SASDSA7 – polymerase alpha-primase polΔcat-prim bound to DNA in the presence of an RNA-primed template

DNA polymerase alpha subunit BDNA polymerase alpha catalytic subunitDNA primase large subunitDNA primase small subunit29 mer DNA template8mer RNA primer experimental SAS data
DNA polymerase alpha subunit B DNA polymerase alpha catalytic subunit DNA primase large subunit DNA primase small subunit 29 mer DNA template 8mer RNA primer Kratky plot
Sample: DNA polymerase alpha subunit B monomer, 49 kDa Homo sapiens protein
DNA polymerase alpha catalytic subunit monomer, 23 kDa Homo sapiens protein
DNA primase large subunit monomer, 59 kDa Homo sapiens protein
DNA primase small subunit monomer, 50 kDa Homo sapiens protein
29 mer DNA template monomer, 9 kDa DNA
8mer RNA primer monomer, 3 kDa RNA
Buffer: 20 mM HEPES, 150 mM NaCl, 5 mM MgCl2, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 Mar 28
Flexibility and distributive synthesis regulate RNA priming and handoff in human DNA polymerase α-primase Journal of Molecular Biology :168330 (2023)
Cordoba J, Mullins E, Salay L, Eichman B, Chazin W
RgGuinier 4.9 nm
Dmax 15.3 nm
VolumePorod 344 nm3

SASDSB7 – polymerase alpha-primase polΔcat-primΔ2C in the absence of substrate

DNA polymerase alpha subunit BDNA polymerase alpha catalytic subunitDNA primase small subunitDNA primase large subunit experimental SAS data
DNA polymerase alpha subunit B DNA polymerase alpha catalytic subunit DNA primase small subunit DNA primase large subunit Kratky plot
Sample: DNA polymerase alpha subunit B monomer, 49 kDa Homo sapiens protein
DNA polymerase alpha catalytic subunit monomer, 23 kDa Homo sapiens protein
DNA primase small subunit monomer, 50 kDa Homo sapiens protein
DNA primase large subunit monomer, 31 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5 mM MgCl2, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Apr 15
Flexibility and distributive synthesis regulate RNA priming and handoff in human DNA polymerase α-primase Journal of Molecular Biology :168330 (2023)
Cordoba J, Mullins E, Salay L, Eichman B, Chazin W
RgGuinier 4.5 nm
Dmax 14.5 nm
VolumePorod 221 nm3

SASDK86 – wild-type TRIM72 (Tripartite motif-containing protein 72)

Tripartite motif-containing protein 72 experimental SAS data
DAMMIN model
Sample: Tripartite motif-containing protein 72 dimer, 105 kDa Mus musculus protein
Buffer: 25 mM Tris-HCl, pH 8.0, 300 mM NaCl, 1 mM TCEP, 1 mM DTT, pH: 8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2019 Dec 7
Structure and activation of the RING E3 ubiquitin ligase TRIM72 on the membrane. Nat Struct Mol Biol (2023)
Park SH, Han J, Jeong BC, Song JH, Jang SH, Jeong H, Kim BH, Ko YG, Park ZY, Lee KE, Hyun J, Song HK
RgGuinier 6.9 nm
Dmax 24.0 nm
VolumePorod 384 nm3

SASDK96 – tripartite motif-containing protein 72 (TRIM72) lacking the zinc finger RING domain (ΔRING)

Tripartite motif-containing protein 72 experimental SAS data
OTHER model
Sample: Tripartite motif-containing protein 72 dimer, 88 kDa Mus musculus protein
Buffer: 25 mM Tris-HCl, pH 8.0, 300 mM NaCl, 1 mM TCEP, 1 mM DTT, pH: 8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2019 Dec 7
Structure and activation of the RING E3 ubiquitin ligase TRIM72 on the membrane. Nat Struct Mol Biol (2023)
Park SH, Han J, Jeong BC, Song JH, Jang SH, Jeong H, Kim BH, Ko YG, Park ZY, Lee KE, Hyun J, Song HK
RgGuinier 5.1 nm
Dmax 18.7 nm
VolumePorod 142 nm3

SASDR25 – Periplasmic domain of cholera toxin transcriptional activator ToxR

Cholera toxin transcriptional activator experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Cholera toxin transcriptional activator monomer, 12 kDa Vibrio cholerae serotype … protein
Buffer: 50 mM Na2HPO4, 300 mM NaCl, 3% glycerol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Mar 6
Vibrio cholerae's ToxRS bile sensing system. Elife 12 (2023)
Gubensäk N, Sagmeister T, Buhlheller C, Geronimo BD, Wagner GE, Petrowitsch L, Gräwert MA, Rotzinger M, Berger TMI, Schäfer J, Usón I, Reidl J, Sánchez-Murcia PA, Zangger K, Pavkov-Keller T
RgGuinier 1.6 nm
Dmax 6.0 nm
VolumePorod 16 nm3

