Small Angle Scattering Biological Data Bank SASBDB

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SASDRY8 – Single-chain Archaeoglobus fulgidus argonaute fAfAgo-fusion variant complexed with a 14 base pair DNA oligoduplex in low salt buffer

single-chain full Archaeoglobus fulgidus Argonaute5'-end phosphorylated DNA oligoduplex, 14 bp (MZ1288) experimental SAS data

Sample:	Single-chain full Archaeoglobus fulgidus Argonaute monomer, 78 kDa Archaeoglobus fulgidus protein 5'-end phosphorylated DNA oligoduplex, 14 bp (MZ1288) monomer, 9 kDa DNA
Buffer:	20 mM TrisHCl pH7.5, 200 mM NaCl, 5 mM MgCl2, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Jul 6

The missing part: the Archaeoglobus fulgidus Argonaute forms a functional heterodimer with an N-L1-L2 domain protein. Nucleic Acids Res (2024)
Manakova E, Golovinas E, Pocevičiūtė R, Sasnauskas G, Silanskas A, Rutkauskas D, Jankunec M, Zagorskaitė E, Jurgelaitis E, Grybauskas A, Venclovas Č, Zaremba M

R_g^Guinier	3.0	nm
D_max	10.3	nm
Volume^Porod	131	nm³

SASDRZ8 – Single-chain Archaeoglobus fulgidus argonaute fAfAgo-fusion variant complexed with an 11 base pair DNA oligoduplex in low salt buffer

single-chain full Archaeoglobus fulgidus Argonaute5'-end phosphorylated DNA guide strand, 11 nt (MZ864)DNA target strand, 11 nt (MZ865) experimental SAS data

Sample:	Single-chain full Archaeoglobus fulgidus Argonaute monomer, 78 kDa Archaeoglobus fulgidus protein 5'-end phosphorylated DNA guide strand, 11 nt (MZ864) monomer, 3 kDa DNA DNA target strand, 11 nt (MZ865) monomer, 3 kDa DNA
Buffer:	20 mM TrisHCl pH7.5, 200 mM NaCl, 5 mM MgCl2, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Aug 31

R_g^Guinier	2.9	nm
D_max	9.6	nm
Volume^Porod	125	nm³

SASDR29 – Single-chain Archaeoglobus fulgidus argonaute fAfAgo-fusion variant in high salt buffer

single-chain full Archaeoglobus fulgidus Argonaute variant experimental SAS data

Sample:	Single-chain full Archaeoglobus fulgidus Argonaute variant monomer, 78 kDa Archaeoglobus fulgidus protein
Buffer:	20 mM TrisHCl pH 7.5, 500 mM NaCl, 5 mM MgCl2, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Aug 31

R_g^Guinier	3.1	nm
D_max	9.7	nm
Volume^Porod	142	nm³

SASDPK3 – Vibrio cholerae ParD2:ParE2 complex bound to 21-bp DNA operator box

Antitoxin ParDToxin21-bp DNA operator fragment experimental SAS data

Sample:	Antitoxin ParD hexamer, 54 kDa Vibrio cholerae serotype … protein Toxin, 25 kDa Vibrio cholerae serotype … protein 21-bp DNA operator fragment monomer, 13 kDa Vibrio cholerae O1 DNA
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2020 Jul 18

Toxin:antitoxin ratio sensing autoregulation of the Vibrio cholerae parDE2 module. Sci Adv 10(1):eadj2403 (2024)
Garcia-Rodriguez G, Girardin Y, Kumar Singh R, Volkov AN, Van Dyck J, Muruganandam G, Sobott F, Charlier D, Loris R

R_g^Guinier	3.2	nm
D_max	10.0	nm
Volume^Porod	140	nm³

SASDPL3 – Vibrio cholerae ParD2:ParE2 complex bound to 31-bp DNA operator box

Antitoxin ParDToxin31-bp DNA operator box experimental SAS data

Sample:	Antitoxin ParD hexamer, 54 kDa Vibrio cholerae serotype … protein Toxin, 25 kDa Vibrio cholerae serotype … protein 31-bp DNA operator box monomer, 19 kDa Vibrio cholerae O1 DNA
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2019 Dec 4

R_g^Guinier	3.3	nm
D_max	10.5	nm
Volume^Porod	160	nm³

SASDPM3 – Vibrio cholerae ParD2:ParE2 complex bound to 33-bp DNA operator fragment

Antitoxin ParDToxin33-bp DNA operator fragment experimental SAS data

Sample:	Antitoxin ParD hexamer, 54 kDa Vibrio cholerae serotype … protein Toxin, 25 kDa Vibrio cholerae serotype … protein 33-bp DNA operator fragment monomer, 20 kDa Vibrio cholerae O1 DNA
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2020 Jul 18

R_g^Guinier	3.2	nm
D_max	10.0	nm
Volume^Porod	150	nm³

SASDPN3 – Vibrio cholerae ParD2:ParE2 antitoxin:toxin complex

Antitoxin ParDToxin experimental SAS data

Sample:	Antitoxin ParD hexamer, 54 kDa Vibrio cholerae serotype … protein Toxin, 25 kDa Vibrio cholerae serotype … protein
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2017 Mar 6

R_g^Guinier	3.0	nm
D_max	11.0	nm
Volume^Porod	140	nm³

SASDPE2 – Starch adherence system protein 6 (Sas6)

Dockerin type I repeat experimental SAS data

Sample:	Dockerin type I repeat monomer, 69 kDa Ruminococcus bromii protein
Buffer:	phosphate buffered saline, 1 mM TCEP, pH: 7
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 14

The Ruminococcus bromii amylosome protein Sas6 binds single and double helical α-glucan structures in starch. Nat Struct Mol Biol (2024)
Photenhauer AL, Villafuerte-Vega RC, Cerqueira FM, Armbruster KM, Mareček F, Chen T, Wawrzak Z, Hopkins JB, Vander Kooi CW, Janeček Š, Ruotolo BT, Koropatkin NM

R_g^Guinier	3.0	nm
D_max	9.0	nm
Volume^Porod	97	nm³

SASDTG2 – LCP domain of LytR from Streptococcus dysgalactiae in the presence of geranylgeranyl diphosphate (150 mM NaCl)

Biofilm regulatory protein experimental SAS data

Sample:	Biofilm regulatory protein monomer, 34 kDa Streptococcus dysgalactiae subsp. … protein
Buffer:	50 mM HEPES, 150 mM NaCl, 5 mM MgCl2, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2022 Jun 1

Structural insights of an LCP protein-LytR-from Streptococcus dysgalactiae subs. dysgalactiae through biophysical and in silico methods. Front Chem 12:1379914 (2024)
Paquete-Ferreira J, Freire F, Fernandes HS, Muthukumaran J, Ramos J, Bryton J, Panjkovich A, Svergun D, Santos MFA, Correia MAS, Fernandes AR, Romão MJ, Sousa SF, Santos-Silva T

R_g^Guinier	2.5	nm
D_max	8.0	nm
Volume^Porod	83	nm³

SASDTH2 – LCP domain of LytR from Streptococcus dysgalactiae in the absence of ligand (150 mM NaCl)

Sample:	Biofilm regulatory protein monomer, 34 kDa Streptococcus dysgalactiae subsp. … protein
Buffer:	50 mM HEPES, 150 mM NaCl, 5 mM MgCl2, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2022 Jun 1

R_g^Guinier	2.4	nm
D_max	9.0	nm
Volume^Porod	76	nm³

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