Browse by ORGANISM: other species

SASDF35 – R16-24 del45-51 human dystrophin fragment

Human dystrophin central domain R16-24 del45-51 fragment experimental SAS data
Human dystrophin central domain R16-24 del45-51 fragment Kratky plot
Sample: Human dystrophin central domain R16-24 del45-51 fragment monomer, 85 kDa protein
Buffer: NaP 20 mM, NaCl 300 mM, EDTA 1 mM, Glycérol 2%, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2017 Jul 13
Dystrophin SAXS data
Raphael Dos Santos Morais
RgGuinier 7.0 nm
Dmax 26.2 nm

SASDQ59 – Mutant 7-deazapurine in DNA protein A (D95A)

DNA-guanine transglycosylase - D95A mutant experimental SAS data
GROMACS model
Sample: DNA-guanine transglycosylase - D95A mutant monomer, 50 kDa Salmonella enterica subsp. … protein
Buffer: 100 mM KCl, 50 mM Tris pH 7.0, 1 mM DTT, pH: 7
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 Dec 22
7-Deazaguanines in DNA: functional and structural elucidation of a DNA modification system. Nucleic Acids Res (2023)
Gedara SH, Wood E, Gustafson A, Liang C, Hung SH, Savage J, Phan P, Luthra A, de Crécy-Lagard V, Dedon P, Swairjo MA, Iwata-Reuyl D
RgGuinier 2.7 nm
Dmax 10.1 nm
VolumePorod 67 nm3

SASDQ69 – 28 base pair DNA substrate (used in experiments with 7-deazapurine in DNA proteins)

Custom 28 base pair double stranded DNA experimental SAS data
GROMACS model
Sample: Custom 28 base pair double stranded DNA monomer, 17 kDa Synthetic, purchased from … DNA
Buffer: 100 mM KCl, 50 mM Tris pH 7.0, 1 mM DTT, pH: 7
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 Dec 22
7-Deazaguanines in DNA: functional and structural elucidation of a DNA modification system. Nucleic Acids Res (2023)
Gedara SH, Wood E, Gustafson A, Liang C, Hung SH, Savage J, Phan P, Luthra A, de Crécy-Lagard V, Dedon P, Swairjo MA, Iwata-Reuyl D
RgGuinier 2.6 nm
Dmax 10.2 nm
VolumePorod 24 nm3

SASDQ79 – Mutant 7-deazapurine in DNA protein A (D95A) bound to a 28 base pair DNA substrate (2-state model: complex plus free DNA)

Custom 28 base pair double stranded DNADNA-guanine transglycosylase - D95A mutant experimental SAS data
GROMACS model
Sample: Custom 28 base pair double stranded DNA monomer, 17 kDa Synthetic, purchased from … DNA
DNA-guanine transglycosylase - D95A mutant monomer, 50 kDa Salmonella enterica subsp. … protein
Buffer: 100 mM KCl, 50 mM Tris pH 7.0, 1 mM DTT, pH: 7
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 Dec 22
7-Deazaguanines in DNA: functional and structural elucidation of a DNA modification system. Nucleic Acids Res (2023)
Gedara SH, Wood E, Gustafson A, Liang C, Hung SH, Savage J, Phan P, Luthra A, de Crécy-Lagard V, Dedon P, Swairjo MA, Iwata-Reuyl D
RgGuinier 3.4 nm
Dmax 13.5 nm
VolumePorod 104 nm3

SASDQ89 – 7-deazapurine in DNA protein B

DGQHR domain-containing protein experimental SAS data
ALPHAFOLD model
Sample: DGQHR domain-containing protein dimer, 88 kDa Salmonella enterica subsp. … protein
Buffer: 100 mM KCl, 50 mM Tris pH 7.0, 1 mM DTT, pH: 7
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 Dec 22
7-Deazaguanines in DNA: functional and structural elucidation of a DNA modification system. Nucleic Acids Res (2023)
Gedara SH, Wood E, Gustafson A, Liang C, Hung SH, Savage J, Phan P, Luthra A, de Crécy-Lagard V, Dedon P, Swairjo MA, Iwata-Reuyl D
RgGuinier 3.4 nm
Dmax 13.5 nm
VolumePorod 126 nm3

SASDPF5 – SAXS curve of RNA Xist A-repeats (14mer)

Xist A-repeat lncRNA 14 mer experimental SAS data
Xist A-repeat lncRNA 14 mer Kratky plot
Sample: Xist A-repeat lncRNA 14 mer monomer, 5 kDa RNA
Buffer: 50 mM HEPES, 150 mM NaCl, 2 mM MgCl2, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 20
Cotranscriptional folding of the lncRNA Xist A-repeats indicates a modular structure
Alisha Jones
RgGuinier 2.1 nm
Dmax 8.5 nm
VolumePorod 13 nm3

SASDPG5 – SAXS curve of RNA Xist A-repeats (26mer)

Xist A-repeat lncRNA 24 mer experimental SAS data
Xist A-repeat lncRNA 24 mer Kratky plot
Sample: Xist A-repeat lncRNA 24 mer dimer, 16 kDa RNA
Buffer: 50 mM HEPES, 150 mM NaCl, 2 mM MgCl2, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 20
Cotranscriptional folding of the lncRNA Xist A-repeats indicates a modular structure
Alisha Jones
RgGuinier 3.1 nm
Dmax 14.5 nm
VolumePorod 28 nm3

SASDPH5 – SAXS curve of RNA Xist A-repeats (1 repeat)

Xist A-repeat lncRNA 1 repeat experimental SAS data
Xist A-repeat lncRNA 1 repeat Kratky plot
Sample: Xist A-repeat lncRNA 1 repeat dimer, 41 kDa RNA
Buffer: 50 mM HEPES, 150 mM NaCl, 2 mM MgCl2, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 20
Cotranscriptional folding of the lncRNA Xist A-repeats indicates a modular structure
Alisha Jones
RgGuinier 3.9 nm
Dmax 16.0 nm
VolumePorod 42 nm3

SASDPJ5 – SAXS curve of RNA Xist A-repeats (2 repeats)

Xist A-repeat lncRNA 2 repeats experimental SAS data
Xist A-repeat lncRNA 2 repeats Kratky plot
Sample: Xist A-repeat lncRNA 2 repeats monomer, 34 kDa RNA
Buffer: 50 mM HEPES, 150 mM NaCl, 2 mM MgCl2, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 20
Cotranscriptional folding of the lncRNA Xist A-repeats indicates a modular structure
Alisha Jones
RgGuinier 5.0 nm
Dmax 23.0 nm
VolumePorod 87 nm3

SASDPK5 – SAXS curve of RNA Xist A-repeats (3 repeats)

Xist A-repeat lncRNA 3 repeats experimental SAS data
Xist A-repeat lncRNA 3 repeats Kratky plot
Sample: Xist A-repeat lncRNA 3 repeats monomer, 48 kDa RNA
Buffer: 50 mM HEPES, 150 mM NaCl, 2 mM MgCl2, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 20
Cotranscriptional folding of the lncRNA Xist A-repeats indicates a modular structure
Alisha Jones
RgGuinier 6.3 nm
Dmax 22.0 nm
VolumePorod 97 nm3