Browse by ORGANISM: other species

SASDN42 – Dockerin domain-containing protein, starch adherence system 20 (Sas20), domains 1 and 2 with maltoheptaose

Dockerin domain-containing protein experimental SAS data
Dockerin domain-containing protein Kratky plot
Sample: Dockerin domain-containing protein monomer, 26 kDa Ruminococcus bromii protein
Buffer: phosphate buffered saline, 1 mM TCEP, pH: 7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 14
Sas20 is a highly flexible starch-binding protein in the Ruminococcus bromii cell-surface amylosome Journal of Biological Chemistry :101896 (2022)
Cerqueira F, Photenhauer A, Doden H, Brown A, Abdel-Hamid A, Moraïs S, Bayer E, Wawrzak Z, Cann I, Ridlon J, Hopkins J, Koropatkin N
RgGuinier 5.2 nm
Dmax 19.0 nm
VolumePorod 84 nm3

SASDKY9 – A minimal intact methyltransferase of a type I restriction-modification system

Type I restriction-modification system methyltransferase subunitProtein Ocr experimental SAS data
DAMMIF model
Sample: Type I restriction-modification system methyltransferase subunit dimer, 144 kDa Vibrio vulnificus (strain … protein
Protein Ocr dimer, 28 kDa Escherichia phage T7 protein
Buffer: 20 mM Tris-HCl,150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 4C, Pohang Accelerator Laboratory on 2020 Apr 22
Structural features of a minimal intact methyltransferase of a type I restriction-modification system. Int J Biol Macromol (2022)
Seo PW, Hofmann A, Kim JH, Hwangbo SA, Kim JH, Kim JW, Huynh TYL, Choy HE, Kim SJ, Lee J, Lee JO, Jin KS, Park SY, Kim JS
RgGuinier 4.2 nm
Dmax 15.9 nm
VolumePorod 354 nm3

SASDNJ2 – Circadian clock KaiA-KaiB-KaiC ternary protein complex measured by SEC-SAXS

Circadian clock protein KaiACircadian clock protein KaiBCircadian clock protein kinase KaiC (S431D mutant) experimental SAS data
OTHER model
Sample: Circadian clock protein KaiA dodecamer, 393 kDa Synechococcus elongatus (strain … protein
Circadian clock protein KaiB hexamer, 71 kDa Synechococcus elongatus (strain … protein
Circadian clock protein kinase KaiC (S431D mutant) hexamer, 357 kDa Synechococcus elongatus (strain … protein
Buffer: 50 mM sodium phosphate buffer, 150 mM NaCl, 5 mM MgCl2, 0.5 mM EDTA, 1 mM ATP, 1 mM DTT, 50 mM arginine, 50 mM glutamine, pH: 7.8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2017 Dec 8
Overall structure of fully assembled cyanobacterial KaiABC circadian clock complex by an integrated experimental-computational approach. Commun Biol 5(1):184 (2022)
Yunoki Y, Matsumoto A, Morishima K, Martel A, Porcar L, Sato N, Yogo R, Tominaga T, Inoue R, Yagi-Utsumi M, Okuda A, Shimizu M, Urade R, Terauchi K, Kono H, Yagi H, Kato K, Sugiyama M
RgGuinier 7.0 nm
Dmax 24.5 nm
VolumePorod 1650 nm3

SASDNK2 – Circadian clock hKaiA-75dKaiB-75dKaiC ternary protein complex measured by SEC-SANS in 100% D2O

75% deuterated Circadian clock protein KaiBCircadian clock protein KaiA75% deuterated Circadian clock protein kinase KaiC (S431D mutant) experimental SAS data
OTHER model
Sample: 75% deuterated Circadian clock protein KaiB hexamer, 71 kDa Synechococcus elongatus (strain … protein
Circadian clock protein KaiA dodecamer, 393 kDa Synechococcus elongatus (strain … protein
75% deuterated Circadian clock protein kinase KaiC (S431D mutant) hexamer, 357 kDa Synechococcus elongatus (strain … protein
Buffer: 50 mM sodium phosphate buffer, 150 mm NaCl, 5 mM MgCl2, 0.5 mM EDTA, 1 mM ATP, 1 mM DTT, 50 mM arginine, 50 mM glutamine, in 100% D2O, pH: 7.8
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2018 Sep 19
Overall structure of fully assembled cyanobacterial KaiABC circadian clock complex by an integrated experimental-computational approach. Commun Biol 5(1):184 (2022)
Yunoki Y, Matsumoto A, Morishima K, Martel A, Porcar L, Sato N, Yogo R, Tominaga T, Inoue R, Yagi-Utsumi M, Okuda A, Shimizu M, Urade R, Terauchi K, Kono H, Yagi H, Kato K, Sugiyama M
RgGuinier 7.8 nm
Dmax 25.6 nm
VolumePorod 1620 nm3

