Browse by ORGANISM: other species

SASDA67 – Surface Protein G (SasG) EG5 repeat protein G51-G55

Surface protein G experimental SAS data
SASREF model
Sample: Surface protein G monomer, 67 kDa Staphylococcus aureus protein
Buffer: 20 mM Tris 200 mM NaCl 1 mM EDTA 20 mM Tris.Cl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 12
Cooperative folding of intrinsically disordered domains drives assembly of a strong elongated protein. Nat Commun 6:7271 (2015)
Gruszka DT, Whelan F, Farrance OE, Fung HK, Paci E, Jeffries CM, Svergun DI, Baldock C, Baumann CG, Brockwell DJ, Potts JR, Clarke J
RgGuinier 12.0 nm
Dmax 48.0 nm
VolumePorod 87 nm3

SASDA77 – Surface Protein G (SasG) EG5 repeat protein G51-G56

Surface protein G experimental SAS data
SASREF model
Sample: Surface protein G monomer, 81 kDa Staphylococcus aureus protein
Buffer: 20 mM Tris 200 mM NaCl 1 mM EDTA 20 mM Tris.Cl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 12
Cooperative folding of intrinsically disordered domains drives assembly of a strong elongated protein. Nat Commun 6:7271 (2015)
Gruszka DT, Whelan F, Farrance OE, Fung HK, Paci E, Jeffries CM, Svergun DI, Baldock C, Baumann CG, Brockwell DJ, Potts JR, Clarke J
RgGuinier 14.1 nm
Dmax 57.0 nm
VolumePorod 89 nm3

SASDA87 – Surface Protein G (SasG) EG5 repeat protein G51-G57

Surface protein G experimental SAS data
SASREF model
Sample: Surface protein G monomer, 95 kDa Staphylococcus aureus protein
Buffer: 20 mM Tris 200 mM NaCl 1 mM EDTA 20 mM Tris.Cl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 12
Cooperative folding of intrinsically disordered domains drives assembly of a strong elongated protein. Nat Commun 6:7271 (2015)
Gruszka DT, Whelan F, Farrance OE, Fung HK, Paci E, Jeffries CM, Svergun DI, Baldock C, Baumann CG, Brockwell DJ, Potts JR, Clarke J
RgGuinier 15.9 nm
Dmax 63.0 nm
VolumePorod 122 nm3

SASDA97 – PlaB

PlaB experimental SAS data
DAMMIN model
Sample: PlaB tetramer, 220 kDa Legionella pneumophila protein
Buffer: 100 mM Tris 100 mM Nacl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 15
Automated pipeline for purification, biophysical and x-ray analysis of biomacromolecular solutions. Sci Rep 5:10734 (2015)
Graewert MA, Franke D, Jeffries CM, Blanchet CE, Ruskule D, Kuhle K, Flieger A, Schäfer B, Tartsch B, Meijers R, Svergun DI
RgGuinier 4.0 nm
Dmax 10.5 nm
VolumePorod 270 nm3

SASDC73 – Atg1-Atg13-Atg17-Atg31-Atg29 Minipentamer Complex

Serine/threonine-protein kinase ATG1Autophagy-related protein 13Autophagy-related protein 17Autophagy-related protein 29KLLA0A10637p experimental SAS data
Serine/threonine-protein kinase ATG1 Autophagy-related protein 13 Autophagy-related protein 17 Autophagy-related protein 29 KLLA0A10637p Kratky plot
Sample: Serine/threonine-protein kinase ATG1 dimer, 61 kDa Kluyveromyces lactis protein
Autophagy-related protein 13 dimer, 17 kDa Kluyveromyces lactis protein
Autophagy-related protein 17 dimer, 100 kDa Kluyveromyces lactis protein
Autophagy-related protein 29 dimer, 20 kDa Kluyveromyces lactis protein
KLLA0A10637p dimer, 32 kDa Kluyveromyces lactis protein
Buffer: 20 mM Tris, 200 mM NaCl, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2013 Dec 20
Solution structure of the Atg1 complex: implications for the architecture of the phagophore assembly site. Structure 23(5):809-818 (2015)
Köfinger J, Ragusa MJ, Lee IH, Hummer G, Hurley JH
RgGuinier 10.3 nm
Dmax 34.0 nm
VolumePorod 1000 nm3

