Small Angle Scattering Biological Data Bank SASBDB

SASDMR7 – HOLO-serotransferrin at pH 7.0 in the presence of iron

Sample:	Serotransferrin monomer, 77 kDa Homo sapiens protein
Buffer:	15 mM HEPES, 20 mM NaHCO3, 50 mM NaCl (APO Buffer), pH: 7
Experiment:	SAXS data collected at BM29, ESRF on 2021 Apr 7

X-ray Characterization of Conformational Changes of Human Apo- and Holo-Transferrin International Journal of Molecular Sciences 22(24):13392 (2021)
Campos-Escamilla C, Siliqi D, Gonzalez-Ramirez L, Lopez-Sanchez C, Gavira J, Moreno A

R_g^Guinier	3.2	nm
D_max	13.5	nm
Volume^Porod	103	nm³

SASDMS7 – HOLO-serotransferrin at pH 8.0 in the presence of iron

Sample:	Serotransferrin monomer, 77 kDa Homo sapiens protein
Buffer:	15 mM HEPES, 20 mM NaHCO3, 50 mM NaCl (Buffer APO-Tf-1), pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2021 Apr 7

R_g^Guinier	3.2	nm
D_max	13.1	nm
Volume^Porod	106	nm³

SASDKV7 – CCP2-SP domains of the complement C1r subcomponent

Complement C1r subcomponent experimental SAS data

Sample:	Complement C1r subcomponent monomer, 38 kDa Homo sapiens protein
Buffer:	10 mM HEPES, 140 mM NaCl, pH: 7.3
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Sep 24

A Structural Basis for Inhibition of the Complement Initiator Protease C1r by Lyme Disease Spirochetes. J Immunol 207(11):2856-2867 (2021)
Garrigues RJ, Powell-Pierce AD, Hammel M, Skare JT, Garcia BL

R_g^Guinier	2.4	nm
D_max	7.9	nm
Volume^Porod	63	nm³

SASDKW7 – Complement inhibitory domain of the Borrelia burgdorferi fibronectin-binding protein BBK32

Fibronectin-binding protein BBK32 experimental SAS data

Sample:	Fibronectin-binding protein BBK32 monomer, 17 kDa Borreliella burgdorferi B31 protein
Buffer:	10 mM HEPES, 140 mM NaCl, pH: 7.3
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Sep 24

R_g^Guinier	2.1	nm
D_max	7.6	nm
Volume^Porod	34	nm³

SASDKX7 – CCP2-SP domains of the complement C1r subcomponent bound to the complement inhibitory domain of the Borrelia burgdorferi fibronectin-binding protein BBK32

Fibronectin-binding protein BBK32Complement C1r subcomponent experimental SAS data

Sample:	Fibronectin-binding protein BBK32 monomer, 17 kDa Borrelia burgdorferi (strain … protein Complement C1r subcomponent monomer, 38 kDa Homo sapiens protein
Buffer:	10 mM HEPES, 140 mM NaCl, pH: 7.3
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Sep 24

R_g^Guinier	2.8	nm
D_max	8.6	nm
Volume^Porod	75	nm³

SASDMR9 – Guanidine-II Riboswitch from Escherichia coli 23mer

Guanidin-II riboswitch 23mer experimental SAS data

Guanidin-II riboswitch 23mer Kratky plot

Sample:	Guanidin-II riboswitch 23mer monomer, 7 kDa Escherichia coli RNA
Buffer:	25 mM potassium phosphate, 50 mM KCl, pH: 6.2
Experiment:	SAXS data collected at BM29, ESRF on 2021 Feb 13

Structure and Dynamics of the Guanidine-II Riboswitch from Escherichia coli by NMR Spectroscopy and Small-angle X-ray Scattering (SAXS). Chembiochem (2021)
Schamber T, Binas O, Schlundt A, Wacker A, Schwalbe H

R_g^Guinier	1.5	nm
D_max	4.8	nm
Volume^Porod	14	nm³

SASDMS9 – Guanidine-II Riboswitch from Escherichia coli 23mer with Mg2+

Sample:	Guanidin-II riboswitch 23mer monomer, 7 kDa Escherichia coli RNA
Buffer:	25 mM potassium phosphate, 50 mM KCl, 10 mM Mg2+, pH: 6.2
Experiment:	SAXS data collected at BM29, ESRF on 2021 Feb 13

R_g^Guinier	1.8	nm
D_max	5.8	nm
Volume^Porod	15	nm³

SASDMT9 – Guanidine-II Riboswitch from Escherichia coli 23mer with Mg2+ and Gdn ligand

Sample:	Guanidin-II riboswitch 23mer monomer, 7 kDa Escherichia coli RNA
Buffer:	25 mM potassium phosphate, 50 mM KCl, 10 mM Mg2+, 5 mM GdnCl, pH: 6.2
Experiment:	SAXS data collected at BM29, ESRF on 2021 Feb 13

R_g^Guinier	2.2	nm
D_max	8.8	nm
Volume^Porod	19	nm³

SASDMU9 – Guanidine-II Riboswitch from Escherichia coli 49mer

Guanidin-II riboswitch 49mer experimental SAS data

Guanidin-II riboswitch 49mer Kratky plot

Sample:	Guanidin-II riboswitch 49mer dimer, 32 kDa Escherichia coli RNA
Buffer:	25 mM potassium phosphate, 50 mM KCl, pH: 6.2
Experiment:	SAXS data collected at BM29, ESRF on 2021 Feb 13

R_g^Guinier	4.1	nm
D_max	16.9	nm
Volume^Porod	56	nm³

SASDMV9 – Guanidine-II Riboswitch from Escherichia coli 49mer with Mg2+

Sample:	Guanidin-II riboswitch 49mer dimer, 32 kDa Escherichia coli RNA
Buffer:	25 mM potassium phosphate, 50 mM KCl, 10 mM Mg2+, pH: 6.2
Experiment:	SAXS data collected at BM29, ESRF on 2021 Feb 13

R_g^Guinier	4.2	nm
D_max	18.1	nm
Volume^Porod	57	nm³

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