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SASDWY6 – SEC-SAXS data for presumably disulfide-bounded dimers (2 x 24mer) of horse spleen apoferritin

Ferritin light chain experimental SAS data
CHIMERA model
Sample: Ferritin light chain, 953 kDa Equus caballus protein
Buffer: 137 mM NaCl, 2.7 mM KCl, 10 mM phosphate buffer, 0.9 mM CaCl2, 0.5 mM MgCl2, pH: 7.4
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2024 May 24
Structural insights into the nature of static and dynamic apoferritin dimers
Aleksei Tsarenko
RgGuinier 8.1 nm
Dmax 25.0 nm
VolumePorod 1677 nm3

SASDX83 – T. brucei Canonical Nucleosome Core Particle

Histone H2AHistone H2BHistone H3, putativeHistone H4Widom 601 145 bp DNA - strand 1Widom 601 145 bp DNA - strand 2 experimental SAS data
Histone H2A Histone H2B Histone H3, putative Histone H4 Widom 601 145 bp DNA - strand 1 Widom 601 145 bp DNA - strand 2 Kratky plot
Sample: Histone H2A, 28 kDa Trypanosoma brucei brucei … protein
Histone H2B, 25 kDa Trypanosoma brucei brucei … protein
Histone H3, putative, 29 kDa Trypanosoma brucei brucei … protein
Histone H4, 22 kDa Trypanosoma brucei brucei … protein
Widom 601 145 bp DNA - strand 1, 45 kDa DNA
Widom 601 145 bp DNA - strand 2, 45 kDa DNA
Buffer: 20 mM HEPES, 150 mM NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Oct 10
Trypanosome histone variants H3.V and H4.V promote nucleosome plasticity in repressed chromatin
RgGuinier 4.3 nm
Dmax 11.8 nm
VolumePorod 435 nm3

SASDX93 – T. brucei H3.V+H4.V Nucleosome Core Particle

Histone H2AHistone H2BWidom 601 145 bp DNA - strand 1Widom 601 145 bp DNA - strand 2Histone H3 variantHistone H4 experimental SAS data
Histone H2A Histone H2B Widom 601 145 bp DNA - strand 1 Widom 601 145 bp DNA - strand 2 Histone H3 variant Histone H4 Kratky plot
Sample: Histone H2A, 28 kDa Trypanosoma brucei brucei … protein
Histone H2B, 25 kDa Trypanosoma brucei brucei … protein
Widom 601 145 bp DNA - strand 1, 45 kDa DNA
Widom 601 145 bp DNA - strand 2, 45 kDa DNA
Histone H3 variant, 32 kDa Trypanosoma brucei brucei … protein
Histone H4, 22 kDa Trypanosoma brucei brucei … protein
Buffer: 20 mM HEPES, 150 mM NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Oct 10
Trypanosome histone variants H3.V and H4.V promote nucleosome plasticity in repressed chromatin
RgGuinier 4.5 nm
Dmax 13.1 nm
VolumePorod 483 nm3

SASDXA3 – T. brucei H3.V-only Nucleosome Core Particle

Histone H2AHistone H2BHistone H4Widom 601 145 bp DNA - strand 1Widom 601 145 bp DNA - strand 2Histone H3 variant experimental SAS data
Histone H2A Histone H2B Histone H4 Widom 601 145 bp DNA - strand 1 Widom 601 145 bp DNA - strand 2 Histone H3 variant Kratky plot
Sample: Histone H2A, 28 kDa Trypanosoma brucei brucei … protein
Histone H2B, 25 kDa Trypanosoma brucei brucei … protein
Histone H4, 22 kDa Trypanosoma brucei brucei … protein
Widom 601 145 bp DNA - strand 1, 45 kDa DNA
Widom 601 145 bp DNA - strand 2, 45 kDa DNA
Histone H3 variant, 32 kDa Trypanosoma brucei brucei … protein
Buffer: 20 mM HEPES, 150 mM NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Oct 10
Trypanosome histone variants H3.V and H4.V promote nucleosome plasticity in repressed chromatin
Gauri Deak
RgGuinier 4.4 nm
Dmax 13.2 nm
VolumePorod 481 nm3