SASDLE9 – Suppressor of Copper Sensitivity C protein from Caulobacter crescentus

Thioredoxin domain-containing protein experimental SAS data
Suppressor of Copper Sensitivity C protein from Caulobacter crescentus Rg histogram
Sample: Thioredoxin domain-containing protein trimer, 73 kDa Caulobacter vibrioides (strain … protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Aug 21
The suppressor of copper sensitivity protein C from Caulobacter crescentus is a trimeric disulfide isomerase that binds copper(I) with subpicomolar affinity Acta Crystallographica Section D Structural Biology 78(3):337-352 (2022)
Petit G, Hong Y, Djoko K, Whitten A, Furlong E, McCoy A, Gulbis J, Totsika M, Martin J, Halili M
RgGuinier 3.9 nm
Dmax 12.0 nm
VolumePorod 97 nm3

SASDKY7 – α-L-Fucosidase isoenzyme 2 from Paenibacillus thiaminolyticus - wild type

Alpha-L-fucosidase experimental SAS data
OTHER model
Sample: Alpha-L-fucosidase tetramer, 297 kDa Paenibacillus thiaminolyticus (Bacillus … protein
Buffer: 50 mM potassium phosphate, pH: 7.4
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2019 Jul 16
The first structure–function study of GH151 α‐ l ‐fucosidase uncovers new oligomerization pattern, active site complementation, and selective substrate specificity The FEBS Journal (2022)
Koval'ová T, Kovaľ T, Stránský J, Kolenko P, Dušková J, Švecová L, Vodičková P, Spiwok V, Benešová E, Lipovová P, Dohnálek J
RgGuinier 4.2 nm
Dmax 13.2 nm
VolumePorod 418 nm3

SASDKZ7 – α-L-Fucosidase isoenzyme 2 from Paenibacillus thiaminolyticus - mutant H503A

Alpha-L-fucosidase H503A experimental SAS data
PYMOL model
Sample: Alpha-L-fucosidase H503A tetramer, 297 kDa Paenibacillus thiaminolyticus (Bacillus … protein
Buffer: 50 mM potassium phosphate, pH: 7.4
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2019 Jul 16
The first structure–function study of GH151 α‐ l ‐fucosidase uncovers new oligomerization pattern, active site complementation, and selective substrate specificity The FEBS Journal (2022)
Koval'ová T, Kovaľ T, Stránský J, Kolenko P, Dušková J, Švecová L, Vodičková P, Spiwok V, Benešová E, Lipovová P, Dohnálek J
RgGuinier 4.5 nm
Dmax 13.5 nm
VolumePorod 433 nm3

SASDPL8 – Molecular brush (PiPOx239-g-PnPrOx14) in a good solvent at concentration c=1.25 mg/mL

PiPOx239-g-PnPrOx14 experimental SAS data
DAMMIF model
Sample: PiPOx239-g-PnPrOx14 monomer, 413 kDa
Buffer: ethanol, pH: 7.3
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Jun 12
Rigid-to-Flexible Transition in a Molecular Brush in a Good Solvent at a Semidilute Concentration Langmuir 38(17):5226-5236 (2022)
Kang J, Sachse C, Ko C, Schroer M, Vela S, Molodenskiy D, Kohlbrecher J, Bushuev N, Gumerov R, Potemkin I, Jordan R, Papadakis C
RgGuinier 11.2 nm
Dmax 37.6 nm

SASDPM8 – Molecular brush (PiPOx239-g-PnPrOx14) in a good solvent at concentration c=10 mg/mL

PiPOx239-g-PnPrOx14 experimental SAS data
DAMMIF model
Sample: PiPOx239-g-PnPrOx14 monomer, 413 kDa
Buffer: ethanol, pH: 7.3
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Jun 12
Rigid-to-Flexible Transition in a Molecular Brush in a Good Solvent at a Semidilute Concentration Langmuir 38(17):5226-5236 (2022)
Kang J, Sachse C, Ko C, Schroer M, Vela S, Molodenskiy D, Kohlbrecher J, Bushuev N, Gumerov R, Potemkin I, Jordan R, Papadakis C
RgGuinier 8.6 nm
Dmax 34.3 nm

SASDPN8 – Molecular brush (PiPOx239-g-PnPrOx14) in a good solvent at concentration c=46 mg/mL

PiPOx239-g-PnPrOx14 experimental SAS data
DAMMIF model
Sample: PiPOx239-g-PnPrOx14 monomer, 413 kDa
Buffer: ethanol, pH: 7.3
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Jun 12
Rigid-to-Flexible Transition in a Molecular Brush in a Good Solvent at a Semidilute Concentration Langmuir 38(17):5226-5236 (2022)
Kang J, Sachse C, Ko C, Schroer M, Vela S, Molodenskiy D, Kohlbrecher J, Bushuev N, Gumerov R, Potemkin I, Jordan R, Papadakis C
RgGuinier 7.2 nm
Dmax 18.5 nm