18
Order from disorder in the sarcomere: FATZ forms a fuzzy but tight complex and phase-separated condensates with α-actinin. Sci Adv 7(22) (2021)
Sponga A, Arolas JL, Schwarz TC, Jeffries CM, Rodriguez Chamorro A, Kostan J, Ghisleni A, Drepper F, Polyansky A, De Almeida Ribeiro E, Pedron M, Zawadzka-Kazimierczuk A, Mlynek G, Peterbauer T, Doto P, Schreiner C, Hollerl E, Mateos B, Geist L, Faulkner G, Kozminski W, Svergun DI, Warscheid B, Zagrovic B, Gautel M, Konrat R, Djinović-Carugo K
RgGuinier 5.3 nm
Dmax 22.0 nm
VolumePorod 172 nm3

SASDJN6 – Sarcomeric fuzzy α-actinin-2/FATZ-1 complex (rod-α-actinin-2/Δ91-FATZ-1)

Rod domain of α-actinin-2Δ91 construct of FATZ-1 (alias myozenin-1 or calsarcin-2) experimental SAS data
Sarcomeric fuzzy α-actinin-2/FATZ-1 complex (rod-α-actinin-2/Δ91-FATZ-1) Rg histogram
Sample: Rod domain of α-actinin-2 dimer, 112 kDa Homo sapiens protein
Δ91 construct of FATZ-1 (alias myozenin-1 or calsarcin-2) dimer, 43 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 May 18
Order from disorder in the sarcomere: FATZ forms a fuzzy but tight complex and phase-separated condensates with α-actinin. Sci Adv 7(22) (2021)
Sponga A, Arolas JL, Schwarz TC, Jeffries CM, Rodriguez Chamorro A, Kostan J, Ghisleni A, Drepper F, Polyansky A, De Almeida Ribeiro E, Pedron M, Zawadzka-Kazimierczuk A, Mlynek G, Peterbauer T, Doto P, Schreiner C, Hollerl E, Mateos B, Geist L, Faulkner G, Kozminski W, Svergun DI, Warscheid B, Zagrovic B, Gautel M, Konrat R, Djinović-Carugo K
RgGuinier 7.6 nm
Dmax 28.4 nm
VolumePorod 371 nm3

SASDJP6 – Sarcomeric fuzzy α-actinin-2/FATZ-1 complex (hd-α-actinin-2/Δ91-FATZ-1)

Δ91 construct of FATZ-1 (alias myozenin-1 or calsarcin-2)Half dimer of α-actinin-2 experimental SAS data
Sarcomeric fuzzy α-actinin-2/FATZ-1 complex (hd-α-actinin-2/Δ91-FATZ-1) Rg histogram
Sample: Δ91 construct of FATZ-1 (alias myozenin-1 or calsarcin-2) monomer, 22 kDa Homo sapiens protein
Half dimer of α-actinin-2 monomer, 107 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 May 18
Order from disorder in the sarcomere: FATZ forms a fuzzy but tight complex and phase-separated condensates with α-actinin. Sci Adv 7(22) (2021)
Sponga A, Arolas JL, Schwarz TC, Jeffries CM, Rodriguez Chamorro A, Kostan J, Ghisleni A, Drepper F, Polyansky A, De Almeida Ribeiro E, Pedron M, Zawadzka-Kazimierczuk A, Mlynek G, Peterbauer T, Doto P, Schreiner C, Hollerl E, Mateos B, Geist L, Faulkner G, Kozminski W, Svergun DI, Warscheid B, Zagrovic B, Gautel M, Konrat R, Djinović-Carugo K
RgGuinier 5.8 nm
Dmax 22.5 nm
VolumePorod 242 nm3

SASDJ85 – Zinc transporter 1 – hZnT1-CTD

Zinc transporter 1 experimental SAS data
DAMFILT model
Sample: Zinc transporter 1 dimer, 37 kDa Homo sapiens protein
Buffer: 20mM Tris, 150mM NaCl, 1mM TCEP, pH: 7.6
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Aug 14
Heterologous Expression and Biochemical Characterization of the Human Zinc Transporter 1 (ZnT1) and Its Soluble C-Terminal Domain Frontiers in Chemistry 9 (2021)
Cotrim C, Jarrott R, Whitten A, Choudhury H, Drew D, Martin J
RgGuinier 2.7 nm
Dmax 10.0 nm
VolumePorod 50 nm3

SASDK76 – Polymerase delta-interacting protein 2 (POLDIP2, amino acids 51-368)

Polymerase delta-interacting protein 2 experimental SAS data
Polymerase delta-interacting protein 2 Kratky plot
Sample: Polymerase delta-interacting protein 2 monomer, 37 kDa Homo sapiens protein
Buffer: 20 mM HEPES pH 7.5, 500 mM NaCl, 5% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at Bruker Nanostar, University of Huddersfield on 2019 Jul 18
Crystal structure and molecular dynamics of human POLDIP2, a multifaceted adaptor protein in metabolism and genome stability. Protein Sci (2021)
Kulik AA, Maruszczak KK, Thomas DC, Nabi-Aldridge NLA, Carr M, Bingham RJ, Cooper CDO
RgGuinier 2.2 nm
Dmax 7.2 nm
VolumePorod 56 nm3

SASDJL9 – Saposin-A monomer

Prosaposin (Saposin A) experimental SAS data
Prosaposin (Saposin A) Kratky plot
Sample: Prosaposin (Saposin A) monomer, 10 kDa Homo sapiens protein
Buffer: 25 mM Tris-HCl, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Feb 18
Stable Picodisc Assemblies from Saposin Proteins and Branched Detergents. Biochemistry 60(14):1108-1119 (2021)
Kurgan KW, Chen B, Brown KA, Falco Cobra P, Ye X, Ge Y, Gellman SH
RgGuinier 1.5 nm
Dmax 5.0 nm
VolumePorod 23 nm3

SASDJM9 – Saposin-A plus MNA C12 (1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-dodecyl-d-mannitol)

Prosaposin (Saposin-A)1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-dodecyl-d-mannitol experimental SAS data
Prosaposin (Saposin-A) 1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-dodecyl-d-mannitol Kratky plot
Sample: Prosaposin (Saposin-A) dimer, 19 kDa Homo sapiens protein
1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-dodecyl-d-mannitol decamer, 12 kDa
Buffer: 25 mM Tris-HCl, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Feb 18
Stable Picodisc Assemblies from Saposin Proteins and Branched Detergents. Biochemistry 60(14):1108-1119 (2021)
Kurgan KW, Chen B, Brown KA, Falco Cobra P, Ye X, Ge Y, Gellman SH
RgGuinier 2.3 nm
Dmax 6.2 nm

SASDJN9 – Saposin-A plus MNA C13 (1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-tridecyl-d-mannitol)

Prosaposin (Saposin-A)1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-tridecyl-d-mannitol experimental SAS data
Prosaposin (Saposin-A) 1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-tridecyl-d-mannitol Kratky plot
Sample: Prosaposin (Saposin-A) dimer, 19 kDa Homo sapiens protein
1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-tridecyl-d-mannitol decamer, 12 kDa
Buffer: 25 mM Tris-HCl, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Feb 18
Stable Picodisc Assemblies from Saposin Proteins and Branched Detergents. Biochemistry 60(14):1108-1119 (2021)
Kurgan KW, Chen B, Brown KA, Falco Cobra P, Ye X, Ge Y, Gellman SH
RgGuinier 2.5 nm
Dmax 6.7 nm