Browse by ORGANISM: other species

SASDJK2 – Artificial fusion protein of the beta-ketoacyl synthase Bamb_5920 C-terminal docking domain and the beta-ketoacyl synthase Bamb_5919 N-terminal docking domain

Beta-ketoacyl synthase Bamb_5920 CDD/Bamb_5919 NDD artificial protein fusion experimental SAS data
DAMFILT model
Sample: Beta-ketoacyl synthase Bamb_5920 CDD/Bamb_5919 NDD artificial protein fusion dimer, 13 kDa Burkholderia ambifaria protein
Buffer: 20 mM Tris-HCl, 200 mM NaCl, 5% glycerol, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Jan 23
Towards improved understanding of intersubunit interactions in modular polyketide biosynthesis: docking in the enacyloxin IIa polyketide synthase. J Struct Biol :107581 (2020)
Risser F, Collin S, Dos Santos-Morais R, Gruez A, Chagot B, Weissman KJ
RgGuinier 1.8 nm
Dmax 6.0 nm
VolumePorod 25 nm3

SASDJL2 – Artificial fusion protein of the beta-ketoacyl synthase Bamb_5925 C-terminal docking domain and the beta-ketoacyl synthase Bamb_5924 N-terminal docking domain

Beta-ketoacyl synthase Bamb_5925 CDD/Bamb_5924 NDD artificial protein fusion experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Beta-ketoacyl synthase Bamb_5925 CDD/Bamb_5924 NDD artificial protein fusion dimer, 18 kDa Burkholderia ambifaria protein
Buffer: 20 mM Tris-HCl, 200 mM NaCl, 5% glycerol, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Jan 23
Towards improved understanding of intersubunit interactions in modular polyketide biosynthesis: docking in the enacyloxin IIa polyketide synthase. J Struct Biol :107581 (2020)
Risser F, Collin S, Dos Santos-Morais R, Gruez A, Chagot B, Weissman KJ
RgGuinier 2.1 nm
Dmax 9.6 nm
VolumePorod 47 nm3

SASDHG9 – Trehalose transferase (TreT) from Thermoproteus uzoniensis - apo form

Trehalose transferase (Trehalose phosphorylase/synthase) experimental SAS data
DAMMIN model
Sample: Trehalose transferase (Trehalose phosphorylase/synthase) monomer, 47 kDa Thermoproteus uzoniensis protein
Buffer: 50 mM HEPES, 100 NaCl, 4 mM DTT,, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Sep 27
Anomeric Selectivity of Trehalose Transferase with Rare L-Sugars ACS Catalysis (2020)
Mestrom L, Marsden S, van der Eijk H, Laustsen J, Jeffries C, Svergun D, Hagedoorn P, Bento I, Hanefeld U
RgGuinier 2.5 nm
Dmax 8.8 nm
VolumePorod 77 nm3

SASDHH9 – Trehalose transferase (TreT) from Thermoproteus uzoniensis - with magnesium

Trehalose transferase (Trehalose phosphorylase/synthase) experimental SAS data
DAMMIN model
Sample: Trehalose transferase (Trehalose phosphorylase/synthase) monomer, 47 kDa Thermoproteus uzoniensis protein
Buffer: 50 mM HEPES, 100 NaCl, 4 mM DTT, 20 mM MgCl2, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Sep 27
Anomeric Selectivity of Trehalose Transferase with Rare L-Sugars ACS Catalysis (2020)
Mestrom L, Marsden S, van der Eijk H, Laustsen J, Jeffries C, Svergun D, Hagedoorn P, Bento I, Hanefeld U
RgGuinier 2.4 nm
Dmax 8.5 nm
VolumePorod 76 nm3

SASDHJ9 – Trehalose transferase (TreT) from Thermoproteus uzoniensis - with magnesium and trehalose

Trehalose transferase (Trehalose phosphorylase/synthase) experimental SAS data
DAMMIN model
Sample: Trehalose transferase (Trehalose phosphorylase/synthase) monomer, 47 kDa Thermoproteus uzoniensis protein
Buffer: 50 mM HEPES, 100 NaCl, 4 mM DTT, 20 mM MgCl2, 1 mM trehalose, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Sep 27
Anomeric Selectivity of Trehalose Transferase with Rare L-Sugars ACS Catalysis (2020)
Mestrom L, Marsden S, van der Eijk H, Laustsen J, Jeffries C, Svergun D, Hagedoorn P, Bento I, Hanefeld U
RgGuinier 2.4 nm
Dmax 8.3 nm
VolumePorod 76 nm3

