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SASDSY6 – Outer membrane protein MIP (LpMIP) apo state

Outer membrane protein MIP experimental SAS data
SREFLEX model
Sample: Outer membrane protein MIP dimer, 46 kDa Legionella pneumophila subsp. … protein
Buffer: 20 mM Tris-HCl, 150 mM NaCl, 10 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 16
Legionella pneumophila macrophage infectivity potentiator protein appendage domains modulate protein dynamics and inhibitor binding International Journal of Biological Macromolecules :126366 (2023)
Wiedemann C, Whittaker J, Carrillo V, Goretzki B, Dajka M, Tebbe F, Harder J, Krajczy P, Joseph B, Hausch F, Guskov A, Hellmich U
RgGuinier 3.1 nm
Dmax 10.0 nm
VolumePorod 66 nm3

SASDSZ6 – Outer membrane protein MIP (LpMIP) with bound inhibitor JK095

Outer membrane protein MIP experimental SAS data
SREFLEX model
Sample: Outer membrane protein MIP dimer, 46 kDa Legionella pneumophila subsp. … protein
Buffer: 20 mM Tris-HCl, 150 mM NaCl, 10 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 16
Legionella pneumophila macrophage infectivity potentiator protein appendage domains modulate protein dynamics and inhibitor binding International Journal of Biological Macromolecules :126366 (2023)
Wiedemann C, Whittaker J, Carrillo V, Goretzki B, Dajka M, Tebbe F, Harder J, Krajczy P, Joseph B, Hausch F, Guskov A, Hellmich U
RgGuinier 3.1 nm
Dmax 10.2 nm
VolumePorod 65 nm3

SASDS27 – Outer membrane protein MIP (LpMIP) with bound inhibitor JK236

Outer membrane protein MIP experimental SAS data
SREFLEX model
Sample: Outer membrane protein MIP dimer, 46 kDa Legionella pneumophila subsp. … protein
Buffer: 20 mM Tris-HCl, 150 mM NaCl, 10 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 16
Legionella pneumophila macrophage infectivity potentiator protein appendage domains modulate protein dynamics and inhibitor binding International Journal of Biological Macromolecules :126366 (2023)
Wiedemann C, Whittaker J, Carrillo V, Goretzki B, Dajka M, Tebbe F, Harder J, Krajczy P, Joseph B, Hausch F, Guskov A, Hellmich U
RgGuinier 3.1 nm
Dmax 9.4 nm
VolumePorod 63 nm3

SASDPF5 – SAXS curve of RNA Xist A-repeats (14mer)

Xist A-repeat lncRNA 14 mer experimental SAS data
Xist A-repeat lncRNA 14 mer Kratky plot
Sample: Xist A-repeat lncRNA 14 mer monomer, 5 kDa RNA
Buffer: 50 mM HEPES, 150 mM NaCl, 2 mM MgCl2, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 20
Conformation and structural dynamics of the Xist lncRNA A-repeats (2023)
Jones A, Gabel F, Bohn S, Wolfe G, Sattler M
RgGuinier 2.1 nm
Dmax 8.5 nm
VolumePorod 13 nm3

SASDPG5 – SAXS curve of RNA Xist A-repeats (26mer)

Xist A-repeat lncRNA 24 mer experimental SAS data
Xist A-repeat lncRNA 24 mer Kratky plot
Sample: Xist A-repeat lncRNA 24 mer dimer, 16 kDa RNA
Buffer: 50 mM HEPES, 150 mM NaCl, 2 mM MgCl2, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 20
Conformation and structural dynamics of the Xist lncRNA A-repeats (2023)
Jones A, Gabel F, Bohn S, Wolfe G, Sattler M
RgGuinier 3.1 nm
Dmax 14.5 nm
VolumePorod 28 nm3

SASDPH5 – SAXS curve of RNA Xist A-repeats (1 repeat)

Xist A-repeat lncRNA 1 repeat experimental SAS data
Xist A-repeat lncRNA 1 repeat Kratky plot
Sample: Xist A-repeat lncRNA 1 repeat dimer, 41 kDa RNA
Buffer: 50 mM HEPES, 150 mM NaCl, 2 mM MgCl2, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 20
Conformation and structural dynamics of the Xist lncRNA A-repeats (2023)
Jones A, Gabel F, Bohn S, Wolfe G, Sattler M
RgGuinier 3.9 nm
Dmax 16.0 nm
VolumePorod 42 nm3

SASDPJ5 – SAXS curve of RNA Xist A-repeats (2 repeats)

Xist A-repeat lncRNA 2 repeats experimental SAS data
Xist A-repeat lncRNA 2 repeats Kratky plot
Sample: Xist A-repeat lncRNA 2 repeats monomer, 34 kDa RNA
Buffer: 50 mM HEPES, 150 mM NaCl, 2 mM MgCl2, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 20
Conformation and structural dynamics of the Xist lncRNA A-repeats (2023)
Jones A, Gabel F, Bohn S, Wolfe G, Sattler M
RgGuinier 5.0 nm
Dmax 23.0 nm
VolumePorod 87 nm3

SASDPK5 – SAXS curve of RNA Xist A-repeats (3 repeats)

Xist A-repeat lncRNA 3 repeats experimental SAS data
Xist A-repeat lncRNA 3 repeats Kratky plot
Sample: Xist A-repeat lncRNA 3 repeats monomer, 48 kDa RNA
Buffer: 50 mM HEPES, 150 mM NaCl, 2 mM MgCl2, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 20
Conformation and structural dynamics of the Xist lncRNA A-repeats (2023)
Jones A, Gabel F, Bohn S, Wolfe G, Sattler M
RgGuinier 6.3 nm
Dmax 22.0 nm
VolumePorod 97 nm3

SASDPL5 – SAXS curve of RNA Xist A-repeats (4 repeats)

Xist A-repeat lncRNA 4 repeats experimental SAS data
Xist A-repeat lncRNA 4 repeats Kratky plot
Sample: Xist A-repeat lncRNA 4 repeats monomer, 62 kDa RNA
Buffer: 50 mM HEPES, 150 mM NaCl, 2 mM MgCl2, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 20
Conformation and structural dynamics of the Xist lncRNA A-repeats (2023)
Jones A, Gabel F, Bohn S, Wolfe G, Sattler M
RgGuinier 7.2 nm
Dmax 34.0 nm

SASDPM5 – SAXS curve of RNA Xist A-repeats (5 repeats)

Xist A-repeat lncRNA 5 repeats experimental SAS data
Xist A-repeat lncRNA 5 repeats Kratky plot
Sample: Xist A-repeat lncRNA 5 repeats monomer, 81 kDa RNA
Buffer: 50 mM HEPES, 150 mM NaCl, 2 mM MgCl2, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 20
Conformation and structural dynamics of the Xist lncRNA A-repeats (2023)
Jones A, Gabel F, Bohn S, Wolfe G, Sattler M
RgGuinier 7.7 nm
Dmax 30.0 nm
VolumePorod 186 nm3