Browse by ORGANISM: other species

SASDRD8 – Drosophila melanogaster kinesin heavy chain

Kinesin heavy chain (A515T) experimental SAS data
Kinesin heavy chain (A515T) Kratky plot
Sample: Kinesin heavy chain (A515T) dimer, 221 kDa Drosophila melanogaster protein
Buffer: 25 mM HEPES/KOH, 150 mM NaCl, 1 mM MgCl2, 2 mM DTT, pH: 7.3
Experiment: SAXS data collected at BM29, ESRF on 2022 Feb 19
Tropomyosin 1-I/C coordinates kinesin-1 and dynein motors during oskar mRNA transport. Nat Struct Mol Biol (2024)
Heber S, McClintock MA, Simon B, Mehtab E, Lapouge K, Hennig J, Bullock SL, Ephrussi A
RgGuinier 10.0 nm
Dmax 45.0 nm
VolumePorod 920 nm3

SASDRE8 – Drosophila melanogaster kinesin heavy chain + Tropomyosin1-I/C

Kinesin heavy chain (A515T)GH09289p experimental SAS data
Kinesin heavy chain (A515T) GH09289p Kratky plot
Sample: Kinesin heavy chain (A515T) dimer, 221 kDa Drosophila melanogaster protein
GH09289p dimer, 96 kDa Drosophila melanogaster protein
Buffer: 25 mM HEPES/KOH, 150 mM NaCl, 1 mM MgCl2, 2 mM DTT, pH: 7.3
Experiment: SAXS data collected at BM29, ESRF on 2022 Feb 19
Tropomyosin 1-I/C coordinates kinesin-1 and dynein motors during oskar mRNA transport. Nat Struct Mol Biol (2024)
Heber S, McClintock MA, Simon B, Mehtab E, Lapouge K, Hennig J, Bullock SL, Ephrussi A
RgGuinier 8.7 nm
Dmax 45.0 nm
VolumePorod 840 nm3

SASDT55 – Non-structural protein 1 (NS1B, C-terminal) with 5' PPP hairpin RNA

Non-structural protein 15’ppp ds10 HP RNA experimental SAS data
HADDOCK model
Sample: Non-structural protein 1 monomer, 16 kDa Influenza B virus protein
5’ppp ds10 HP RNA monomer, 8 kDa RNA
Buffer: 40 mM ammonium acetate, pH 5.5, 225 mM NaCl, 5 mM CaCl2, 0.02% NaN3, 50 mM arginine, pH: 5.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2018 Aug 6
The NS1 protein of influenza B virus binds 5’-triphosphorylated dsRNA to suppress RIG-I activation and the host antiviral response (2024)
Woltz R, Schweibenz B, Tsutakawa S, Zhao C, Ma L, Shurina B, Hura G, John R, Vorobiev S, Swapna G, Solotchi M, Tainer J, Krug R, Patel S, Montelione G
RgGuinier 2.0 nm
Dmax 6.9 nm
VolumePorod 28 nm3

SASDT65 – Non-structural protein 1 (NS1B, C-terminal) plus double stranded RNA

Non-structural protein 1RNA top strandRNA bottom strand experimental SAS data
HADDOCK model
Sample: Non-structural protein 1 monomer, 16 kDa Influenza B virus protein
RNA top strand monomer, 5 kDa RNA
RNA bottom strand monomer, 5 kDa RNA
Buffer: 40 mM ammonium acetate, pH 5.5, 450 mM NaCl, 5 mM CaCl2, 0.02% NaN3, 50 mM arginine, pH: 5.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 May 18
The NS1 protein of influenza B virus binds 5’-triphosphorylated dsRNA to suppress RIG-I activation and the host antiviral response (2024)
Woltz R, Schweibenz B, Tsutakawa S, Zhao C, Ma L, Shurina B, Hura G, John R, Vorobiev S, Swapna G, Solotchi M, Tainer J, Krug R, Patel S, Montelione G
RgGuinier 2.8 nm
Dmax 10.3 nm
VolumePorod 23 nm3

SASDT82 – Halophilic Lrp transcription factor

AsnC family transcriptional regulator experimental SAS data
PHENIX model
Sample: AsnC family transcriptional regulator dimer, 36 kDa Haloferax mediterranei (strain … protein
Buffer: 50 mM Tris, 1.5 M NaCl, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2021 Nov 12
Global Lrp regulator protein from Haloferax mediterranei: Transcriptional analysis and structural characterization. Int J Biol Macromol :129541 (2024)
Matarredona L, García-Bonete MJ, Guío J, Camacho M, Fillat MF, Esclapez J, Bonete MJ
RgGuinier 2.4 nm
Dmax 7.9 nm
VolumePorod 70 nm3

SASDEU9 – Legionella pneumophila Phosphocholinase AnkX

Phosphocholinase AnkX experimental SAS data
GASBOR model
Sample: Phosphocholinase AnkX monomer, 107 kDa Legionella pneumophila protein
Buffer: 300 mM NaCl, 2 mM 2-mercaptoethanol and 30 mM Tris-HCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Oct 20
Legionella pneumophila effector AnkX
Wenhua Zhang
RgGuinier 4.3 nm
Dmax 14.7 nm
VolumePorod 145 nm3

