Browse by ORGANISM: other species

SASDL63 – Gloeobacter violaceus Ligand-Gated Ion Channel (GLIC) at pH 7.5 measured with cuvette-mode SANS

Proton-gated ion channel experimental SAS data
Proton-gated ion channel Kratky plot
Sample: Proton-gated ion channel pentamer, 183 kDa Gloeobacter violaceus (strain … protein
Buffer: D2O, 20 mM Tris, 150 mM NaCl, 0.5 mM matched-out deuterated DDM,, pH: 7.5
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2019 Jun 21
Probing solution structure of the pentameric ligand-gated ion channel GLIC by small-angle neutron scattering Proceedings of the National Academy of Sciences 118(37):e2108006118 (2021)
Lycksell M, Rovšnik U, Bergh C, Johansen N, Martel A, Porcar L, Arleth L, Howard R, Lindahl E
RgGuinier 4.0 nm
Dmax 17.7 nm
VolumePorod 225 nm3

SASDL43 – Gloeobacter violaceus Ligand-Gated Ion Channel (GLIC) at pH 3.0 measured with paused-flow SEC-SANS

Proton-gated ion channel experimental SAS data
GROMACS model
Sample: Proton-gated ion channel pentamer, 183 kDa Gloeobacter violaceus (strain … protein
Buffer: D2O, 20 mM citrate, 150 mM NaCl, 0.5 mM match-out deuterated DDM, pH: 3
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2020 Aug 22
Probing solution structure of the pentameric ligand-gated ion channel GLIC by small-angle neutron scattering Proceedings of the National Academy of Sciences 118(37):e2108006118 (2021)
Lycksell M, Rovšnik U, Bergh C, Johansen N, Martel A, Porcar L, Arleth L, Howard R, Lindahl E
RgGuinier 3.8 nm
Dmax 12.7 nm
VolumePorod 279 nm3

SASDLF9 – 200-310 region of Hendra virus P/V/W protein (PNT3)

Protein W experimental SAS data
200-310 region of Hendra virus P/V/W protein (PNT3) Rg histogram
Sample: Protein W monomer, 15 kDa Hendra virus (isolate … protein
Buffer: 50 mM sodium phosphate, 5 mM EDTA, pH: 6.5
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Jun 12
Identification of a Region in the Common Amino-terminal Domain of Hendra Virus P, V, and W Proteins Responsible for Phase Transition and Amyloid Formation Biomolecules 11(9):1324 (2021)
Salladini E, Gondelaud F, Nilsson J, Pesce G, Bignon C, Murrali M, Fabre R, Pierattelli R, Kajava A, Horvat B, Gerlier D, Mathieu C, Longhi S
RgGuinier 3.4 nm
Dmax 15.5 nm
VolumePorod 38 nm3

SASDKR7 – Hunchback mRNA translation repression complex of Brat-NHL, Pum-HD, Nanos-ZnF and hb NRE2 RNA

Brain tumor proteinMaternal protein pumilioProtein nanoshunchback mRNA Nanos Response Element 2 experimental SAS data
Brain tumor protein Maternal protein pumilio Protein nanos hunchback mRNA Nanos Response Element 2 Kratky plot
Sample: Brain tumor protein monomer, 32 kDa Drosophila melanogaster protein
Maternal protein pumilio monomer, 38 kDa Drosophila melanogaster protein
Protein nanos monomer, 11 kDa Drosophila melanogaster protein
Hunchback mRNA Nanos Response Element 2 monomer, 7 kDa Drosophila melanogaster RNA
Buffer: 50 mM Tris, 150 mM NaCl, 1 mM DTT, 3% glycerol, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Nov 12
Structure and dynamics of the quaternary hunchback mRNA translation repression complex. Nucleic Acids Res 49(15):8866-8885 (2021)
Macošek J, Simon B, Linse JB, Jagtap PKA, Winter SL, Foot J, Lapouge K, Perez K, Rettel M, Ivanović MT, Masiewicz P, Murciano B, Savitski MM, Loedige I, Hub JS, Gabel F, Hennig J
RgGuinier 3.7 nm
Dmax 12.7 nm
VolumePorod 114 nm3

SASDK28 – Lipid A phosphoethanolamine transferase in n-dodecyl-phosphocholine micelles

