Browse by ORGANISM: other species

SASDJT3 – Adenylate cyclase toxin CyaA RTX block III-V

Bifunctional hemolysin/adenylate cyclase experimental SAS data
DAMMIF model
Sample: Bifunctional hemolysin/adenylate cyclase monomer, 45 kDa Bordetella pertussis protein
Buffer: 10 mM Tris HCl, 150 mM NaCl, 10 mM CaCl₂, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Oct 31
Continuous Assembly of β-Roll Structures Is Implicated in the Type I-Dependent Secretion of Large Repeat-in-Toxins (RTX) Proteins. J Mol Biol (2020)
Motlova L, Klimova N, Fiser R, Sebo P, Bumba L
RgGuinier 3.4 nm
Dmax 12.1 nm
VolumePorod 85 nm3

SASDJU3 – Adenylate cyclase toxin CyaA RTX block IV-V

Bifunctional hemolysin/adenylate cyclase experimental SAS data
DAMMIF model
Sample: Bifunctional hemolysin/adenylate cyclase monomer, 32 kDa Bordetella pertussis protein
Buffer: 10 mM Tris HCl, 150 mM NaCl, 10 mM CaCl₂, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Oct 31
Continuous Assembly of β-Roll Structures Is Implicated in the Type I-Dependent Secretion of Large Repeat-in-Toxins (RTX) Proteins. J Mol Biol (2020)
Motlova L, Klimova N, Fiser R, Sebo P, Bumba L
RgGuinier 2.7 nm
Dmax 8.6 nm
VolumePorod 45 nm3

SASDJB4 – Endonuclease VIII - Like 2 from Monodelphis domestica (size exclusion chromatography SAXS) full length

Nei like DNA glycosylase 2 experimental SAS data
Nei like DNA glycosylase 2 Kratky plot
Sample: Nei like DNA glycosylase 2 monomer, 39 kDa Monodelphis domestica protein
Buffer: 25 mM Bis-Tris, 150 mM NaCl, 2% glycerol, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Mar 9
Unique Structural Features of Mammalian NEIL2 DNA Glycosylase Prime Its Activity for Diverse DNA Substrates and Environments. Structure (2020)
Eckenroth BE, Cao VB, Averill AM, Dragon JA, Doublié S
RgGuinier 2.7 nm
Dmax 8.2 nm
VolumePorod 58 nm3

SASDJC4 – Endonuclease VIII - Like 2 from Monodelphis domestica (size exclusion chromatography SAXS) MdoNEIL2 cut (Δ67-133)

Nei like DNA glycosylase 2 (Δ67-133) experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Nei like DNA glycosylase 2 (Δ67-133) monomer, 33 kDa Monodelphis domestica protein
Buffer: 25 mM Bis-Tris, 150 mM NaCl, 2% glycerol, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Mar 9
Unique Structural Features of Mammalian NEIL2 DNA Glycosylase Prime Its Activity for Diverse DNA Substrates and Environments. Structure (2020)
Eckenroth BE, Cao VB, Averill AM, Dragon JA, Doublié S
RgGuinier 2.4 nm
Dmax 7.8 nm
VolumePorod 47 nm3

SASDHU9 – Solution structure of middle domain of the multi-domain GH18 chitinase ChiA from Flavobacterium johnsoniae

Chitinase ChiA experimental SAS data
DAMMIN model
Sample: Chitinase ChiA monomer, 70 kDa Flavobacterium johnsoniae protein
Buffer: 50 mM Tris, 250 mM NaCl, 0.25 mM DTT, pH: 8
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2019 May 7
Structural insights of the enzymes from the chitin utilization locus of Flavobacterium johnsoniae. Sci Rep 10(1):13775 (2020)
Mazurkewich S, Helland R, Mackenzie A, Eijsink VGH, Pope PB, Brändén G, Larsbrink J
RgGuinier 4.7 nm
Dmax 16.3 nm
VolumePorod 99 nm3

