Browse by ORGANISM: other species

SASDHT8 – Complex of mosquito-larvicidal BinB protein and the Mosquito-larvicidal binary toxin receptor (Cqm1)

Binary larvicide subunit BinBSynthetic construct (mutant, His-tagged): Mosquito-larvicidal BinAB toxin receptor protein (Neutral and basic amino acid transport protein rBAT) experimental SAS data
OTHER model
Sample: Binary larvicide subunit BinB monomer, 53 kDa Lysinibacillus sphaericus protein
Synthetic construct (mutant, His-tagged): Mosquito-larvicidal BinAB toxin receptor protein (Neutral and basic amino acid transport protein rBAT) monomer, 65 kDa Culex quinquefasciatus protein
Buffer: PBS buffer (10 mM Na2HPO4, 1.8 mM KH2PO4, 137 mM NaCl, 2.7 mM KCl), pH: 7.4
Experiment: SAXS data collected at BL-18, INDUS-2 on 2020 Feb 24
Liposome-Based Study Provides Insight into Cellular Internalization Mechanism of Mosquito-Larvicidal BinAB Toxin. J Membr Biol (2020)
Sharma M, Kumar A, Kumar V
RgGuinier 4.6 nm
Dmax 11.7 nm
VolumePorod 290 nm3

SASDHU8 – SANS study of the complex between mosquito-larvicidal BinB protein and the mosquito-larvicidal binary toxin receptor (Cqm1)

Binary larvicide subunit BinBSynthetic construct (mutant, His-tagged): Mosquito-larvicidal BinAB toxin receptor protein (Neutral and basic amino acid transport protein rBAT) experimental SAS data
OTHER model
Sample: Binary larvicide subunit BinB monomer, 53 kDa Lysinibacillus sphaericus protein
Synthetic construct (mutant, His-tagged): Mosquito-larvicidal BinAB toxin receptor protein (Neutral and basic amino acid transport protein rBAT) monomer, 65 kDa Culex quinquefasciatus protein
Buffer: 25 mM HEPES, pH 7.5, 25 mM NaCl, in 100% D2O, pH: 7.5
Experiment: SANS data collected at SANS-I facility, Dhruva Reactor, Bhabha Atomic Research Centre on 2019 Apr 26
Liposome-Based Study Provides Insight into Cellular Internalization Mechanism of Mosquito-Larvicidal BinAB Toxin. J Membr Biol (2020)
Sharma M, Kumar A, Kumar V
RgGuinier 3.7 nm
Dmax 12.9 nm
VolumePorod 174 nm3

SASDJM2 – Flagellotropic bacteriophage YSD1 protein, YSD1_22 - Major Tail Protein

Major tail protein experimental SAS data
DAMFILT model
Sample: Major tail protein monomer, 40 kDa Salmonella virus Chi protein
Buffer: 20 mM Tris, 150 mM NaCl, 0.03 % NaN3, 5.0 % glycerol, pH: 7.8
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Mar 8
The architecture and stabilisation of flagellotropic tailed bacteriophages. Nat Commun 11(1):3748 (2020)
Hardy JM, Dunstan RA, Grinter R, Belousoff MJ, Wang J, Pickard D, Venugopal H, Dougan G, Lithgow T, Coulibaly F
RgGuinier 3.3 nm
Dmax 10.2 nm
VolumePorod 54 nm3

SASDH38 – Wildtype preQ1 riboswitch in Bacillus subtilis, apo-state - Bsu-WT-apo

wildtype preQ1 riboswitch in Bacillus subtilis experimental SAS data
wildtype preQ1 riboswitch in Bacillus subtilis Kratky plot
Sample: Wildtype preQ1 riboswitch in Bacillus subtilis monomer, 11 kDa Bacillus subtilis RNA
Buffer: 50 mM potassium phosphate, 2 mM MgCl2, 50 mM KCl, pH: 6.5
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2018 Mar 23
Hierarchical Conformational Dynamics Confers Thermal Adaptability to preQ1 RNA Riboswitches. J Mol Biol 432(16):4523-4543 (2020)
Gong Z, Yang S, Dong X, Yang QF, Zhu YL, Xiao Y, Tang C
RgGuinier 2.6 nm
Dmax 10.5 nm
VolumePorod 20 nm3

SASDH48 – Wildtype preQ1 riboswitch in Bacillus subtilis, holo-state - Bsu-WT-holo

wildtype preQ1 riboswitch in Bacillus subtilis experimental SAS data
wildtype preQ1 riboswitch in Bacillus subtilis Kratky plot
Sample: Wildtype preQ1 riboswitch in Bacillus subtilis monomer, 11 kDa Bacillus subtilis RNA
Buffer: 50 mM potassium phosphate, 2 mM MgCl2, 50 mM KCl, pH: 6.5
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2018 Mar 23
Hierarchical Conformational Dynamics Confers Thermal Adaptability to preQ1 RNA Riboswitches. J Mol Biol 432(16):4523-4543 (2020)
Gong Z, Yang S, Dong X, Yang QF, Zhu YL, Xiao Y, Tang C
RgGuinier 2.0 nm
Dmax 9.0 nm
VolumePorod 16 nm3

