Browse by ORGANISM: other species

SASDKA3 – double Element for Nuclear Expression (dENE) Crystal Structure + Poly(A)25

Xtal dENE + Poly(A)25 experimental SAS data
Xtal dENE + Poly(A)25 Kratky plot
Sample: Xtal dENE + Poly(A)25 monomer, 36 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2019 Dec 5
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
Torabi S, Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.3 nm
Dmax 12.4 nm

SASDKB3 – double Element for Nuclear Expression (dENE) Crystal Structure + Poly(A)30

Xtal dENE + Poly(A)30 experimental SAS data
Xtal dENE + Poly(A)30 Kratky plot
Sample: Xtal dENE + Poly(A)30 monomer, 38 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2019 Dec 5
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
Torabi S, Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.5 nm
Dmax 13.2 nm

SASDKC3 – double Element for Nuclear Expression (dENE) Mutant A + Poly(A)25

A dENE + Poly(A)25 experimental SAS data
A dENE + Poly(A)25 Kratky plot
Sample: A dENE + Poly(A)25 monomer, 36 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
Torabi S, Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.3 nm
Dmax 12.4 nm

SASDKD3 – double Element for Nuclear Expression (dENE) Mutant B + Hairpin - Poly(A)30

B dENE + Hairpin - Poly(A)30 experimental SAS data
B dENE + Hairpin - Poly(A)30 Kratky plot
Sample: B dENE + Hairpin - Poly(A)30 monomer, 39 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
Torabi S, Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.5 nm
Dmax 14.0 nm

SASDKE3 – double Element for Nuclear Expression (dENE) Crystal Structure + Poly(A)20

Xtal dENE + Poly(A)20 experimental SAS data
Xtal dENE + Poly(A)20 Kratky plot
Sample: Xtal dENE + Poly(A)20 monomer, 34 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2019 Dec 5
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
Torabi S, Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.2 nm
Dmax 11.8 nm

SASDXG3 – CP12 protein from the marine diatom Thalassiosira pseudonana

CP12 domain-containing protein experimental SAS data
GASBOR model
Sample: CP12 domain-containing protein dimer, 34 kDa Thalassiosira pseudonana protein
Buffer: 30 mM Tris, 50 mM NaCl, 2 mM EDTA, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Feb 23
A new type of flexible CP12 protein in the marine diatom Thalassiosira pseudonana Cell Communication and Signaling 19(1) (2021)
Shao H, Huang W, Avilan L, Receveur-Bréchot V, Puppo C, Puppo R, Lebrun R, Gontero B, Launay H
RgGuinier 3.8 nm
Dmax 13.5 nm

SASDKZ4 – the Gemin2/Survival Motor Neuron-like protein 1 (SMN1) complex from Schizosaccharomyces pombe

Survival motor neuron-like protein 1Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) experimental SAS data
Survival motor neuron-like protein 1 Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) Kratky plot
Sample: Survival motor neuron-like protein 1 monomer, 17 kDa Schizosaccharomyces pombe (strain … protein
Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) monomer, 18 kDa Schizosaccharomyces pombe (strain … protein
Buffer: 150 mM NaCl, 20 mM HEPES, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 29
Identification and structural analysis of the Schizosaccharomyces pombe SMN complex Nucleic Acids Research (2021)
Veepaschit J, Viswanathan A, Bordonné R, Grimm C, Fischer U
RgGuinier 8.4 nm
Dmax 29.0 nm

SASDK25 – the Gemin2/Survival Motor Neuron-like protein 1 (SMN1) complex from Schizosaccharomyces pombe, 0.165 g/L

Survival motor neuron-like protein 1Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) experimental SAS data
Survival motor neuron-like protein 1 Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) Kratky plot
Sample: Survival motor neuron-like protein 1 monomer, 17 kDa Schizosaccharomyces pombe (strain … protein
Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) monomer, 18 kDa Schizosaccharomyces pombe (strain … protein
Buffer: 150 mM NaCl, 20 mM HEPES, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 29
Identification and structural analysis of the Schizosaccharomyces pombe SMN complex Nucleic Acids Research (2021)
Veepaschit J, Viswanathan A, Bordonné R, Grimm C, Fischer U
RgGuinier 8.3 nm
Dmax 32.0 nm

SASDK35 – the Gemin2/Survival Motor Neuron-like protein 1 (SMN1) complex from Schizosaccharomyces pombe, 0.419 g/L

Survival motor neuron-like protein 1Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) experimental SAS data
Survival motor neuron-like protein 1 Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) Kratky plot
Sample: Survival motor neuron-like protein 1 monomer, 17 kDa Schizosaccharomyces pombe (strain … protein
Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) monomer, 18 kDa Schizosaccharomyces pombe (strain … protein
Buffer: 150 mM NaCl, 20 mM HEPES, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 29
Identification and structural analysis of the Schizosaccharomyces pombe SMN complex Nucleic Acids Research (2021)
Veepaschit J, Viswanathan A, Bordonné R, Grimm C, Fischer U
RgGuinier 8.2 nm
Dmax 29.7 nm

SASDK45 – the Gemin2/Survival Motor Neuron-like protein 1 (SMN1) complex from Schizosaccharomyces pombe, 0.879 g/L

Survival motor neuron-like protein 1Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) experimental SAS data
Survival motor neuron-like protein 1 Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) Kratky plot
Sample: Survival motor neuron-like protein 1 monomer, 17 kDa Schizosaccharomyces pombe (strain … protein
Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) monomer, 18 kDa Schizosaccharomyces pombe (strain … protein
Buffer: 150 mM NaCl, 20 mM HEPES, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 29
Identification and structural analysis of the Schizosaccharomyces pombe SMN complex Nucleic Acids Research (2021)
Veepaschit J, Viswanathan A, Bordonné R, Grimm C, Fischer U
RgGuinier 8.1 nm
Dmax 29.0 nm