SASDRX8 – Heterodimeric complex of AfAgo protein Af1318 and AfAgo-N protein (containing N-L1-L2 domains) with a 14 base pair DNA oligoduplex in low salt buffer

Piwi protein AF_1318 (Archaeoglobus fulgidus AfAgo protein)Uncharacterized protein (AfAgo-N protein containing N-L1-L2 domains)5'-end phosphorylated DNA oligoduplex, 14 bp (MZ1288) experimental SAS data
CUSTOM IN-HOUSE model
Sample: Piwi protein AF_1318 (Archaeoglobus fulgidus AfAgo protein) monomer, 51 kDa Archaeoglobus fulgidus (strain … protein
Uncharacterized protein (AfAgo-N protein containing N-L1-L2 domains) monomer, 31 kDa Archaeoglobus fulgidus DSM … protein
5'-end phosphorylated DNA oligoduplex, 14 bp (MZ1288) monomer, 9 kDa DNA
Buffer: 20 mM TrisHCl pH 7.5, 200 mM NaCl, 5 mM MgCl2, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 6
The missing part: the Archaeoglobus fulgidus Argonaute forms a functional heterodimer with an N-L1-L2 domain protein. Nucleic Acids Res (2024)
Manakova E, Golovinas E, Pocevičiūtė R, Sasnauskas G, Silanskas A, Rutkauskas D, Jankunec M, Zagorskaitė E, Jurgelaitis E, Grybauskas A, Venclovas Č, Zaremba M
RgGuinier 2.9 nm
Dmax 9.7 nm
VolumePorod 145 nm3

SASDRY8 – Single-chain Archaeoglobus fulgidus argonaute fAfAgo-fusion variant complexed with a 14 base pair DNA oligoduplex in low salt buffer

single-chain full Archaeoglobus fulgidus Argonaute5'-end phosphorylated DNA oligoduplex, 14 bp (MZ1288) experimental SAS data
GASBOR model
Sample: Single-chain full Archaeoglobus fulgidus Argonaute monomer, 78 kDa Archaeoglobus fulgidus protein
5'-end phosphorylated DNA oligoduplex, 14 bp (MZ1288) monomer, 9 kDa DNA
Buffer: 20 mM TrisHCl pH7.5, 200 mM NaCl, 5 mM MgCl2, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 6
The missing part: the Archaeoglobus fulgidus Argonaute forms a functional heterodimer with an N-L1-L2 domain protein. Nucleic Acids Res (2024)
Manakova E, Golovinas E, Pocevičiūtė R, Sasnauskas G, Silanskas A, Rutkauskas D, Jankunec M, Zagorskaitė E, Jurgelaitis E, Grybauskas A, Venclovas Č, Zaremba M
RgGuinier 3.0 nm
Dmax 10.3 nm
VolumePorod 131 nm3

SASDRZ8 – Single-chain Archaeoglobus fulgidus argonaute fAfAgo-fusion variant complexed with an 11 base pair DNA oligoduplex in low salt buffer

single-chain full Archaeoglobus fulgidus Argonaute5'-end phosphorylated DNA guide strand, 11 nt (MZ864)DNA target strand, 11 nt (MZ865) experimental SAS data
CUSTOM IN-HOUSE model
Sample: Single-chain full Archaeoglobus fulgidus Argonaute monomer, 78 kDa Archaeoglobus fulgidus protein
5'-end phosphorylated DNA guide strand, 11 nt (MZ864) monomer, 3 kDa DNA
DNA target strand, 11 nt (MZ865) monomer, 3 kDa DNA
Buffer: 20 mM TrisHCl pH7.5, 200 mM NaCl, 5 mM MgCl2, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Aug 31
The missing part: the Archaeoglobus fulgidus Argonaute forms a functional heterodimer with an N-L1-L2 domain protein. Nucleic Acids Res (2024)
Manakova E, Golovinas E, Pocevičiūtė R, Sasnauskas G, Silanskas A, Rutkauskas D, Jankunec M, Zagorskaitė E, Jurgelaitis E, Grybauskas A, Venclovas Č, Zaremba M
RgGuinier 2.9 nm
Dmax 9.6 nm
VolumePorod 125 nm3

SASDR29 – Single-chain Archaeoglobus fulgidus argonaute fAfAgo-fusion variant in high salt buffer

single-chain full Archaeoglobus fulgidus Argonaute variant experimental SAS data
OTHER model
Sample: Single-chain full Archaeoglobus fulgidus Argonaute variant monomer, 78 kDa Archaeoglobus fulgidus protein
Buffer: 20 mM TrisHCl pH 7.5, 500 mM NaCl, 5 mM MgCl2, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Aug 31
The missing part: the Archaeoglobus fulgidus Argonaute forms a functional heterodimer with an N-L1-L2 domain protein. Nucleic Acids Res (2024)
Manakova E, Golovinas E, Pocevičiūtė R, Sasnauskas G, Silanskas A, Rutkauskas D, Jankunec M, Zagorskaitė E, Jurgelaitis E, Grybauskas A, Venclovas Č, Zaremba M
RgGuinier 3.1 nm
Dmax 9.7 nm
VolumePorod 142 nm3