YhbX/YhjW/YijP/YjdB family protein (L152F) experimental SAS data
Lipid A phosphoethanolamine transferase in n-dodecyl-phosphocholine micelles Rg histogram
Sample: YhbX/YhjW/YijP/YjdB family protein (L152F) monomer, 62 kDa Neisseria meningitidis serogroup … protein
Buffer: 50 mM HEPES, 100 mM NaCl, 0.14% Fos-Choline 12 (FC-12), pH: 7
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2015 Nov 9
Conformational flexibility of EptA driven by an interdomain helix provides insights for enzyme-substrate recognition. IUCrJ 8(Pt 5):732-746 (2021)
Anandan A, Dunstan NW, Ryan TM, Mertens HDT, Lim KYL, Evans GL, Kahler CM, Vrielink A
RgGuinier 4.6 nm
Dmax 17.1 nm
VolumePorod 186 nm3

SASDK38 – Lipid A phosphoethanolamine transferase in n-dodecyl-β-D maltoside micelles

YhbX/YhjW/YijP/YjdB family protein (L152F) experimental SAS data
Lipid A phosphoethanolamine transferase in n-dodecyl-β-D maltoside micelles Rg histogram
Sample: YhbX/YhjW/YijP/YjdB family protein (L152F) monomer, 62 kDa Neisseria meningitidis serogroup … protein
Buffer: 50 mM HEPES, 100 mM NaCl, 0.023% n-Dodecyl β-D-maltoside (DDM), pH: 7
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2018 Nov 26
Conformational flexibility of EptA driven by an interdomain helix provides insights for enzyme-substrate recognition. IUCrJ 8(Pt 5):732-746 (2021)
Anandan A, Dunstan NW, Ryan TM, Mertens HDT, Lim KYL, Evans GL, Kahler CM, Vrielink A
RgGuinier 4.2 nm
Dmax 12.9 nm
VolumePorod 304 nm3

SASDQ85 – Phosphofructokinase B (PfkB) from Mycobacterium marinum

Fructokinase, PfkB experimental SAS data
DAMMIF model
Sample: Fructokinase, PfkB monomer, 32 kDa Mycobacterium marinum (strain … protein
Buffer: 20 mM Tris-HCl, 100 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2019 Dec 17
Structural analysis and functional study of phosphofructokinase B (PfkB) from Mycobacterium marinum Biochemical and Biophysical Research Communications (2021)
Gao B, Ji R, Li Z, Su X, Li H, Sun Y, Ji C, Gan J, Li J
RgGuinier 2.0 nm
Dmax 6.6 nm
VolumePorod 58 nm3

SASDLG9 – Menangle virus (MenV) phosphoprotein, amino acids 209-388 (coiled-coil/flexible linker/binding domain and C352S mutation)

Phosphoprotein experimental SAS data
Phosphoprotein Kratky plot
Sample: Phosphoprotein tetramer, 79 kDa Menangle virus protein
Buffer: 12.5 mM MOPS/KOH pH 7.0, 250 mM NaCl, pH: 7
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2016 Nov 16
Structural Analysis of the Menangle Virus P Protein Reveals a Soft Boundary between Ordered and Disordered Regions Viruses 13(9):1737 (2021)
Webby M, Herr N, Bulloch E, Schmitz M, Keown J, Goldstone D, Kingston R
RgGuinier 6.3 nm
Dmax 23.4 nm
VolumePorod 524 nm3

SASDLH9 – Menangle virus (MenV) phosphoprotein, amino acids 267-388 (flexible linker/binding domain and C352S mutation)

Phosphoprotein experimental SAS data
Phosphoprotein Kratky plot
Sample: Phosphoprotein monomer, 13 kDa Menangle virus protein
Buffer: 12.5 mM MOPS/KOH pH 7.0, 150 mM NaCl, pH: 7
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2016 Aug 17
Structural Analysis of the Menangle Virus P Protein Reveals a Soft Boundary between Ordered and Disordered Regions Viruses 13(9):1737 (2021)
Webby M, Herr N, Bulloch E, Schmitz M, Keown J, Goldstone D, Kingston R
RgGuinier 3.1 nm
Dmax 12.2 nm
VolumePorod 20 nm3

SASDLJ9 – Menangle virus (MenV) phosphoprotein C-terminal fragment, amino acids 267-328 (flexible linker)

Phosphoprotein experimental SAS data
Phosphoprotein Kratky plot
Sample: Phosphoprotein monomer, 6 kDa Menangle virus protein
Buffer: 12.5 mM Tris/HCl pH 8.5, 150 mM NaCl, pH: 8.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Aug 15
Structural Analysis of the Menangle Virus P Protein Reveals a Soft Boundary between Ordered and Disordered Regions Viruses 13(9):1737 (2021)
Webby M, Herr N, Bulloch E, Schmitz M, Keown J, Goldstone D, Kingston R
RgGuinier 2.5 nm
Dmax 10.3 nm
VolumePorod 12 nm3