Small Angle Scattering Biological Data Bank SASBDB

Browse by ORGANISM: other species

SASDNK3 – HIV-1 reverse transcriptase L289K mutation at 4.3 mg/mL

Gag-Pol polyprotein (HIV-1 reverse transcriptase C38V/C280S, L289K truncated to 1 - 556) experimental SAS data

Gag-Pol polyprotein (HIV-1 reverse transcriptase C38V/C280S, L289K truncated to 1 - 556) Kratky plot

Sample:	Gag-Pol polyprotein (HIV-1 reverse transcriptase C38V/C280S, L289K truncated to 1 - 556) monomer, 65 kDa Human immunodeficiency virus … protein
Buffer:	50 mM Bis-Tris pH 6.8, 100 mM NaCl, 1% glycerol, 0.02% w/v NaN3, pH: 6.8
Experiment:	SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Jun 20

Relative domain orientation of the L289K HIV ‐1 reverse transcriptase monomer Protein Science 31(5) (2022)
Xi Z, Ilina T, Guerrero M, Fan L, Sluis‐Cremer N, Wang Y, Ishima R

R_g^Guinier	3.2	nm
D_max	10.7	nm
Volume^Porod	93	nm³

SASDNL3 – HIV-1 reverse transcriptase L289K mutation (extrapolated to zero concentration)

Sample:	Gag-Pol polyprotein (HIV-1 reverse transcriptase C38V/C280S, L289K truncated to 1 - 556) monomer, 65 kDa Human immunodeficiency virus … protein
Buffer:	50 mM Bis-Tris pH 6.8, 100 mM NaCl, 1% glycerol, 0.02% w/v NaN3, pH: 6.8
Experiment:	SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Jun 20

Relative domain orientation of the L289K HIV ‐1 reverse transcriptase monomer Protein Science 31(5) (2022)
Xi Z, Ilina T, Guerrero M, Fan L, Sluis‐Cremer N, Wang Y, Ishima R

R_g^Guinier	3.2	nm
D_max	11.1	nm
Volume^Porod	90	nm³

SASDMX5 – Zea mays beta-amylase (ZmBAM7) short

Sample:	Beta-amylase tetramer, 231 kDa Zea mays protein
Buffer:	50 mM HEPES, 25 mM NaCl, and 0.2 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Dec 7

The BAM7 gene in Zea mays encodes a protein with similar structural and catalytic properties to Arabidopsis BAM2 Acta Crystallographica Section D Structural Biology 78(5) (2022)
Ravenburg C, Riney M, Monroe J, Berndsen C

R_g^Guinier	5.3	nm
D_max	16.3	nm
Volume^Porod	615	nm³

SASDMY5 – Truncated Zea mays beta-amylase (ZmBAM7) short

Sample:	Beta-amylase tetramer, 231 kDa Zea mays protein
Buffer:	50 mM HEPES, 25 mM NaCl, and 0.2 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Dec 7

R_g^Guinier	5.1	nm
D_max	13.9	nm
Volume^Porod	467	nm³

SASDM26 – Sweet potato beta-amylase 5

Sample:	Beta-amylase tetramer, 224 kDa Ipomoea batatas protein
Buffer:	20 mM HEPES, 150 mM NaCl, and 0.2 mM TCEP, pH: 7.3
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Dec 7

R_g^Guinier	4.4	nm
D_max	14.1	nm
Volume^Porod	296	nm³

SASDMX9 – Dockerin domain-containing protein, starch adherence system 20 (Sas20), domain 1

Dockerin domain-containing protein experimental SAS data

Sample:	Dockerin domain-containing protein monomer, 26 kDa Ruminococcus bromii protein
Buffer:	phosphate buffered saline, 1 mM TCEP, pH: 7
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Mar 28

Sas20 is a highly flexible starch-binding protein in the Ruminococcus bromii cell-surface amylosome Journal of Biological Chemistry :101896 (2022)
Cerqueira F, Photenhauer A, Doden H, Brown A, Abdel-Hamid A, Moraïs S, Bayer E, Wawrzak Z, Cann I, Ridlon J, Hopkins J, Koropatkin N

R_g^Guinier	2.1	nm
D_max	10.3	nm
Volume^Porod	42	nm³

SASDMY9 – Dockerin domain-containing protein, starch adherence system 20 (Sas20), domain 2

Sample:	Dockerin domain-containing protein monomer, 26 kDa Ruminococcus bromii protein
Buffer:	phosphate buffered saline, 1 mM TCEP, pH: 7
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Mar 28

R_g^Guinier	2.3	nm
D_max	7.8	nm
Volume^Porod	35	nm³

SASDMZ9 – Dockerin domain-containing protein, starch adherence system 20 (Sas20), domains 1 and 2

Sample:	Dockerin domain-containing protein monomer, 57 kDa Ruminococcus bromii protein
Buffer:	phosphate buffered saline, 1 mM TCEP, pH: 7
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Mar 28

R_g^Guinier	5.4	nm
D_max	20.3	nm
Volume^Porod	93	nm³

SASDN22 – Dockerin domain-containing protein, starch adherence system 20 (Sas20), domain 1 with maltoheptaose

Dockerin domain-containing protein Kratky plot

Sample:	Dockerin domain-containing protein monomer, 26 kDa Ruminococcus bromii protein
Buffer:	phosphate buffered saline, 1 mM TCEP, pH: 7
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 14

R_g^Guinier	2.0	nm
D_max	7.8	nm
Volume^Porod	45	nm³

SASDN32 – Dockerin domain-containing protein, starch adherence system 20 (Sas20), domain 2 with maltoheptaose

Sample:	Dockerin domain-containing protein monomer, 26 kDa Ruminococcus bromii protein
Buffer:	phosphate buffered saline, 1 mM TCEP, pH: 7
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 14

R_g^Guinier	2.1	nm
D_max	7.4	nm
Volume^Porod	41	nm³

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