SASDXB2 – Phosphoglycerate kinase

Phosphoglycerate kinase experimental SAS data
ALPHAFOLD model
Sample: Phosphoglycerate kinase monomer, 45 kDa Nakaseomyces glabratus protein
Buffer: 50 mM sodium phosphate, 500 mM NaCl, 500 mM imidazole, 1 mM phenylmethylsulfonyl fluoride (PMSF), pH: 8.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2025 Feb 13
Integrative Structural Characterization of Candida glabrata Phosphoglycerate Kinase by Small-Angle X-ray Scattering and AlphaFold: Implications for Therapeutic Targeting in Candidiasis ACS Omega (2026)
Cuéllar-Cruz M, Maqueda Cabrera E, Siliqi D, Moreno A
RgGuinier 2.9 nm
Dmax 10.3 nm
VolumePorod 79 nm3

SASDXB9 – O’Nyong-Nyong virus (ONNV) nsP4 full length wild type

RNA-directed RNA polymerase nsP4 experimental SAS data
RNA-directed RNA polymerase nsP4 Kratky plot
Sample: RNA-directed RNA polymerase nsP4 monomer, 68 kDa O'nyong-nyong virus protein
Buffer: 25 mM HEPES, 1 mM TCEP, 5% glycerol and 500 mM NaCl., pH: 7.5
Experiment: SAXS data collected at Rigaku BioSAXS-2000, Pennsylvania State University on 2023 Jun 8
A fold switch regulates conformation of an alphavirus RNA-dependent RNA polymerase. Nucleic Acids Res 54(2) (2026)
Arnold JJ, Braet SM, Vieira LC, Moustafa IM, Gohara DW, Fecko JA, Su YN, Jain A, Aponte-Diaz D, Wilke CO, Anand GS, Yennawar NH, Cameron CE
RgGuinier 3.6 nm

SASDXC9 – O'Nyong Nyong virus C-terminus-50residues-nsP3-nsP4 fusion protein

50-residue-C-terminus-nsP3 fused with RNA-directed RNA polymerase nsP4 experimental SAS data
50-residue-C-terminus-nsP3 fused with RNA-directed RNA polymerase nsP4 Kratky plot
Sample: 50-residue-C-terminus-nsP3 fused with RNA-directed RNA polymerase nsP4 tetramer, 318 kDa O'nyong nyong virus protein
Buffer: 25 mM HEPES 5% glycerol, 1 mM TCEP, 500 mM NaCl., pH: 7.5
Experiment: SAXS data collected at Rigaku BioSAXS-2000, Pennsylvania State University on 2025 Aug 14
A fold switch regulates conformation of an alphavirus RNA-dependent RNA polymerase. Nucleic Acids Res 54(2) (2026)
Arnold JJ, Braet SM, Vieira LC, Moustafa IM, Gohara DW, Fecko JA, Su YN, Jain A, Aponte-Diaz D, Wilke CO, Anand GS, Yennawar NH, Cameron CE
RgGuinier 5.2 nm

SASDN24 – Heparin oligosaccharide DP10

Heparine oligosaccharide of 10 sugar units (5 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan experimental SAS data
Heparine oligosaccharide of 10 sugar units (5 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan Kratky plot
Sample: Heparine oligosaccharide of 10 sugar units (5 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan monomer, 3 kDa Sus scrofa domesticus
Buffer: 10 mM HEPES, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2018 May 6
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 1.3 nm
Dmax 5.7 nm

SASDN34 – Heparin oligosaccharide DP12 in 50 mM Tris/HCl, pH 7.4

Heparine oligosaccharide of 12 sugar units (6 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan experimental SAS data
Heparine oligosaccharide of 12 sugar units (6 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan Kratky plot
Sample: Heparine oligosaccharide of 12 sugar units (6 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan monomer, 4 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2020 Nov 26
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 1.5 nm
Dmax 8.5 nm

SASDN44 – Heparin oligosaccharide DP12 in 50 mM Tris/HCl, 30 mM CaCl2, pH 7.4

Heparine oligosaccharide of 12 sugar units (6 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan experimental SAS data
Heparine oligosaccharide of 12 sugar units (6 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan Kratky plot
Sample: Heparine oligosaccharide of 12 sugar units (6 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan monomer, 4 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, 30 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2020 Nov 26
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 1.5 nm
Dmax 6.8 nm