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SASDN54 – Heparin oligosaccharide DP12 in 50 mM Tris/HCl, 60 mM CaCl2, pH 7.4

Heparine oligosaccharide of 12 sugar units (6 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan experimental SAS data
Heparine oligosaccharide of 12 sugar units (6 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan Kratky plot
Sample: Heparine oligosaccharide of 12 sugar units (6 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan monomer, 4 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, 60 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2020 Nov 26
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 1.4 nm
Dmax 8.6 nm

SASDN64 – Heparin oligosaccharide DP12 in 50 mM Tris/HCl, 120 mM CaCl2, pH 7.4

Heparine oligosaccharide of 12 sugar units (6 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan experimental SAS data
Heparine oligosaccharide of 12 sugar units (6 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan Kratky plot
Sample: Heparine oligosaccharide of 12 sugar units (6 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan monomer, 4 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, 120 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2020 Nov 26
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 1.5 nm
Dmax 6.8 nm

SASDN74 – Heparin oligosaccharide DP18 in 50 mM Tris/HCl, pH 7.4

Heparine oligosaccharide of 18 sugar units (9 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan experimental SAS data
Heparine oligosaccharide of 18 sugar units (9 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan Kratky plot
Sample: Heparine oligosaccharide of 18 sugar units (9 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan monomer, 5 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 1.8 nm
Dmax 6.3 nm

SASDN84 – Heparin oligosaccharide DP18 in 50 mM Tris/HCl, 30 mM CaCl2, pH 7.4

Heparine oligosaccharide of 18 sugar units (9 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan experimental SAS data
Heparine oligosaccharide of 18 sugar units (9 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan Kratky plot
Sample: Heparine oligosaccharide of 18 sugar units (9 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan monomer, 5 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, 30 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 1.8 nm
Dmax 7.7 nm

SASDN94 – Heparin oligosaccharide DP18 in 50 mM Tris/HCl, 60 mM CaCl2, pH 7.4

Heparine oligosaccharide of 18 sugar units (9 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan experimental SAS data
Heparine oligosaccharide of 18 sugar units (9 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan Kratky plot
Sample: Heparine oligosaccharide of 18 sugar units (9 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan monomer, 5 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, 60 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 1.8 nm
Dmax 7.9 nm

SASDNA4 – Heparin oligosaccharide DP18 in 50 mM Tris/HCl, 120 mM CaCl2, pH 7.4

Heparine oligosaccharide of 18 sugar units (9 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan experimental SAS data
Heparine oligosaccharide of 18 sugar units (9 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan Kratky plot
Sample: Heparine oligosaccharide of 18 sugar units (9 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan monomer, 5 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, 120 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 1.8 nm
Dmax 7.5 nm

SASDNB4 – Heparin oligosaccharide in 50 mM Tris/HCl pH 7.4, 1.25 mg/mL

Heparin experimental SAS data
Heparin Kratky plot
Sample: Heparin monomer, 14 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 4.8 nm
Dmax 29.7 nm

SASDNC4 – Heparin oligosaccharide in 50 mM Tris/HCl pH 7.4, 2.5 mg/mL

Heparin experimental SAS data
Heparin Kratky plot
Sample: Heparin monomer, 14 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 4.8 nm
Dmax 23.9 nm

SASDND4 – Heparin oligosaccharide in 50 mM Tris/HCl pH 7.4, 5mg/mL

Heparin experimental SAS data
Heparin Kratky plot
Sample: Heparin monomer, 14 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 3.7 nm
Dmax 23.7 nm

SASDNE4 – Heparin oligosaccharide in 50 mM Tris/HCl pH 7.4, 10 mg/mL

Heparin experimental SAS data
Heparin Kratky plot
Sample: Heparin monomer, 14 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 2.4 nm
Dmax 20.8 nm