Small Angle Scattering Biological Data Bank SASBDB

Browse by ORGANISM: other species

SASDG27 – Salt stress-induced protein at a sample concentration of 22mg/ml

Salt stress-induced protein experimental SAS data

Sample:	Salt stress-induced protein dimer, 37 kDa Oryza sativa Indica … protein
Buffer:	50 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH: 7.4
Experiment:	SAXS data collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) on 2017 Mar 6

Structural insights into rice SalTol QTL located SALT protein. Sci Rep 10(1):16589 (2020)
Kaur N, Sagar A, Sharma P, Ashish, Pati PK

R_g^Guinier	2.5	nm
D_max	6.5	nm
Volume^Porod	57	nm³

SASDJH3 – Hen Egg White Lysozyme in presence of 100 mM 5-methyl uridine

Sample:	Lysozyme C monomer, 16 kDa Gallus gallus protein
Buffer:	40 mM sodium acetate, 150 mM NaCl, 100 mM 5-methyl uridine, pH: 3.8
Experiment:	SAXS data collected at BL11 - NCD, ALBA on 2019 Jun 7

Improving data quality and expanding BioSAXS experiments to low-molecular-weight and low-concentration protein samples. Acta Crystallogr D Struct Biol 76(Pt 10):971-981 (2020)
Castellví A, Pascual-Izarra C, Crosas E, Malfois M, Juanhuix J

R_g^Guinier	1.5	nm
D_max	4.2	nm
Volume^Porod	21	nm³

SASDJJ3 – SH3 domain of alpha spectrin (amino acids 965-1025) in presence of 100 mM 5-methyl uridine

Spectrin alpha chain, non-erythrocytic 1 experimental SAS data

Spectrin alpha chain, non-erythrocytic 1 Kratky plot

Sample:	Spectrin alpha chain, non-erythrocytic 1, 7 kDa Gallus gallus protein
Buffer:	10 mM citric acid, 150 mM NaCl, 100 mM 5-methyl uridine, pH: 3
Experiment:	SAXS data collected at BL11 - NCD, ALBA on 2019 Jun 14

R_g^Guinier	1.3	nm
D_max	4.5	nm
Volume^Porod	10	nm³

SASDHR5 – Heat shock protein 40, subfamily A (PfHSP40)

HSP40, subfamily A experimental SAS data

Sample:	HSP40, subfamily A dimer, 97 kDa Plasmodium falciparum protein
Buffer:	50 mM Tris, 5 mM MgCl2, 300 mM KCl, 1 mM β-mercaptoethanol, 5% glycerol, pH: 8
Experiment:	SAXS data collected at Anton Paar SAXSpace, CSIR-Central Drug Research Institute on 2019 Oct 24

Structural-functional diversity of malaria parasite's PfHSP70-1 and PfHSP40 chaperone pair gives an edge over human orthologs in chaperone-assisted protein folding. Biochem J 477(18):3625-3643 (2020)
Anas M, Shukla A, Tripathi A, Kumari V, Prakash C, Nag P, Kumar LS, Sharma SK, Ramachandran R, Kumar N

R_g^Guinier	4.3	nm
D_max	15.0	nm
Volume^Porod	339	nm³

SASDKJ4 – SARS-CoV-2 nsp7 and nsp8

Replicase polyprotein 1a - nsp7Replicase polyprotein 1a - nsp8 experimental SAS data

Sample:	Replicase polyprotein 1a - nsp7, 18 kDa Severe acute respiratory … protein Replicase polyprotein 1a - nsp8, 44 kDa Severe acute respiratory … protein
Buffer:	50 mM Tris, pH 8.0, 100 mM NaCl, 4 mM DTT, 4 mM MgCl2, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Jun 19

Hallmarks of Alpha- and Betacoronavirus non-structural protein 7+8 complexes Science Advances 7(10):eabf1004 (2021)
Krichel B, Bylapudi G, Schmidt C, Blanchet C, Schubert R, Brings L, Koehler M, Zenobi R, Svergun D, Lorenzen K, Madhugiri R, Ziebuhr J, Uetrecht C

R_g^Guinier	3.4	nm
D_max	13.5	nm
Volume^Porod	92	nm³

SASDHY5 – In cellulo luciferase protein crystals recombinantly expressed within High Five insect cells

Photinus pyralis firefly luciferase experimental SAS data

Photinus pyralis firefly luciferase Kratky plot

Sample:	Photinus pyralis firefly luciferase, 61 kDa Photinus pyralis protein
Buffer:	TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Dec 3

Rapid screening of in cellulo grown protein crystals via a small-angle X-ray scattering/X-ray powder diffraction synergistic approach Journal of Applied Crystallography 53(5) (2020)
Lahey-Rudolph J, Schönherr R, Jeffries C, Blanchet C, Boger J, Ferreira Ramos A, Riekehr W, Triandafillidis D, Valmas A, Margiolaki I, Svergun D, Redecke L

SASDHZ5 – In cellulo inosine-5'-monophosphate dehydrogenase (IMPDH) protein crystals recombinantly expressed within High Five insect cells

Inosine-5'-monophosphate dehydrogenase experimental SAS data

Inosine-5'-monophosphate dehydrogenase Kratky plot

Sample:	Inosine-5'-monophosphate dehydrogenase, 59 kDa Trypanosoma brucei brucei protein
Buffer:	TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Dec 3

SASDH26 – In cellulo cathepsin B (CatB) protein crystals recombinantly expressed within High Five insect cells

Cathepsin B-like cysteine protease experimental SAS data

Cathepsin B-like cysteine protease Kratky plot

Sample:	Cathepsin B-like cysteine protease, 37 kDa Trypanosoma brucei protein
Buffer:	TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Dec 3

SASDH36 – In cellulo Woronin body major protein (HEX-1) crystals recombinantly expressed within High Five insect cells

Woronin body major protein experimental SAS data

Sample:	Woronin body major protein, 19 kDa Neurospora crassa protein
Buffer:	TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Dec 3

SASDH46 – High Five insect cell culture infected with mock recombinant baculovirus (rBV)

mock recombinant baculovirus experimental SAS data

mock recombinant baculovirus Kratky plot

Sample:	Mock recombinant baculovirus, 0 kDa unidentified baculovirus
Buffer:	TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Dec 3

First << Prev Page 127 of 207 Next >> Last