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SASDUM2 – mRNA capping enzyme MTase

Virus termination factor small subunitmRNA-capping enzyme catalytic subunit experimental SAS data
DAMFILT model
Sample: Virus termination factor small subunit monomer, 33 kDa Monkeypox virus (strain … protein
MRNA-capping enzyme catalytic subunit monomer, 35 kDa Monkeypox virus (strain … protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2024 Jan 4
Structural basis of the monkeypox virus mRNA cap N7 methyltransferase complex. Emerg Microbes Infect 13(1):2369193 (2024)
Chen A, Fang N, Zhang Z, Wen Y, Shen Y, Zhang Y, Zhang L, Zhao G, Ding J, Li J
RgGuinier 2.9 nm
Dmax 10.1 nm
VolumePorod 106 nm3

SASDU78 – Trypanosoma brucei ESAG4 membrane-proximal Venus Fly Trap domain 2 (VFT2)

adenylate cyclase experimental SAS data
ALPHAFOLD model
Sample: Adenylate cyclase monomer, 43 kDa Trypanosoma brucei protein
Buffer: 50 mM Tris-HCl, 500 mM NaCl, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2023 Sep 23
Biophysical analysis of the membrane-proximal Venus Flytrap domain of ESAG4 receptor-like adenylate cyclase from Trypanosoma brucei. Mol Biochem Parasitol 260:111653 (2024)
Alves DO, Geens R, da Silva Arruda HR, Jennen L, Corthaut S, Wuyts E, de Andrade GC, Prosdocimi F, Cordeiro Y, Pires JR, Vieira LR, de Oliveira GAP, Sterckx YG, Salmon D
RgGuinier 2.3 nm
Dmax 7.6 nm
VolumePorod 76 nm3

SASDVT9 – BioSAXS Analysis of Xylose Isomerase from Bacteroides thetaiotaomicron in PBS

Xylose isomerase experimental SAS data
DAMMIN model
Sample: Xylose isomerase tetramer, 196 kDa Bacteroides thetaiotaomicron (strain … protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at BioSAXS, Australian Synchrotron on 2024 Oct 22
BioSAXS Australian Synchrotron Standard Protein
Annmaree Warrender
RgGuinier 3.5 nm
Dmax 9.9 nm
VolumePorod 222 nm3

SASDVX2 – Full-length SARS-CoV-2 5'ge element stem-loop 5 (5_SL5)

full stem-loop 5 of SARS-CoV-2 5'genomic end experimental SAS data
PYMOL model
Sample: Full stem-loop 5 of SARS-CoV-2 5'genomic end monomer, 48 kDa Severe acute respiratory … RNA
Buffer: 25 mM potassium phosphate, 50 mM KCl, pH: 6.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Aug 7
Dissecting the Conformational Heterogeneity of Stem-Loop Substructures of the Fifth Element in the 5'-Untranslated Region of SARS-CoV-2. J Am Chem Soc 146(44):30139-30154 (2024)
Mertinkus KR, Oxenfarth A, Richter C, Wacker A, Mata CP, Carazo JM, Schlundt A, Schwalbe H
RgGuinier 4.3 nm
Dmax 13.8 nm

SASDVY2 – Sub-element stem-loop 5a within the SARS-CoV-2 5'ge element stem-loop 5 (5_SL5)

sub-element stem-loop 5a from SARS-CoV-2 5'genomic end experimental SAS data
PYMOL model
Sample: Sub-element stem-loop 5a from SARS-CoV-2 5'genomic end monomer, 11 kDa Severe acute respiratory … RNA
Buffer: 25 mM potassium phosphate, 50 mM KCl, pH: 6.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Aug 7
Dissecting the Conformational Heterogeneity of Stem-Loop Substructures of the Fifth Element in the 5'-Untranslated Region of SARS-CoV-2. J Am Chem Soc 146(44):30139-30154 (2024)
Mertinkus KR, Oxenfarth A, Richter C, Wacker A, Mata CP, Carazo JM, Schlundt A, Schwalbe H
RgGuinier 1.9 nm
Dmax 6.0 nm

