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SASDKA2 – Two titin I27 domains linked with RS linker

Two titin I27 domains linked with RS linker experimental SAS data
Two titin I27 domains linked with RS linker Rg histogram
Sample: Two titin I27 domains linked with RS linker monomer, 23 kDa protein
Buffer: 50 mM potassium phosphate, 300 mM KCl, DTT 2mM, pH: 7.6
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Dec 4
Interdomain linkers tailor the stability of immunoglobulin repeats in polyproteins. Biochem Biophys Res Commun 550:43-48 (2021)
Joshi T, Garg S, Estaña A, Cortés J, Bernadó P, Das S, Kammath AR, Sagar A, Rakshit S
RgGuinier 3.0 nm

SASDKB2 – Two titin I27 domains linked with RS linker

Two titin I27 domains linked with RS linker experimental SAS data
Sample: Two titin I27 domains linked with RS linker monomer, 23 kDa protein
Buffer: 50 mM potassium phosphate, 300 mM KCl, DTT 2mM, pH: 7.6
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Dec 4
Interdomain linkers tailor the stability of immunoglobulin repeats in polyproteins. Biochem Biophys Res Commun 550:43-48 (2021)
Joshi T, Garg S, Estaña A, Cortés J, Bernadó P, Das S, Kammath AR, Sagar A, Rakshit S
RgGuinier 3.0 nm

SASDKC2 – Two titin I27 domains linked with GGG linker

Two titin I27 domains linked with GGG linker experimental SAS data
Two titin I27 domains linked with GGG linker Rg histogram
Sample: Two titin I27 domains linked with GGG linker monomer, 23 kDa protein
Buffer: 50 mM potassium phosphate, 300 mM KCl, DTT 2mM, pH: 7.6
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Dec 4
Interdomain linkers tailor the stability of immunoglobulin repeats in polyproteins. Biochem Biophys Res Commun 550:43-48 (2021)
Joshi T, Garg S, Estaña A, Cortés J, Bernadó P, Das S, Kammath AR, Sagar A, Rakshit S
RgGuinier 2.9 nm

SASDKD2 – Two titin I27 domains linked with VKA linker

Two titin I27 domains linked with VKA linker experimental SAS data
Two titin I27 domains linked with VKA linker Rg histogram
Sample: Two titin I27 domains linked with VKA linker monomer, 24 kDa protein
Buffer: 50 mM potassium phosphate, 300 mM KCl, DTT 2mM, pH: 7.6
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Dec 4
Interdomain linkers tailor the stability of immunoglobulin repeats in polyproteins. Biochem Biophys Res Commun 550:43-48 (2021)
Joshi T, Garg S, Estaña A, Cortés J, Bernadó P, Das S, Kammath AR, Sagar A, Rakshit S
RgGuinier 3.1 nm

SASDKE2 – Two titin I27 domains linked with AAE linker

Two titin I27 domains linked with AAE linker experimental SAS data
Two titin I27 domains linked with AAE linker Rg histogram
Sample: Two titin I27 domains linked with AAE linker monomer, 24 kDa protein
Buffer: 50 mM potassium phosphate, 300 mM KCl, DTT 2mM, pH: 7.6
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Dec 4
Interdomain linkers tailor the stability of immunoglobulin repeats in polyproteins. Biochem Biophys Res Commun 550:43-48 (2021)
Joshi T, Garg S, Estaña A, Cortés J, Bernadó P, Das S, Kammath AR, Sagar A, Rakshit S
RgGuinier 3.1 nm

SASDKW8 – Full-length pUL21 with C-terminal His-tag

Tegument protein UL21 experimental SAS data
Full-length pUL21 with C-terminal His-tag Rg histogram
Sample: Tegument protein UL21 monomer, 59 kDa Human alphaherpesvirus 1 … protein
Buffer: 20 mM Tris pH 8.5, 500 mM NaCl, 3% (v/v) glycerol, 1 mM DTT, pH: 8.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Sep 25
pUL21 is a viral phosphatase adaptor that promotes herpes simplex virus replication and spread. PLoS Pathog 17(8):e1009824 (2021)
Benedyk TH, Muenzner J, Connor V, Han Y, Brown K, Wijesinghe KJ, Zhuang Y, Colaco S, Stoll GA, Tutt OS, Svobodova S, Svergun DI, Bryant NA, Deane JE, Firth AE, Jeffries CM, Crump CM, Graham SC
RgGuinier 4.2 nm
Dmax 18.1 nm
VolumePorod 90 nm3

SASDKM8 – Rationally optimised WA20 (ROWA) dimer

Rationally optimised WA20 mutant N22A/H86K (ROWA) dimer experimental SAS data
DAMMIN model
Sample: Rationally optimised WA20 mutant N22A/H86K (ROWA) dimer dimer, 25 kDa Artificial protein (de … protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5% glycerol,, pH: 7.5
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2019 Nov 30
Rational thermostabilisation of four-helix bundle dimeric de novo proteins. Sci Rep 11(1):7526 (2021)
Irumagawa S, Kobayashi K, Saito Y, Miyata T, Umetsu M, Kameda T, Arai R
RgGuinier 2.5 nm
Dmax 9.9 nm
VolumePorod 30 nm3

SASDKN8 – Rationally optimised WA20 (ROWA) tetramer

Rationally optimised WA20 mutant N22A/H86K (ROWA) tetramer experimental SAS data
DAMMIN model
Sample: Rationally optimised WA20 mutant N22A/H86K (ROWA) tetramer tetramer, 50 kDa Artificial protein (de … protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5% glycerol,, pH: 7.5
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2019 Nov 30
Rational thermostabilisation of four-helix bundle dimeric de novo proteins. Sci Rep 11(1):7526 (2021)
Irumagawa S, Kobayashi K, Saito Y, Miyata T, Umetsu M, Kameda T, Arai R
RgGuinier 4.1 nm
Dmax 18.1 nm
VolumePorod 60 nm3

SASDKP8 – Rationally optimised WA20 (ROWA) hexamer

Rationally optimised WA20 mutant N22A/H86K (ROWA) hexamer experimental SAS data
DAMMIN model
Sample: Rationally optimised WA20 mutant N22A/H86K (ROWA) hexamer hexamer, 75 kDa Artificial protein (de … protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5% glycerol,, pH: 7.5
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2019 Nov 30
Rational thermostabilisation of four-helix bundle dimeric de novo proteins. Sci Rep 11(1):7526 (2021)
Irumagawa S, Kobayashi K, Saito Y, Miyata T, Umetsu M, Kameda T, Arai R
RgGuinier 5.3 nm
Dmax 24.8 nm
VolumePorod 120 nm3

SASDKQ8 – Rationally optimised SUWA (ROSA) dimer

Rationally optimised SUWA mutant N22E/H86K (ROSA) dimer experimental SAS data
DAMMIN model
Sample: Rationally optimised SUWA mutant N22E/H86K (ROSA) dimer dimer, 25 kDa Artificial protein (de … protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5% glycerol,, pH: 7.5
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2019 Nov 30
Rational thermostabilisation of four-helix bundle dimeric de novo proteins. Sci Rep 11(1):7526 (2021)
Irumagawa S, Kobayashi K, Saito Y, Miyata T, Umetsu M, Kameda T, Arai R
RgGuinier 2.5 nm
Dmax 10.6 nm
VolumePorod 30 nm3