Small Angle Scattering Biological Data Bank SASBDB

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SASDH56 – High Five insect cell culture (control; uninfected)

High Five insect cells experimental SAS data

Sample:	High Five insect cells, 0 kDa Trichoplusia ni
Buffer:	TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Dec 3

Rapid screening of in cellulo grown protein crystals via a small-angle X-ray scattering/X-ray powder diffraction synergistic approach Journal of Applied Crystallography 53(5) (2020)
Lahey-Rudolph J, Schönherr R, Jeffries C, Blanchet C, Boger J, Ferreira Ramos A, Riekehr W, Triandafillidis D, Valmas A, Margiolaki I, Svergun D, Redecke L

SASDH66 – In cellulo Woronin body major protein (HEX-1) crystals recombinantly expressed within High Five insect cells (cell-culture serial dilution series)

Woronin body major protein experimental SAS data

Sample:	Woronin body major protein, 19 kDa Neurospora crassa protein
Buffer:	TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Dec 3

SASDH76 – In cellulo cathepsin B (CatB) protein crystals recombinantly expressed within High Five insect cells (cell-culture serial dilution series)

Cathepsin B-like cysteine protease experimental SAS data

Cathepsin B-like cysteine protease Kratky plot

Sample:	Cathepsin B-like cysteine protease, 37 kDa Trypanosoma brucei protein
Buffer:	TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Dec 3

SASDH86 – In cellulo cathepsin B (CatB) protein crystals recombinantly expressed within High Five insect cells

Sample:	Cathepsin B-like cysteine protease, 37 kDa Trypanosoma brucei protein
Buffer:	TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Nov 21

SASDH96 – In cellulo cathepsin B (CatB) protein crystals recombinantly expressed within Sf9 insect cells

Sample:	Cathepsin B-like cysteine protease, 37 kDa Trypanosoma brucei protein
Buffer:	TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Nov 21

SASDHA6 – In cellulo Woronin body major protein (HEX-1) crystals recombinantly expressed within High Five insect cells

Sample:	Woronin body major protein, 19 kDa Neurospora crassa protein
Buffer:	TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Nov 21

SASDHB6 – In cellulo Woronin body major protein (HEX-1) crystals recombinantly expressed within Sf9 insect cells

Sample:	Woronin body major protein, 19 kDa Neurospora crassa protein
Buffer:	TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Nov 21

SASDJY5 – In cellulo inosine-5'-monophosphate dehydrogenase (IMPDH) protein crystals recombinantly expressed within High Five insect cells

Inosine-5'-monophosphate dehydrogenase experimental SAS data

Inosine-5'-monophosphate dehydrogenase Kratky plot

Sample:	Inosine-5'-monophosphate dehydrogenase, 59 kDa Trypanosoma brucei brucei protein
Buffer:	20 mM Tris, 150 mM NaCl,, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Nov 21

SASDJZ5 – In cellulo inosine-5'-monophosphate dehydrogenase (IMPDH) protein crystals recombinantly expressed within Sf9 insect cells

Sample:	Inosine-5'-monophosphate dehydrogenase, 59 kDa Trypanosoma brucei brucei protein
Buffer:	20 mM Tris, 150 mM NaCl,, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Nov 21

SASDHP5 – Rov C - Regulator Of Virulence interconnected with the Csr system (hexameric transcriptional activator of type VI secretion gene expression)

Regulator Of Virulence interconnected with the Csr system experimental SAS data

Sample:	Regulator Of Virulence interconnected with the Csr system hexamer, 175 kDa Yersinia pseudotuberculosis protein
Buffer:	50 mM TRIS pH= 8, 500 mM NaCl, 5 mM DTT, 5 % v/v glycerol, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Jun 18

RovC - a novel type of hexameric transcriptional activator promoting type VI secretion gene expression PLOS Pathogens 16(9):e1008552 (2020)
Knittel V, Sadana P, Seekircher S, Stolle A, Körner B, Volk M, Jeffries C, Svergun D, Heroven A, Scrima A, Dersch P, Mecsas J

R_g^Guinier	4.2	nm
D_max	12.4	nm
Volume^Porod	280	nm³

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