SASDCL3 – Atg1-Atg13 Subcomplex

Serine/threonine-protein kinase ATG1Autophagy-related protein 13 experimental SAS data
Serine/threonine-protein kinase ATG1 Autophagy-related protein 13 Kratky plot
Sample: Serine/threonine-protein kinase ATG1 dimer, 61 kDa Kluyveromyces lactis protein
Autophagy-related protein 13 dimer, 17 kDa Kluyveromyces lactis protein
Buffer: 20 mM Tris, 200 mM NaCl, 2% glycerol, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2013 Dec 10
Solution structure of the Atg1 complex: implications for the architecture of the phagophore assembly site. Structure 23(5):809-818 (2015)
Köfinger J, Ragusa MJ, Lee IH, Hummer G, Hurley JH
RgGuinier 3.3 nm
Dmax 10.9 nm
VolumePorod 160 nm3

SASDCM3 – Atg17-Atg31-Atg29 Subcomplex

Autophagy-related protein 17Autophagy-related protein 29KLLA0A10637p experimental SAS data
Autophagy-related protein 17 Autophagy-related protein 29 KLLA0A10637p Kratky plot
Sample: Autophagy-related protein 17 dimer, 100 kDa Kluyveromyces lactis protein
Autophagy-related protein 29 dimer, 20 kDa Kluyveromyces lactis protein
KLLA0A10637p dimer, 32 kDa Kluyveromyces lactis protein
Buffer: 20 mM Tris, 200 mM NaCl, 2% glycerol, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2013 Dec 10
Solution structure of the Atg1 complex: implications for the architecture of the phagophore assembly site. Structure 23(5):809-818 (2015)
Köfinger J, Ragusa MJ, Lee IH, Hummer G, Hurley JH
RgGuinier 10.1 nm
Dmax 34.0 nm
VolumePorod 848 nm3

SASDA96 – Lysozyme

Lysozyme C experimental SAS data
DAMMIF model
Sample: Lysozyme C monomer, 14 kDa Gallus gallus protein
Buffer: 20 mM Sodium Acetate/HEPES, pH: 6.8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Feb 17
Correlation Map, a goodness-of-fit test for one-dimensional X-ray scattering spectra. Nat Methods 12(5):419-22 (2015)
Franke D, Jeffries CM, Svergun DI
RgGuinier 1.5 nm
Dmax 4.8 nm
VolumePorod 24 nm3

SASDAB6 – Xylose Isomerase

Xylose Isomerase experimental SAS data
DAMMIF model
Sample: Xylose Isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer: 20 mM HEPES 200 mM Na2SO4 50 mM K2SO4 500 % v/v D2O 1 mM MgCl2, pH: 6.6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Dec 10
Correlation Map, a goodness-of-fit test for one-dimensional X-ray scattering spectra. Nat Methods 12(5):419-22 (2015)
Franke D, Jeffries CM, Svergun DI
RgGuinier 3.2 nm
Dmax 9.1 nm
VolumePorod 234 nm3

SASDAL6 – Wild-type chalcone isomerase, ligand-free

Bacterial chalcone isomerase experimental SAS data
Bacterial chalcone isomerase Kratky plot
Sample: Bacterial chalcone isomerase hexamer, 194 kDa Eubacterium ramulus protein
Buffer: 50 mM sodium phosphate, pH: 6.8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Sep 23
Structure and catalytic mechanism of the evolutionarily unique bacterial chalcone isomerase. Acta Crystallogr D Biol Crystallogr 71(Pt 4):907-17 (2015)
Thomsen M, Tuukkanen A, Dickerhoff J, Palm GJ, Kratzat H, Svergun DI, Weisz K, Bornscheuer UT, Hinrichs W
RgGuinier 4.0 nm
Dmax 13.0 nm
VolumePorod 320 nm3