SASDR35 – Periplasmic domain of cholera transmembrane regulatory protein ToxS

Transmembrane regulatory protein ToxS experimental SAS data
ALPHAFOLD model
Sample: Transmembrane regulatory protein ToxS dimer, 37 kDa Vibrio cholerae serotype … protein
Buffer: 50 mM Na2HPO4, 300 mM NaCl, 3% glycerol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Mar 6
Vibrio cholerae's ToxRS bile sensing system. Elife 12 (2023)
Gubensäk N, Sagmeister T, Buhlheller C, Geronimo BD, Wagner GE, Petrowitsch L, Gräwert MA, Rotzinger M, Berger TMI, Schäfer J, Usón I, Reidl J, Sánchez-Murcia PA, Zangger K, Pavkov-Keller T
RgGuinier 2.3 nm
Dmax 7.4 nm
VolumePorod 55 nm3

SASDR45 – A complex between the periplasmic domains of cholera toxin transcriptional activator ToxR and transmembrane regulatory protein ToxS

Cholera toxin transcriptional activatorTransmembrane regulatory protein ToxS experimental SAS data
CUSTOM IN-HOUSE model
Sample: Cholera toxin transcriptional activator monomer, 12 kDa Vibrio cholerae serotype … protein
Transmembrane regulatory protein ToxS dimer, 37 kDa Vibrio cholerae serotype … protein
Buffer: 50 mM Na2HPO4, 300 mM NaCl, 3% glycerol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Mar 6
Vibrio cholerae's ToxRS bile sensing system. Elife 12 (2023)
Gubensäk N, Sagmeister T, Buhlheller C, Geronimo BD, Wagner GE, Petrowitsch L, Gräwert MA, Rotzinger M, Berger TMI, Schäfer J, Usón I, Reidl J, Sánchez-Murcia PA, Zangger K, Pavkov-Keller T
RgGuinier 2.1 nm
Dmax 6.6 nm
VolumePorod 51 nm3

SASDR55 – A complex between the periplasmic domains of cholera toxin transcriptional activator ToxR and transmembrane regulatory protein ToxS bound to bile salt

Cholera toxin transcriptional activatorTransmembrane regulatory protein ToxSbile acid: sodium cholate hydrate experimental SAS data
CUSTOM IN-HOUSE model
Sample: Cholera toxin transcriptional activator monomer, 12 kDa Vibrio cholerae serotype … protein
Transmembrane regulatory protein ToxS dimer, 37 kDa Vibrio cholerae serotype … protein
bile acid: sodium cholate hydrate monomer, 0 kDa
Buffer: 50 mM Na2HPO4, 300 mM NaCl, 3% glycerol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Jul 7
Vibrio cholerae's ToxRS bile sensing system. Elife 12 (2023)
Gubensäk N, Sagmeister T, Buhlheller C, Geronimo BD, Wagner GE, Petrowitsch L, Gräwert MA, Rotzinger M, Berger TMI, Schäfer J, Usón I, Reidl J, Sánchez-Murcia PA, Zangger K, Pavkov-Keller T
RgGuinier 2.4 nm
Dmax 6.6 nm
VolumePorod 60 nm3

SASDSG6 – Human Ubiquilin-2, ∆STI1-2 domain deletion (UBQLN2∆STI1-2)

Ubiquilin-2 (∆379-462) experimental SAS data
Ubiquilin-2 (∆379-462) Kratky plot
Sample: Ubiquilin-2 (∆379-462) monomer, 56 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jun 8
Short disordered N-termini & proline-rich domain are major regulators of UBQLN1/2/4 phase separation.
Sarasi Galagedera
RgGuinier 4.4 nm
Dmax 22.4 nm
VolumePorod 121 nm3

SASDSH6 – Human Ubiquilin-2, PXX domain deletion (UBQLN2∆PXX)

Ubiquilin-2 (∆487-538) experimental SAS data
Ubiquilin-2 (∆487-538) Kratky plot
Sample: Ubiquilin-2 (∆487-538) dimer, 122 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jun 8
Short disordered N-termini & proline-rich domain are major regulators of UBQLN1/2/4 phase separation.
Sarasi Galagedera
RgGuinier 4.8 nm
Dmax 19.5 nm
VolumePorod 288 nm3

4706 hits found.