SASDH58 – C15 deletion preQ1 riboswitch in Bacillus subtilis, apo-state - Bsu-C15d-apo

C15 deletion preQ1 riboswitch in Bacillus subtilis experimental SAS data
C15 deletion preQ1 riboswitch in Bacillus subtilis Kratky plot
Sample: C15 deletion preQ1 riboswitch in Bacillus subtilis monomer, 11 kDa Bacillus subtilis RNA
Buffer: 50 mM potassium phosphate, 2 mM MgCl2, 50 mM KCl, pH: 6.5
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2018 Mar 23
Hierarchical Conformational Dynamics Confers Thermal Adaptability to preQ1 RNA Riboswitches. J Mol Biol 432(16):4523-4543 (2020)
Gong Z, Yang S, Dong X, Yang QF, Zhu YL, Xiao Y, Tang C
RgGuinier 1.9 nm
Dmax 8.0 nm
VolumePorod 17 nm3

SASDH68 – C15 deletion preQ1 riboswitch in Bacillus subtilis, holo-state - Bsu-C15d-holo

C15 deletion preQ1 riboswitch in Bacillus subtilis experimental SAS data
C15 deletion preQ1 riboswitch in Bacillus subtilis Kratky plot
Sample: C15 deletion preQ1 riboswitch in Bacillus subtilis monomer, 11 kDa Bacillus subtilis RNA
Buffer: 50 mM potassium phosphate, 2 mM MgCl2, 50 mM KCl, pH: 6.5
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2018 Mar 23
Hierarchical Conformational Dynamics Confers Thermal Adaptability to preQ1 RNA Riboswitches. J Mol Biol 432(16):4523-4543 (2020)
Gong Z, Yang S, Dong X, Yang QF, Zhu YL, Xiao Y, Tang C
RgGuinier 2.0 nm
Dmax 8.8 nm
VolumePorod 14 nm3

SASDH78 – Wildtype preQ1 riboswitch in Thermoanaerobacter tengcongensis, apo-state - Tte-WT-apo

wildtype preQ1 riboswitch in Thermoanaerobacter tengcongensis experimental SAS data
wildtype preQ1 riboswitch in Thermoanaerobacter tengcongensis Kratky plot
Sample: Wildtype preQ1 riboswitch in Thermoanaerobacter tengcongensis monomer, 11 kDa Caldanaerobacter subterraneus subsp. … RNA
Buffer: 50 mM potassium phosphate, 2 mM MgCl2, 50 mM KCl, pH: 6.5
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2018 Mar 23
Hierarchical Conformational Dynamics Confers Thermal Adaptability to preQ1 RNA Riboswitches. J Mol Biol 432(16):4523-4543 (2020)
Gong Z, Yang S, Dong X, Yang QF, Zhu YL, Xiao Y, Tang C
RgGuinier 1.6 nm
Dmax 6.7 nm
VolumePorod 12 nm3

SASDH88 – Wildtype preQ1 riboswitch in Thermoanaerobacter tengcongensis, holo-state - Tte-WT-holo

wildtype preQ1 riboswitch in Thermoanaerobacter tengcongensis experimental SAS data
wildtype preQ1 riboswitch in Thermoanaerobacter tengcongensis Kratky plot
Sample: Wildtype preQ1 riboswitch in Thermoanaerobacter tengcongensis monomer, 11 kDa Caldanaerobacter subterraneus subsp. … RNA
Buffer: 50 mM potassium phosphate, 2 mM MgCl2, 50 mM KCl, pH: 6.5
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2018 Mar 23
Hierarchical Conformational Dynamics Confers Thermal Adaptability to preQ1 RNA Riboswitches. J Mol Biol 432(16):4523-4543 (2020)
Gong Z, Yang S, Dong X, Yang QF, Zhu YL, Xiao Y, Tang C
RgGuinier 1.6 nm
Dmax 7.2 nm
VolumePorod 13 nm3

SASDH98 – Wildtype preQ1 riboswitch in Thermoanaerobacter tengcongensis, apo-state high-temperature sampling - Tte-WT-apo-HT

wildtype preQ1 riboswitch in Thermoanaerobacter tengcongensis experimental SAS data
wildtype preQ1 riboswitch in Thermoanaerobacter tengcongensis Kratky plot
Sample: Wildtype preQ1 riboswitch in Thermoanaerobacter tengcongensis monomer, 11 kDa Caldanaerobacter subterraneus subsp. … RNA
Buffer: 50 mM potassium phosphate, 2 mM MgCl2, 50 mM KCl, pH: 6.5
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2018 Mar 23
Hierarchical Conformational Dynamics Confers Thermal Adaptability to preQ1 RNA Riboswitches. J Mol Biol 432(16):4523-4543 (2020)
Gong Z, Yang S, Dong X, Yang QF, Zhu YL, Xiao Y, Tang C
RgGuinier 1.7 nm
Dmax 7.0 nm
VolumePorod 11 nm3