Small Angle Scattering Biological Data Bank SASBDB

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SASDKG4 – Malus domestica double bond reductase (MdDBR) apoform

Malus domestica double bond reductase experimental SAS data

Sample:	Malus domestica double bond reductase dimer, 77 kDa Malus domestica protein
Buffer:	50 mM Tris-HCl, 100 mM NaCl., pH: 7.5
Experiment:	SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2020 Oct 23

The structural and functional characterization of Malus domestica double bond reductase MdDBR provides insights towards the identification of its substrates International Journal of Biological Macromolecules 171:89-99 (2021)
Caliandro R, Polsinelli I, Demitri N, Musiani F, Martens S, Benini S

R_g^Guinier	3.0	nm
D_max	9.9	nm
Volume^Porod	110	nm³

SASDKH4 – Malus domestica double bond reductase in the presence of NADPH 5mM

Sample:	Malus domestica double bond reductase dimer, 77 kDa Malus domestica protein
Buffer:	50 mM Tris-HCl, 100 mM NaCl, 5 mM NADPH, pH: 7.5
Experiment:	SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2020 Oct 23

R_g^Guinier	3.1	nm
D_max	10.7	nm
Volume^Porod	99	nm³

SASDDH6 – Di-domain acyl carrier protein of the seryltransferase from prodigiosin biosynthesis

Di-domain acyl carrier protein of PigH from prodigiosin biosynthesis experimental SAS data

Sample:	Di-domain acyl carrier protein of PigH from prodigiosin biosynthesis monomer, 22 kDa Serratia sp. ATCC … protein
Buffer:	20 mM Tris supplemented with 5 mM DTT, pH: 7
Experiment:	SAXS data collected at BL1.3W, Synchrotron Light Research Institute (SLRI) on 2016 Jan 13

Solution Structure and Conformational Dynamics of a Doublet Acyl Carrier Protein from Prodigiosin Biosynthesis. Biochemistry (2021)
Thongkawphueak T, Winter AJ, Williams C, Maple HJ, Soontaranon S, Kaewhan C, Campopiano DJ, Crump MP, Wattana-Amorn P

R_g^Guinier	2.4	nm
D_max	8.4	nm
Volume^Porod	39	nm³

SASDJ49 – Apo PigH ACP1-ACP2

4-hydroxy-2,2'-bipyrrole-5-methanol synthase PigH experimental SAS data

Sample:	4-hydroxy-2,2'-bipyrrole-5-methanol synthase PigH monomer, 22 kDa Serratia sp. protein
Buffer:	25 mM Tris, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2020 Mar 3

R_g^Guinier	2.9	nm
D_max	9.9	nm
Volume^Porod	83	nm³

SASDJ59 – Holo PigH ACP1-ACP2

Sample:	4-hydroxy-2,2'-bipyrrole-5-methanol synthase PigH monomer, 22 kDa Serratia sp. protein
Buffer:	25 mM Tris, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2020 Mar 3

R_g^Guinier	2.8	nm
D_max	9.7	nm
Volume^Porod	72	nm³

SASDJ46 – Fep1-rec-GATA

GATA-type iron responsive transcription factor Fep1 reconstituted24-mer double strand DNA from the GATA promoter experimental SAS data

GATA-type iron responsive transcription factor Fep1 reconstituted 24-mer double strand DNA from the GATA promoter Kratky plot

Sample:	GATA-type iron responsive transcription factor Fep1 reconstituted monomer, 22 kDa Komagataella pastoris protein 24-mer double strand DNA from the GATA promoter dimer, 14 kDa synthetic oligonucleotide DNA
Buffer:	50 mM MOPS, 50 mM NaCl, pH: 7
Experiment:	SAXS data collected at BM29, ESRF on 2018 Dec 5

Biophysical characterization of the complex between the iron-responsive transcription factor Fep1 and DNA. Eur Biophys J (2021)
Miele AE, Cervoni L, Le Roy A, Cutone A, Musci G, Ebel C, Bonaccorsi di Patti MC

R_g^Guinier	3.5	nm
D_max	13.1	nm
Volume^Porod	73	nm³

SASDJ66 – GATA double strand DNA

24-mer double strand DNA from the GATA promoter experimental SAS data

Sample:	24-mer double strand DNA from the GATA promoter dimer, 14 kDa synthetic oligonucleotide DNA
Buffer:	50 mM MOPS, 50 mM NaCl, pH: 7
Experiment:	SAXS data collected at BM29, ESRF on 2018 Dec 5

R_g^Guinier	2.1	nm
D_max	7.4	nm
Volume^Porod	10	nm³

SASDJ86 – Fep1 wild type

GATA-type iron responsive transcription factor Fep1 experimental SAS data

Sample:	GATA-type iron responsive transcription factor Fep1 monomer, 22 kDa Komagataella pastoris protein
Buffer:	50 mM MOPS, 50 mM NaCl, pH: 7
Experiment:	SAXS data collected at BM29, ESRF on 2018 Dec 5

R_g^Guinier	3.5	nm
D_max	11.8	nm
Volume^Porod	41	nm³

SASDJ56 – Fep1-GATA

GATA-type iron responsive transcription factor Fep124-mer double strand DNA from the GATA promoter experimental SAS data

GATA-type iron responsive transcription factor Fep1 24-mer double strand DNA from the GATA promoter Kratky plot

Sample:	GATA-type iron responsive transcription factor Fep1 monomer, 22 kDa Komagataella pastoris protein 24-mer double strand DNA from the GATA promoter dimer, 14 kDa synthetic oligonucleotide DNA
Buffer:	50 mM MOPS, 50 mM NaCl, pH: 7
Experiment:	SAXS data collected at BM29, ESRF on 2018 Dec 5

R_g^Guinier	3.2	nm
D_max	12.0	nm
Volume^Porod	85	nm³

SASDJ76 – Fep1 wild type with [2Fe2S] cluster reconstituted

GATA-type iron responsive transcription factor Fep1 reconstituted experimental SAS data

Fep1 wild type with [2Fe2S] cluster reconstituted Rg histogram

Sample:	GATA-type iron responsive transcription factor Fep1 reconstituted monomer, 22 kDa Komagataella pastoris protein
Buffer:	50 mM MOPS, 50 mM NaCl, pH: 7
Experiment:	SAXS data collected at BM29, ESRF on 2018 Dec 5

R_g^Guinier	3.8	nm
D_max	16.5	nm
Volume^Porod	53	nm³

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