SASDVC3 – Sub-element stem-loop 5b within the SARS-CoV-2 5'ge element stem-loop 5 (5_SL5)

sub-element stem-loop 5b from SARS-CoV-2 5'genomic end experimental SAS data
PYMOL model
Sample: Sub-element stem-loop 5b from SARS-CoV-2 5'genomic end monomer, 8 kDa Severe acute respiratory … RNA
Buffer: 25 mM potassium phosphate, 50 mM KCl, pH: 6.2
Experiment: SAXS data collected at BM29, ESRF on 2021 Feb 3
Dissecting the Conformational Heterogeneity of Stem-Loop Substructures of the Fifth Element in the 5'-Untranslated Region of SARS-CoV-2. J Am Chem Soc 146(44):30139-30154 (2024)
Mertinkus KR, Oxenfarth A, Richter C, Wacker A, Mata CP, Carazo JM, Schlundt A, Schwalbe H
RgGuinier 1.4 nm
Dmax 4.3 nm

SASDS66 – Solution conformation of Harpin HrpZ2 protein from Pseudomonas syringae, a plant pathogen

Harpin Z2 experimental SAS data
CORAL model
Sample: Harpin Z2 monomer, 37 kDa Pseudomonas syringae strain … protein
Buffer: 10 mM MES, 100 mM NaF, pH: 6.2
Experiment: SAXS data collected at Rigaku BioSAXS-2000, Centre for Cellular and Molecular Biology on 2023 May 1
Solution conformation of HrpZ2 protein from Pseudomonas syringae
Arpita Goswami
RgGuinier 3.1 nm
Dmax 8.8 nm
VolumePorod 64 nm3

SASDS37 – Oligomerization initiating solution conformation of Harpin HrpZ2 protein from Pseudomonas syringae, a plant pathogen

Harpin Z2 experimental SAS data
CORAL model
Sample: Harpin Z2 monomer, 37 kDa Pseudomonas syringae strain … protein
Buffer: 10 mM MES, 100 mM NaF, pH: 6.2
Experiment: SAXS data collected at Rigaku BioSAXS-2000, Centre for Cellular and Molecular Biology on 2023 May 1
Solution conformation of HrpZ2 protein from Pseudomonas syringae
Arpita Goswami
RgGuinier 3.2 nm
Dmax 11.4 nm
VolumePorod 70 nm3

SASDCN8 – SAXS data of Legionella pneumophila phosphocholine hydrolase Lem3(19-570)

Phosphocholine hydrolase Lem3 experimental SAS data
Phosphocholine hydrolase Lem3 Kratky plot
Sample: Phosphocholine hydrolase Lem3 monomer, 63 kDa Legionella pneumophila subsp. … protein
Buffer: 300 mM NaCl, 2 mM 2-mercaptoethanol and 30 mM Tris-HCl, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2015 Sep 20
The structural analysis of dephosphocholinase Legionella pneumophila Lem3
Wenhua Zhang
RgGuinier 3.5 nm
Dmax 12.2 nm
VolumePorod 95 nm3

SASDTW8 – Staphylococcus aureus Fatty Acid Kinase A

Uncharacterized protein SAUSA300_1119 experimental SAS data
DAMMIF model
Sample: Uncharacterized protein SAUSA300_1119 dimer, 125 kDa Staphylococcus aureus (strain … protein
Buffer: 50 mM Tris, 150 mM KCl, 1 mM TCEP, 5% glycerol, pH: 7.4
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Oct 5
Molecular insights into the structure and function of the Staphylococcus aureus fatty acid kinase. J Biol Chem 300(12):107920 (2024)
Myers MJ, Xu Z, Ryan BJ, DeMars ZR, Ridder MJ, Johnson DK, Krute CN, Flynn TS, Kashipathy MM, Battaile KP, Schnicker N, Lovell S, Freudenthal BD, Bose JL
RgGuinier 4.3 nm
Dmax 16.0 nm
VolumePorod 219 nm3