Small Angle Scattering Biological Data Bank SASBDB

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SASDKZ5 – RNA Long Tetraloop Hairpin Triplex in 200 mM KCl

RNA Long Tetraloop Hairpin Triplex experimental SAS data

RNA Long Tetraloop Hairpin Triplex Kratky plot

Sample:	RNA Long Tetraloop Hairpin Triplex monomer, 28 kDa RNA
Buffer:	200 mM KCl, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment:	SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20

RNA triplex structures revealed by WAXS-driven MD simulations (2022)
Chen Y, He W, Kirmizialtin S, Pollack L

R_g^Guinier	2.8	nm
D_max	10.3	nm

SASDK26 – RNA Long Tetraloop Hairpin Triplex in 5 mM MgCl2

Sample:	RNA Long Tetraloop Hairpin Triplex monomer, 28 kDa RNA
Buffer:	5 mM MgCl2, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment:	SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20

RNA triplex structures revealed by WAXS-driven MD simulations (2022)
Chen Y, He W, Kirmizialtin S, Pollack L

R_g^Guinier	2.9	nm
D_max	18.2	nm

SASDL87 – Plasmodium falciparum Glycogen Synthase Kinase 3 - Ion Exchange Chromatography Fraction 4

Glycogen synthase kinase 3 experimental SAS data

Sample:	Glycogen synthase kinase 3 monomer, 52 kDa Plasmodium falciparum (isolate … protein
Buffer:	20 mM Tris pH 8.0, 100 mM NaCl, 0.5 mM TCEP, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Nov 19

N-terminal phosphorylation regulates the activity of glycogen synthase kinase 3 from Plasmodium falciparum. Biochem J 479(3):337-356 (2022)
Pazicky S, Alder A, Mertens H, Svergun D, Gilberger T, Löw C

R_g^Guinier	3.2	nm
D_max	11.6	nm
Volume^Porod	101	nm³

SASDMC9 – Plasmodium falciparum Glycogen Synthase Kinase 3 - Ion Exchange Chromatography Fraction 1

Sample:	Glycogen synthase kinase 3 monomer, 52 kDa Plasmodium falciparum (isolate … protein
Buffer:	20 mM Tris pH 8.0, 100 mM NaCl, 0.5 mM TCEP, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Nov 19

R_g^Guinier	3.3	nm
D_max	13.0	nm
Volume^Porod	102	nm³

SASDJV3 – GTPase-Activation Protein (GAP) domain (73-204) of Vibrio cholerae Outer Protein E (VopE)

Outer membrane virulence protein yopE experimental SAS data

Outer membrane virulence protein yopE Kratky plot

Sample:	Outer membrane virulence protein yopE monomer, 15 kDa Vibrio cholerae protein
Buffer:	10 mM HEPES-HCl, pH 7.4, 150 mM NaCl, 0.5 mM TCEP, pH: 7.4
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2016 Jun 29

Solution structure and dynamics of the mitochondrial‐targeted GTPase ‐activating protein ( GAP ) VopE by an integrated NMR / SAXS approach Protein Science (2022)
Smith K, Lee W, Tonelli M, Lee Y, Light S, Cornilescu G, Chakravarthy S

R_g^Guinier	2.0	nm
D_max	8.0	nm
Volume^Porod	28	nm³

SASDM42 – Cobalt/magnesium transport protein CorA in matched-out deuterated dodecylmaltoside (dDDM) micelles without Mg2+

Cobalt/magnesium transport protein CorA experimental SAS data

Cobalt/magnesium transport protein CorA Kratky plot

Sample:	Cobalt/magnesium transport protein CorA pentamer, 208 kDa Thermotoga maritima (strain … protein
Buffer:	20 mM Tris-DCl, 150 mM NaCl, 1 mM EDTA-NaOD, in 100% D2O, pH: 7.5
Experiment:	SANS data collected at D22, Institut Laue-Langevin (ILL) on 2018 Jun 23

Mg²⁺-dependent conformational equilibria in CorA and an integrated view on transport regulation. Elife 11 (2022)
Johansen NT, Bonaccorsi M, Bengtsen T, Larsen AH, Tidemand FG, Pedersen MC, Huda P, Berndtsson J, Darwish T, Yepuri NR, Martel A, Pomorski TG, Bertarello A, Sansom M, Rapp M, Crehuet R, Schubeis T, Lindorff-Larsen K, Pintacuda G, Arleth L

R_g^Guinier	4.2	nm
D_max	13.9	nm

SASDM52 – Cobalt/magnesium transport protein CorA in matched-out deuterated dodecylmaltoside (dDDM) micelles with bound Mg2+

Sample:	Cobalt/magnesium transport protein CorA pentamer, 208 kDa Thermotoga maritima (strain … protein
Buffer:	20 mM Tris-DCl, 150 mM NaCl, 40 mM MgCl2, in 100% D2O, pH: 7.5
Experiment:	SANS data collected at D22, Institut Laue-Langevin (ILL) on 2018 Jun 23

R_g^Guinier	4.3	nm
D_max	13.9	nm

SASDM62 – Cobalt/magnesium transport protein CorA in matched-out deuterated nanodiscs without Mg2+

Sample:	Cobalt/magnesium transport protein CorA pentamer, 208 kDa Thermotoga maritima (strain … protein
Buffer:	20 mM Tris-DCl, 150 mM NaCl, 1 mM EDTA-NaOD, in 100% D2O, pH: 7.5
Experiment:	SANS data collected at D22, Institut Laue-Langevin (ILL) on 2018 Jun 23

R_g^Guinier	4.8	nm
D_max	14.2	nm

SASDM72 – Cobalt/magnesium transport protein CorA in match-out deuterated nanodiscs with bound Mg2+

Sample:	Cobalt/magnesium transport protein CorA pentamer, 208 kDa Thermotoga maritima (strain … protein
Buffer:	20 mM Tris-DCl, 150 mM NaCl, 40 mM MgCl2, in 100% D2O, pH: 7.5
Experiment:	SANS data collected at D22, Institut Laue-Langevin (ILL) on 2018 Jun 23

R_g^Guinier	4.9	nm
D_max	14.3	nm

SASDMM4 – Hepatitis C virus 5BSL3.2 RNA subdomain

Sample:	5BSL3.2 monomer, 15 kDa Hepatitis C virus … RNA
Buffer:	10 mM Tris-HCl, 0.1 mM EDTA, pH: 7
Experiment:	SAXS data collected at Rigaku BioSAXS-1000, NIDDK, NIH on 2019 Jun 2

The low-resolution structural models of hepatitis C virus RNA subdomain 5BSL3.2 and its distal complex with domain 3'X point to conserved regulatory mechanisms within the Flaviviridae family. Nucleic Acids Res (2022)
Castillo-Martínez J, Fan L, Szewczyk MP, Wang YX, Gallego J

R_g^Guinier	2.5	nm
D_max	7.6	nm
Volume^Porod	29	nm³

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