SASDJ63 – Streptococcus pneumoniae NADPH oxidase - Tag-free SpNOX

FAD-binding FR-type domain-containing protein experimental SAS data
OTHER model
Sample: FAD-binding FR-type domain-containing protein monomer, 47 kDa Streptococcus pneumoniae protein
Buffer: 50 mM Tris-HCl pH 7, 300 mM NaCl, 5 mM LMNG, 10 µM FAD, 21.4% D₂O, pH: 7
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2018 Jun 26
Interdomain Flexibility within NADPH Oxidase Suggested by SANS Using LMNG Stealth Carrier. Biophys J 119(3):605-618 (2020)
Vermot A, Petit-Härtlein I, Breyton C, Le Roy A, Thépaut M, Vivès C, Moulin M, Härtlein M, Grudinin S, Smith SME, Ebel C, Martel A, Fieschi F
RgGuinier 3.0 nm
Dmax 10.0 nm
VolumePorod 89 nm3

SASDHJ4 – Dengue Virus 2 New Guinea C (DENV-2 C, capsid protein), apo form

Dengue Virus 2 New Guinea C experimental SAS data
CORAL model
Sample: Dengue Virus 2 New Guinea C dimer, 19 kDa Dengue virus 2 protein
Buffer: 100 mM NaCl, 25 mM HEPES, pH: 7.4
Experiment: SAXS data collected at Rigaku BioSAXS-1000, Sealy Center For Structural Biology, UTMB-G on 2018 Jul 5
A cocrystal structure of dengue capsid protein in complex of inhibitor. Proc Natl Acad Sci U S A 117(30):17992-18001 (2020)
Xia H, Xie X, Zou J, Noble CG, Russell WK, Holthauzen LMF, Choi KH, White MA, Shi PY
RgGuinier 1.8 nm
Dmax 5.4 nm
VolumePorod 52 nm3

SASDHK4 – Dengue Virus 2 New Guinea C (DENV-2 C, capsid protein) with saturating ST148 inhibitor

Dengue Virus 2 New Guinea C experimental SAS data
CORAL model
Sample: Dengue Virus 2 New Guinea C dimer, 19 kDa Dengue virus 2 protein
Buffer: 100 mM NaCl, 25 mM HEPES, pH 7.4, 10 µM ST148, pH: 7.4
Experiment: SAXS data collected at Rigaku BioSAXS-1000, Sealy Center For Structural Biology, UTMB-G on 2018 Jul 26
A cocrystal structure of dengue capsid protein in complex of inhibitor. Proc Natl Acad Sci U S A 117(30):17992-18001 (2020)
Xia H, Xie X, Zou J, Noble CG, Russell WK, Holthauzen LMF, Choi KH, White MA, Shi PY
RgGuinier 1.9 nm
Dmax 6.0 nm
VolumePorod 52 nm3

SASDHR8 – Mosquito-larvicidal binary toxin receptor (Cqm1) protein

Synthetic construct (mutant, His-tagged): Mosquito-larvicidal BinAB toxin receptor protein (Neutral and basic amino acid transport protein rBAT) experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Synthetic construct (mutant, His-tagged): Mosquito-larvicidal BinAB toxin receptor protein (Neutral and basic amino acid transport protein rBAT) dimer, 129 kDa Culex quinquefasciatus protein
Buffer: PBS buffer (10 mM Na2HPO4, 1.8 mM KH2PO4, 137 mM NaCl, 2.7 mM KCl), pH: 7.4
Experiment: SAXS data collected at BL-18, INDUS-2 on 2020 Feb 24
Liposome-Based Study Provides Insight into Cellular Internalization Mechanism of Mosquito-Larvicidal BinAB Toxin. J Membr Biol (2020)
Sharma M, Kumar A, Kumar V
RgGuinier 4.0 nm
Dmax 9.2 nm
VolumePorod 97 nm3

SASDHS8 – Mosquito-larvicidal BinB protein

Binary larvicide subunit BinB experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Binary larvicide subunit BinB monomer, 53 kDa Lysinibacillus sphaericus protein
Buffer: PBS buffer (10 mM Na2HPO4, 1.8 mM KH2PO4, 137 mM NaCl, 2.7 mM KCl), pH: 7.4
Experiment: SAXS data collected at BL-18, INDUS-2 on 2020 Feb 24
Liposome-Based Study Provides Insight into Cellular Internalization Mechanism of Mosquito-Larvicidal BinAB Toxin. J Membr Biol (2020)
Sharma M, Kumar A, Kumar V
RgGuinier 2.9 nm
Dmax 9.7 nm
VolumePorod 48 nm3