SASDHK9 – Trehalose transferase (TreT) from Thermoproteus uzoniensis - with magnesium and UDP-glucose

Trehalose transferase (Trehalose phosphorylase/synthase) experimental SAS data
DAMMIN model
Sample: Trehalose transferase (Trehalose phosphorylase/synthase) monomer, 47 kDa Thermoproteus uzoniensis protein
Buffer: 50 mM HEPES, 100 NaCl, 4 mM DTT, 20 mM MgCl2, 1 mM UDP-glucose, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Sep 27
Anomeric Selectivity of Trehalose Transferase with Rare L-Sugars ACS Catalysis (2020)
Mestrom L, Marsden S, van der Eijk H, Laustsen J, Jeffries C, Svergun D, Hagedoorn P, Bento I, Hanefeld U
RgGuinier 2.4 nm
Dmax 8.5 nm
VolumePorod 73 nm3

SASDHJ7 – Upstream of N-ras, isoform A, CSD 4, 5 and 6 from Drosophila melanogaster

Upstream of N-ras, isoform A experimental SAS data
Upstream of N-ras, isoform A Kratky plot
Sample: Upstream of N-ras, isoform A monomer, 29 kDa Drosophila melanogaster protein
Buffer: 20 mM HEPES, 150 mM NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Sep 15
Pseudo-RNA-Binding Domains Mediate RNA Structure Specificity in Upstream of N-Ras. Cell Rep 32(3):107930 (2020)
Hollmann NM, Jagtap PKA, Masiewicz P, Guitart T, Simon B, Provaznik J, Stein F, Haberkant P, Sweetapple LJ, Villacorta L, Mooijman D, Benes V, Savitski MM, Gebauer F, Hennig J
RgGuinier 2.7 nm
Dmax 10.0 nm
VolumePorod 38 nm3

SASDHK7 – Upstream of N-ras, isoform A, CSD 7 and 8 from Drosophila melanogaster

Upstream of N-ras, isoform A experimental SAS data
Upstream of N-ras, isoform A Kratky plot
Sample: Upstream of N-ras, isoform A monomer, 19 kDa Drosophila melanogaster protein
Buffer: 20 mM HEPES, 150 mM NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 May 14
Pseudo-RNA-Binding Domains Mediate RNA Structure Specificity in Upstream of N-Ras. Cell Rep 32(3):107930 (2020)
Hollmann NM, Jagtap PKA, Masiewicz P, Guitart T, Simon B, Provaznik J, Stein F, Haberkant P, Sweetapple LJ, Villacorta L, Mooijman D, Benes V, Savitski MM, Gebauer F, Hennig J
RgGuinier 1.8 nm
Dmax 6.0 nm
VolumePorod 28 nm3

SASDHR4 – A structural characterization of poly(aspartic acid) hydrolase-1 from Sphingomonas sp. KT-1

Poly(Aspartic acid) hydrolase-1 experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Poly(Aspartic acid) hydrolase-1 dimer, 65 kDa Sphingomonas sp. KT-1 protein
Buffer: 20 mM Tris pH 7.0, 100 mM NaCl, 1 mM DTT, pH: 7
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Nov 27
Structural Characterization of Sphingomonas sp. KT-1 PahZ1-Catalyzed Biodegradation of Thermally Synthesized Poly(aspartic acid) ACS Sustainable Chemistry & Engineering (2020)
Brambley C, Bolay A, Salvo H, Jansch A, Yared T, Miller J, Wallen J, Weiland M
RgGuinier 2.7 nm
Dmax 8.9 nm
VolumePorod 66 nm3

SASDHP8 – Pentafunctional AROM polypeptide from Chaetomium thermophilum

Pentafunctional AROM polypeptide experimental SAS data
Pentafunctional AROM polypeptide Kratky plot
Sample: Pentafunctional AROM polypeptide dimer, 345 kDa Chaetomium thermophilum protein
Buffer: 20 mM HEPES, 150 mM NaCl. 2 mM TCEP, 1% sucrose, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2015 Nov 22
Architecture and functional dynamics of the pentafunctional AROM complex Nature Chemical Biology (2020)
Arora Verasztó H, Logotheti M, Albrecht R, Leitner A, Zhu H, Hartmann M
RgGuinier 5.4 nm
Dmax 17.5 nm
VolumePorod 623 nm3