|
|
|
|
|
| Sample: |
Tegument protein UL21 monomer, 59 kDa Human alphaherpesvirus 1 … protein
|
| Buffer: |
20 mM Tris pH 8.5, 500 mM NaCl, 3% (v/v) glycerol, 1 mM DTT, pH: 8.5 |
| Experiment: |
SAXS
data collected at EMBL P12, PETRA III on 2020 Sep 25
|
pUL21 is a viral phosphatase adaptor that promotes herpes simplex virus replication and spread.
PLoS Pathog 17(8):e1009824 (2021)
Benedyk TH, Muenzner J, Connor V, Han Y, Brown K, Wijesinghe KJ, Zhuang Y, Colaco S, Stoll GA, Tutt OS, Svobodova S, Svergun DI, Bryant NA, Deane JE, Firth AE, Jeffries CM, Crump CM, Graham SC
|
| RgGuinier |
4.2 |
nm |
| Dmax |
18.1 |
nm |
| VolumePorod |
90 |
nm3 |
|
|
|
|
|
|
|
| Sample: |
Rationally optimised WA20 mutant N22A/H86K (ROWA) dimer dimer, 25 kDa Artificial protein (de … protein
|
| Buffer: |
20 mM HEPES, 150 mM NaCl, 5% glycerol,, pH: 7.5 |
| Experiment: |
SAXS
data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2019 Nov 30
|
Rational thermostabilisation of four-helix bundle dimeric de novo proteins.
Sci Rep 11(1):7526 (2021)
Irumagawa S, Kobayashi K, Saito Y, Miyata T, Umetsu M, Kameda T, Arai R
|
| RgGuinier |
2.5 |
nm |
| Dmax |
9.9 |
nm |
| VolumePorod |
30 |
nm3 |
|
|
|
|
|
|
|
| Sample: |
Rationally optimised WA20 mutant N22A/H86K (ROWA) tetramer tetramer, 50 kDa Artificial protein (de … protein
|
| Buffer: |
20 mM HEPES, 150 mM NaCl, 5% glycerol,, pH: 7.5 |
| Experiment: |
SAXS
data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2019 Nov 30
|
Rational thermostabilisation of four-helix bundle dimeric de novo proteins.
Sci Rep 11(1):7526 (2021)
Irumagawa S, Kobayashi K, Saito Y, Miyata T, Umetsu M, Kameda T, Arai R
|
| RgGuinier |
4.1 |
nm |
| Dmax |
18.1 |
nm |
| VolumePorod |
60 |
nm3 |
|
|
|
|
|
|
|
| Sample: |
Rationally optimised WA20 mutant N22A/H86K (ROWA) hexamer hexamer, 75 kDa Artificial protein (de … protein
|
| Buffer: |
20 mM HEPES, 150 mM NaCl, 5% glycerol,, pH: 7.5 |
| Experiment: |
SAXS
data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2019 Nov 30
|
Rational thermostabilisation of four-helix bundle dimeric de novo proteins.
Sci Rep 11(1):7526 (2021)
Irumagawa S, Kobayashi K, Saito Y, Miyata T, Umetsu M, Kameda T, Arai R
|
| RgGuinier |
5.3 |
nm |
| Dmax |
24.8 |
nm |
| VolumePorod |
120 |
nm3 |
|
|
|
|
|
|
|
| Sample: |
Rationally optimised SUWA mutant N22E/H86K (ROSA) dimer dimer, 25 kDa Artificial protein (de … protein
|
| Buffer: |
20 mM HEPES, 150 mM NaCl, 5% glycerol,, pH: 7.5 |
| Experiment: |
SAXS
data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2019 Nov 30
|
Rational thermostabilisation of four-helix bundle dimeric de novo proteins.
Sci Rep 11(1):7526 (2021)
Irumagawa S, Kobayashi K, Saito Y, Miyata T, Umetsu M, Kameda T, Arai R
|
| RgGuinier |
2.5 |
nm |
| Dmax |
10.6 |
nm |
| VolumePorod |
30 |
nm3 |
|
|
|
|
|
|
|
| Sample: |
Rationally optimised SUWA mutant N22E/H86K (ROSA) tetramer tetramer, 50 kDa Artificial protein (de … protein
|
| Buffer: |
20 mM HEPES, 150 mM NaCl, 5% glycerol,, pH: 7.5 |
| Experiment: |
SAXS
data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2019 Nov 30
|
Rational thermostabilisation of four-helix bundle dimeric de novo proteins.
Sci Rep 11(1):7526 (2021)
Irumagawa S, Kobayashi K, Saito Y, Miyata T, Umetsu M, Kameda T, Arai R
|
| RgGuinier |
4.4 |
nm |
| Dmax |
22.1 |
nm |
| VolumePorod |
88 |
nm3 |
|
|
|
|
|
|
|
| Sample: |
Mixed-sequence 25 base-paired DNA dimer, 16 kDa DNA
|
| Buffer: |
1.0 mM MgCl2, 10 mM Na-MOPS and 20uM EDTA, pH: 7 |
| Experiment: |
SAXS
data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
|
The structural plasticity of nucleic acid duplexes revealed by WAXS and MD.
Sci Adv 7(17) (2021)
He W, Chen YL, Pollack L, Kirmizialtin S
|
| RgGuinier |
2.0 |
nm |
| Dmax |
7.8 |
nm |
| VolumePorod |
17 |
nm3 |
|
|
|
|
|
|
|
| Sample: |
AT-sequence 25 base-paired DNA dimer, 16 kDa DNA
|
| Buffer: |
1.0 mM MgCl2, 10 mM Na-MOPS and 20uM EDTA, pH: 7 |
| Experiment: |
SAXS
data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
|
The structural plasticity of nucleic acid duplexes revealed by WAXS and MD.
Sci Adv 7(17) (2021)
He W, Chen YL, Pollack L, Kirmizialtin S
|
| RgGuinier |
2.1 |
nm |
| Dmax |
8.0 |
nm |
| VolumePorod |
16 |
nm3 |
|
|
|
|
|
|
|
| Sample: |
Wild type dENE monomer, 23 kDa RNA
|
| Buffer: |
50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7 |
| Experiment: |
SAXS
data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
|
Structural analyses of an RNA stability element interacting with poly(A)
Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
Torabi S, Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
|
| RgGuinier |
2.8 |
nm |
| Dmax |
10.8 |
nm |
| VolumePorod |
31 |
nm3 |
|
|
|
|
|
|
|
| Sample: |
Wild type dENE + Poly(A)20 monomer, 30 kDa RNA
|
| Buffer: |
50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7 |
| Experiment: |
SAXS
data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
|
Structural analyses of an RNA stability element interacting with poly(A)
Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
Torabi S, Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
|
| RgGuinier |
2.8 |
nm |
| Dmax |
10.7 |
nm |
|
|
